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Mouse Anti-EIF5B Recombinant Antibody (3F9) (CBMAB-A2555-LY)

The product is antibody recognizes EIF5B. The antibody 3F9 immunoassay techniques such as: sELISA, ELISA.
See all EIF5B antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3F9
Antibody Isotype
IgG2a, κ
Application
sELISA, ELISA

Basic Information

Immunogen
EIF5B (NP_056988, 1121 a.a. ~ 1218 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Eukaryotic Translation Initiation Factor 5B
Introduction
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq]
Entrez Gene ID
9669
UniProt ID
O60841
Alternative Names
DKFZp434I036; FLJ10524; IF2; KIAA0741
Research Area
Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.
Biological Process
Regulation of translational initiation Source: UniProtKB
Cellular Location
Cytoplasm
PTM
(Microbial infection) Cleaved and inactivated by the protease 3C of poliovirus, Coxsackievirus B3 and Human rhinovirus 14, allowing the virus to shutoff the host cell translation.

Lapointe, C. P., Grosely, R., Sokabe, M., Alvarado, C., Wang, J., Montabana, E., ... & Puglisi, J. D. (2022). eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining. Nature, 607(7917), 185-190.

Li, Q., Xiao, M., Shi, Y., Hu, J., Bi, T., Wang, C., ... & Li, X. (2021). eIF5B regulates the expression of PD-L1 in prostate cancer cells by interacting with Wig1. BMC cancer, 21(1), 1-11.

Huang, B. Y., & Fernández, I. S. (2020). Long-range interdomain communications in eIF5B regulate GTP hydrolysis and translation initiation. Proceedings of the National Academy of Sciences, 117(3), 1429-1437.

Suresh, S., Chen, B., Zhu, J., Golden, R. J., Lu, C., Evers, B. M., ... & O’Donnell, K. A. (2020). eIF5B drives integrated stress response-dependent translation of PD-L1 in lung cancer. Nature cancer, 1(5), 533-545.

Wang, J., Wang, J., Shin, B. S., Kim, J. R., Dever, T. E., Puglisi, J. D., & Fernández, I. S. (2020). Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex. Nature communications, 11(1), 1-8.

Wang, J., Johnson, A. G., Lapointe, C. P., Choi, J., Prabhakar, A., Chen, D. H., ... & Puglisi, J. D. (2019). eIF5B gates the transition from translation initiation to elongation. Nature, 573(7775), 605-608.

Ross, J. A., Dungen, K. V., Bressler, K. R., Fredriksen, M., Khandige Sharma, D., Balasingam, N., & Thakor, N. (2019). Eukaryotic initiation factor 5B (eIF5B) provides a critical cell survival switch to glioblastoma cells via regulation of apoptosis. Cell death & disease, 10(2), 1-15.

Kim, E., Kim, J. H., Seo, K., Hong, K. Y., An, S. W. A., Kwon, J., ... & Jang, S. K. (2018). eIF2A, an initiator tRNA carrier refractory to eIF2α kinases, functions synergistically with eIF5B. Cellular and Molecular Life Sciences, 75(23), 4287-4300.

Lin, K. Y., Nag, N., Pestova, T. V., & Marintchev, A. (2018). Human eIF5 and eIF1A compete for binding to eIF5B. Biochemistry, 57(40), 5910-5920.

Ross, J. A., Bressler, K. R., & Thakor, N. (2018). Eukaryotic initiation factor 5B (eIF5B) cooperates with eIF1A and eIF5 to facilitate uORF2-mediated repression of ATF4 translation. International journal of molecular sciences, 19(12), 4032.

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For research use only. Not intended for any clinical use.

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