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Mouse Anti-EMC8 Recombinant Antibody (CBFYC-2600) (V2LY-0825-LY144)

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Basic Information

Host Animal
Mouse
Clone
CBFYC-2600
Application
WB, IF
Immunogen
Full length human recombinant protein of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, glycerol, BSA
Preservative
Sodium azide
Concentration
1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
ER Membrane Protein Complex Subunit 8
Entrez Gene ID
Human10328
Mouse18117
Rat361425
UniProt ID
HumanO43402
MouseO70378
RatQ5FVL2
Research Area
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656).

Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231).

Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151).

It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231).

By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835).

By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).
Biological Process
Protein insertion into ER membrane by stop-transfer membrane-anchor sequence Source: UniProtKB
Tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane

Iyer, A., Niemann, M., Serricchio, M., Dewar, C. E., Oeljeklaus, S., Farine, L., ... & Bütikofer, P. (2022). The endoplasmic reticulum membrane protein complex localizes to the mitochondrial-endoplasmic reticulum interface and its subunits modulate phospholipid biosynthesis in Trypanosoma brucei. PLoS pathogens, 18(5), e1009717.

Guna, A., Hazu, M., Tomaleri, G. P., & Voorhees, R. M. (2022). A TAle of Two Pathways: Tail-Anchored Protein Insertion at the Endoplasmic Reticulum. Cold Spring Harbor Perspectives in Biology, a041252.

Bai, L., & Li, H. (2022). Cryo‐EM structures of the endoplasmic reticulum membrane complex. The FEBS Journal, 289(1), 102-112.

Alvira, S., Corey, R. A., Collinson, I., & Römisch, K. (2021). Membrane protein biogenesis by the EMC. The EMBO Journal, 40(2), e107407.

Yang, M., Li, S., Liu, W., Li, X., He, Y., Yang, Y., ... & Zhu, X. (2021). The ER membrane protein complex subunit Emc3 controls angiogenesis via the FZD4/WNT signaling axis. Science China Life Sciences, 64(11), 1868-1883.

Pleiner, T., Tomaleri, G. P., Januszyk, K., Inglis, A. J., Hazu, M., & Voorhees, R. M. (2020). Structural basis for membrane insertion by the human ER membrane protein complex. Science, 369(6502), 433-436.

Pleiner, T., Januszyk, K., Tomaleri, G. P., Oania, R. S., Hazu, M., Sweredoski, M. J., ... & Voorhees, R. M. (2020). Regulated assembly of the ER membrane protein complex. bioRxiv.

Volkmar, N., Thezenas, M. L., Louie, S. M., Juszkiewicz, S., Nomura, D. K., Hegde, R. S., ... & Christianson, J. C. (2019). The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis. Journal of Cell Science, 132(2), jcs223453.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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