Mouse Anti-DDX20 Recombinant Antibody (D0538) (CBMAB-D0538-YC)

Name: Mouse Anti-DDX20 Recombinant Antibody (D0538)
SKU: CBMAB-D0538-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DDX20 Recombinant Antibody (D0538) (CBMAB-D0538-YC-1) in IP

In vitro transcribed U2 snRNA (WT and mutants) was radioactively labeled and incubated in cytoplasmic extracts prepared from control cells or anti-Gemin3 siRNA-treated cells. Sm protein association with snRNA was assayed by immunoprecipitation of Sm proteins followed by autoradiography of co-precipitated snRNAs. ...View More

Pánek, J., Roithová, A., Radivojević, N., Sýkora, M., Prusty, A. B., Huston, N., ... & Staněk, D. (2023). The SMN complex drives structural changes in human snRNAs to enable snRNP assembly. Nature communications, 14(1), 6580.

Mouse Anti-DDX20 Recombinant Antibody (D0538) (CBMAB-D0538-YC-2) in WB

Total cell lysates of P5 control and SMNdelta7 spinal cords and quadriceps, P2 control and mutSMA gastrocnemius, and 48 h cultured control, SMAII, and SMAI patient fibroblasts, were submitted to western blot analysis using anti-Gemin3 and anti-SMN antibodies. Membranes were reprobed using anti-α-tubulin antibody. Graph values represent the expression of Gemin3 versus α-tubulin and correspond to the quantification of at least four independent experiments ± SEM. ...View More

Miralles, M. P., Sansa, A., Beltran, M., Soler, R. M., & Garcera, A. (2022). Survival motor neuron protein and neurite degeneration are regulated by Gemin3 in spinal muscular atrophy motoneurons. Frontiers in Cellular Neuroscience, 16, 1054270.

Mouse Anti-DDX20 Recombinant Antibody (D0538) (CBMAB-D0538-YC-3) in IF

RIPosomes co-localization with cellular compartments and signalling platforms.
Indirect immunofluorescence micrographs of HeLa EGFP-RIPK2 cells infected with S. flexneri M90T or BS176 for 2 h. TIFA-Flag and Flag-TRAF6 were transfected 16 h before doxycycline induction. EGFP-RIPK2 (green), immunostaining with anti-Flag, anti-AIF, anti-SMN, anti-Gemin3, anti-LC3β, or anti-EEA1 antibodies (shown in red) together with DNA staining (blue) are shown. Scale bar = 10 μm. ...View More

Ellwanger, K., Briese, S., Arnold, C., Kienes, I., Heim, V., Nachbur, U., & Kufer, T. A. (2019). XIAP controls RIPK2 signaling by preventing its deposition in speck-like structures. Life Science Alliance, 2(4).

Mouse Anti-DDX20 Recombinant Antibody (D0538) (CBMAB-D0538-YC-4) in WB and IP

Co-immunoprecipitation of Gemin3 with αB-crystallin.
Extracts of HeLa cells, transfected with pIRES as a control or pIRES constructs coding for wild-type (WT), non-phosphorylatable αB-crystallin (αB-STA) or pseudophosphorylated αB-crystallin (αB-STD) were subjected to immunoprecipitation with a monoclonal antibody against αB-crystallin. The immunoprecipitates were analyzed by immunoblotting using a mouse monoclonal antibody against Gemin3 and rabbit polyclonal antibodies to αB-crystallin. Note that Gemin3 shows two bands, of which the lower band is likely a degradation product. ...View More

den Engelsman, J., van de Schootbrugge, C., Yong, J., Pruijn, G. J., & Boelens, W. C. (2013). Pseudophosphorylated αB-crystallin is a nuclear chaperone imported into the nucleus with help of the SMN complex. PloS one, 8(9), e73489.

Mouse Anti-DDX20 Recombinant Antibody (D0538) (CBMAB-D0538-YC-5) in WB and IP

Huh7 cells were transiently transfected with a control vector or a myc-tagged RACK1-expressing plasmid with or without a flag-tagged Dicer-expressing plasmid. Myc-tagged RACK1 was immunoprecipitated using anti-myc agarose. Normal mouse IgG was used as a control for immunoprecipitation. Co-precipitated proteins were blotted using antibodies against the indicated proteins. Five percent of the total cell lysates was loaded as "input." Representative results from two independent experiments are shown. Similar results were obtained using 293T cells. ...View More

Otsuka, M., Takata, A., Yoshikawa, T., Kojima, K., Kishikawa, T., Shibata, C., ... & Koike, K. (2011). Receptor for activated protein kinase C: requirement for efficient microRNA function and reduced expression in hepatocellular carcinoma. PLoS One, 6(9), e24359.

Mouse Anti-DDX20 Recombinant Antibody (D0538) (CBMAB-D0538-YC-6) in WB

WB analysis of WRAP53, SMN, Gemin3, and importinβ levels in fibroblasts from the same individuals. ...View More

Mahmoudi, S., Henriksson, S., Weibrecht, I., Smith, S., Söderberg, O., Strömblad, S., ... & Farnebo, M. (2010). WRAP53 is essential for Cajal body formation and for targeting the survival of motor neuron complex to Cajal bodies. PLoS biology, 8(11), e1000521.

Mouse Anti-DDX20 Recombinant Antibody (D0538) (CBMAB-D0538-YC-7) in IF

A and B, immunofluorescence analysis of HeLa cells infected with Shigella for 4 h and stained with antibodies against DDX20 and SMN (A) or polyclonal DDX20 and Sm (B). ...View More

Tsalikis, J., Tattoli, I., Ling, A., Sorbara, M. T., Croitoru, D. O., Philpott, D. J., & Girardin, S. E. (2015). Intracellular bacterial pathogens trigger the formation of U small nuclear RNA bodies (U bodies) through metabolic stress induction. Journal of Biological Chemistry, 290(34), 20904-20918.

Mouse Anti-DDX20 Recombinant Antibody (D0538) (CBMAB-D0538-YC-8) in WB

Reduction of SMN by RNA interference reveals SMN-independent subunits.A, SMN, Gemin2, and Gemin5 were immunoprecipitated from extracts from both a cell line stably expressing shRNA targeting SMN (lanes 4-6) and a control cell line expressing nontargeting shRNA (lanes 1-3). Immunoprecipitated proteins were observed by Western analysis. B, Gemin6 was immunoprecipitated as from cells expressing shRNA as described in A. Control immunoprecipitations were performed using mouse nonimmune antibody. ...View More

Battle, D. J., Kasim, M., Wang, J., & Dreyfuss, G. (2007). SMN-independent subunits of the SMN complex: identification of a small nuclear ribonucleoprotein assembly intermediate. Journal of Biological Chemistry, 282(38), 27953-27959.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D0538

Application

WB, IP, IF, IHC-P

Immunogen

Amino acids 368-548 of human Gemin3.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

DEAD-Box Helicase 20

Introduction

DDX20 is a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. DDX20 interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Entrez Gene ID

Human	11218
Rat	84473

UniProt ID

Human	Q9UHI6
Rat	F1MAM8

Alternative Names

DEAD-Box Helicase 20; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 20, 103kD; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20; DEAD Box Protein DP 103; Component Of Gems 3; DEAD Box Protein 20; Gemin-3;

Function

The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).

Biological Process

Import into nucleus Source: Reactome
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of apoptotic process Source: MGI
RNA processing Source: ProtInc
Spliceosomal snRNP assembly Source: UniProtKB
Spliceosomal tri-snRNP complex assembly Source: ProtInc

Cellular Location

Gem; Cytoplasm. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).

Bizen, N., Bepari, A. K., Zhou, L., Abe, M., Sakimura, K., Ono, K., & Takebayashi, H. (2022). Ddx20, an Olig2 binding factor, governs the survival of neural and oligodendrocyte progenitor cells via proper Mdm2 splicing and p53 suppression. Cell Death & Differentiation, 1-14.

Simankova, A., Bizen, N., Saitoh, S., Shibata, S., Ohno, N., Abe, M., ... & Takebayashi, H. (2021). Ddx20, DEAD box helicase 20, is essential for the differentiation of oligodendrocyte and maintenance of myelin gene expression. Glia, 69(11), 2559-2574.

Bourova-Flin, E., Derakhshan, S., Goudarzi, A., Wang, T., Vitte, A. L., Chuffart, F., ... & Aminishakib, P. (2021). The combined detection of Amphiregulin, Cyclin A1 and DDX20/Gemin3 expression predicts aggressive forms of oral squamous cell carcinoma. British Journal of Cancer, 125(8), 1122-1134.

Huang, Y., Wang, C., Li, K., Ye, Y., Shen, A., Guo, L., ... & Lin, Y. (2020). Death‐associated protein kinase 1 suppresses hepatocellular carcinoma cell migration and invasion by upregulation of DEAD‐box helicase 20. Cancer science, 111(8), 2803-2813.

Wang, Q., Ye, Y., Lin, R., Weng, S., Cai, F., Zou, M., ... & Lin, Y. (2020). Analysis of the expression, function, prognosis and co-expression genes of DDX20 in gastric cancer. Computational and structural biotechnology journal, 18, 2453-2462.

Curmi, F., & Cauchi, R. J. (2018). The multiple lives of DEAD-box RNA helicase DP103/DDX20/Gemin3. Biochemical Society Transactions, 46(2), 329-341.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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