Mouse Anti-DNMT3A Recombinant Antibody (D1421) (V2LY-0425-LY726)

Name: Mouse Anti-DNMT3A Recombinant Antibody (D1421)
SKU: V2LY-0425-LY726
Rating: 5 (1 reviews)

See all DNMT3A antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D1421

Application

Immunogen

E. coli-derived recombinant human DNMT3A (Ala353-Lys486).

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	0.1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

Trehalose & PBS

Preservative

None

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DNA Methyltransferase 3 Alpha

Entrez Gene ID

Human	1788
Mouse	13435

UniProt ID

Human	Q9Y6K1
Mouse	O88508

Function

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed:12138111, PubMed:16357870, PubMed:30478443).

DNA methylation is coordinated with methylation of histones (PubMed:12138111, PubMed:16357870, PubMed:30478443).

It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed:12138111, PubMed:16357870, PubMed:30478443).

May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity).

Plays a role in paternal and maternal imprinting (By similarity).

Required for methylation of most imprinted loci in germ cells (By similarity).

Acts as a transcriptional corepressor for ZBTB18 (By similarity).

Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity).

Can actively repress transcription through the recruitment of HDAC activity (By similarity).

Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity).

Biological Process

Aging Source: Ensembl
Cellular response to amino acid stimulus Source: Ensembl
Cellular response to ethanol Source: Ensembl
Cellular response to hypoxia Source: Ensembl
DNA methylation Source: UniProtKB
DNA methylation-dependent heterochromatin assembly Source: Ensembl
DNA methylation involved in embryo development Source: Ensembl
DNA methylation involved in gamete generation Source: Ensembl
Hepatocyte apoptotic process Source: Ensembl
Mitotic cell cycle Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Neuron differentiation Source: Ensembl
Positive regulation of cell death Source: Ensembl
Regulation of gene expression by genetic imprinting Source: UniProtKB
Response to cocaine Source: Ensembl
Response to drug Source: Ensembl
Response to estradiol Source: Ensembl
Response to ionizing radiation Source: Ensembl
Response to lead ion Source: Ensembl
Response to toxic substance Source: Ensembl
Response to vitamin A Source: Ensembl
Spermatogenesis Source: Ensembl

Cellular Location

Nucleus; Cytoplasm; Chromosome. Accumulates in the major satellite repeats at pericentric heterochromatin.

Involvement in disease

Tatton-Brown-Rahman syndrome (TBRS):
An overgrowth syndrome characterized by a distinctive facial appearance, tall stature and intellectual disability. Facial gestalt is characterized by a round face, heavy horizontal eyebrows and narrow palpebral fissures. Less common features include atrial septal defects, seizures, umbilical hernia, and scoliosis.
Leukemia, acute myelogenous (AML):
A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes.
Heyn-Sproul-Jackson syndrome (HESJAS):
An autosomal dominant form of microcephalic dwarfism. Affected individuals have intrauterine growth retardation, postnatal growth restrictions, proportionate short stature, microcephaly, severe developmental delay and impaired intellectual development. More variable features include sparse hair, short broad metacarpals and phalanges, and mild recurrent infections.

PTM

Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
Auto-methylated at Cys-710: auto-methylation takes place in absence of DNA substrate and inactivates the DNA methyltransferase activity. Inactivation by auto-methylation may be used to inactivate unused DNA methyltransferases in the cell.

Loughland, I., Little, A., & Seebacher, F. (2021). DNA methyltransferase 3a mediates developmental thermal plasticity. BMC biology, 19(1), 1-11.

Small, L., Ingerslev, L. R., Manitta, E., Laker, R. C., Hansen, A. N., Deeney, B., ... & Barrès, R. (2021). Ablation of DNA-methyltransferase 3A in skeletal muscle does not affect energy metabolism or exercise capacity. PLoS genetics, 17(1), e1009325.

Wei, J., Cheng, J., Waddell, N. J., Wang, Z. J., Pang, X., Cao, Q., ... & Yan, Z. (2021). DNA methyltransferase 3A is involved in the sustained effects of chronic stress on synaptic functions and behaviors. Cerebral Cortex, 31(4), 1998-2012.

Wei, D., Yu, G., & Zhao, Y. (2019). MicroRNA-30a-3p inhibits the progression of lung cancer via the PI3K/AKT by targeting DNA methyltransferase 3a. OncoTargets and therapy, 12, 7015.

Zhang, Z. M., Lu, R., Wang, P., Yu, Y., Chen, D., Gao, L., ... & Song, J. (2018). Structural basis for DNMT3A-mediated de novo DNA methylation. Nature, 554(7692), 387-391.

Cui, H., Hu, Y., Guo, D., Zhang, A., Gu, Y., Zhang, S., ... & Fan, H. (2018). DNA methyltransferase 3A isoform b contributes to repressing E-cadherin through cooperation of DNA methylation and H3K27/H3K9 methylation in EMT-related metastasis of gastric cancer. Oncogene, 37(32), 4358-4371.

Wang, L., Yao, J., Sun, H., He, K., Tong, D., Song, T., & Huang, C. (2017). MicroRNA‑101 suppresses progression of lung cancer through the PTEN/AKT signaling pathway by targeting DNA methyltransferase 3A. Oncology letters, 13(1), 329-338.

Cole, C. B., Russler-Germain, D. A., Ketkar, S., Verdoni, A. M., Smith, A. M., Bangert, C. V., ... & Ley, T. J. (2017). Haploinsufficiency for DNA methyltransferase 3A predisposes hematopoietic cells to myeloid malignancies. The Journal of clinical investigation, 127(10), 3657-3674.

Shao, Z., Xu, P., Xu, W., Li, L., Liu, S., Zhang, R., ... & Luo, C. (2017). Discovery of novel DNA methyltransferase 3A inhibitors via structure-based virtual screening and biological assays. Bioorganic & Medicinal Chemistry Letters, 27(2), 342-346.

Jia, Y., Li, T., Huang, X., Xu, X., Zhou, X., Jia, L., ... & Duan, T. (2017). Dysregulated DNA methyltransferase 3A upregulates IGFBP5 to suppress trophoblast cell migration and invasion in preeclampsia. Hypertension, 69(2), 356-366.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-DNMT3A Recombinant Antibody (D1421)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-DNMT3A Recombinant Antibody (15B82) (CAT#: CBMAB-D1415-YC)

Mouse Anti-DNMT3A Recombinant Antibody (D1416) (CAT#: CBMAB-D1416-YC)

Rabbit Anti-DNMT3A Recombinant Antibody (CP0484) (CAT#: CBMAB-CP0484-LY)

Mouse Anti-DNMT3A Recombinant Antibody (D1418) (CAT#: V2LY-0425-LY724)

Mouse Anti-DNMT3A Recombinant Antibody (CBT2382) (CAT#: V2LY-0625-LY489)

Mouse Anti-DNMT3A Recombinant Antibody (CBYCD-365) (CAT#: V2LY-0425-LY725)

Mouse Anti-DNMT3A Antibody (1E2) (CAT#: CBMAB-0298-YC)

Mouse Anti-DNMT3A Recombinant Antibody (D1422) (CAT#: V2LY-0425-LY727)

Mouse Anti-DNMT3A Monoclonal Antibody (1E2) (CAT#: V2LY-0425-LY720)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry