Mouse Anti-ENPP3 Recombinant Antibody (CBT2181) (V2LY-0625-LY1016)

Name: Mouse Anti-ENPP3 Recombinant Antibody (CBT2181)
SKU: V2LY-0625-LY1016
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-ENPP3 Recombinant Antibody (CBT2181) (V2LY-0625-LY1016) in ELISA

Mouse Anti-ENPP3 Recombinant Antibody (CBT2181) (V2LY-0625-LY1016) in WB

Mouse Anti-ENPP3 Recombinant Antibody (CBT2181) (V2LY-0625-LY1016) in FC

Mouse Anti-ENPP3 Recombinant Antibody (CBT2181) (V2LY-0625-LY1016) in IHC

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBT2181

Antibody Isotype

IgG1

Application

IHC, FC

Basic Information

Immunogen

Purified recombinant fragment of human CD203C (AA: extra 45-163) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

ectonucleotide pyrophosphatase/phosphodiesterase 3

Entrez Gene ID

5169

UniProt ID

O14638

Research Area

Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP (PubMed:29717535).

Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (By similarity).

Has also alkaline phosphodiesterase activity (PubMed:11342463).

Biological Process

ATP metabolic process Source: UniProtKB
Basophil activation involved in immune response Source: UniProtKB
Negative regulation of inflammatory response Source: UniProtKB
Negative regulation of mast cell activation involved in immune response Source: UniProtKB
Negative regulation of mast cell proliferation Source: UniProtKB
Nucleoside triphosphate catabolic process Source: BHF-UCL
Phosphate-containing compound metabolic process Source: BHF-UCL
Pyrimidine nucleotide metabolic process Source: UniProtKB

Cellular Location

Cell membrane; Apical cell membrane; Secreted. Detected at the cell surface of basophils (PubMed:11342463). Detected at the apical plasma membrane of bile duct cells (PubMed:15072822). Located to the apical surface in intestinal and kidney epithelial cells. Secreted in serum, and in lumen of epithelial cells.

Topology

Cytoplasmic: 1-11
Helical: 12-30
Extracellular: 31-875

PTM

N-glycosylated. N-glycosylation is necessary for normal transport to the cell membrane, but is not the apical targeting signal.

More Infomation

References

Ullah, S., Pelletier, J., Sévigny, J., & Iqbal, J. (2022). Synthesis and Biological Evaluation of Arylamide Sulphonate Derivatives as Ectonucleotide Pyrophosphatase/Phosphodiesterase-1 and-3 Inhibitors. ACS omega, 7(30), 26905-26918.

Nisthal, A., Lee, S. H., Kim, Y. K., Bonzon, C., Rashid, R., Avery, K. N., ... & Desjarlais, J. R. (2020). XmAb30819, an XmAb® 2+ 1 ENPP3 x CD3 bispecific antibody for RCC, demonstrates safety and efficacy in in vivo preclinical studies. Cancer Research, 80(16_Supplement), 2286-2286.

Trapero, C., Jover, L., Fernández‐Montolí, M. E., García‐Tejedor, A., Vidal, A., Gomez de Aranda, I., ... & Martín‐Satué, M. (2018). Analysis of the ectoenzymes ADA, ALP, ENPP1, and ENPP3, in the contents of ovarian endometriomas as candidate biomarkers of endometriosis. American Journal of Reproductive Immunology, 79(2), e12794.

Chen, Q., Xin, A., Qu, R., Zhang, W., Li, L., Chen, J., ... & Sun, X. (2018). Expression of ENPP3 in human cyclic endometrium: a novel molecule involved in embryo implantation. Reproduction, Fertility and Development, 30(10), 1277-1285.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

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Mouse Anti-ENPP3 (AA 602-700) Recombinant Antibody (CBFYE-0892)

Mouse Anti-ENPP3 Recombinant Antibody (CBXC-3136)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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