Mouse Anti-EPHA1 Recombinant Antibody (836) (CBMAB-E1451-FY)

Name: Mouse Anti-EPHA1 Recombinant Antibody (836)
SKU: CBMAB-E1451-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

836

Application

Immunogen

Recombinant human EphA1 (Arg24-Glu547).

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, trehalose

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Epitope

AA 24-547

More Infomation

Target

Full Name

EPH Receptor A1

Introduction

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis.

Entrez Gene ID

2041

UniProt ID

P21709

Alternative Names

EPH Receptor A1; Erythropoietin-Producing Hepatoma Receptor; Tyrosine-Protein Kinase Receptor EPH; EC 2.7.10.1; HEpha1; EPHT1; EPHT; EPH; Erythropoietin-Producing Hepatoma Amplified Sequence; Ephrin Type-A Receptor 1

Research Area

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.

Biological Process

Activation of GTPase activity Source: UniProtKB
Angiogenesis Source: UniProtKB-KW
Axon guidance Source: GO_Central
Cell surface receptor signaling pathway Source: UniProtKB
Negative regulation of cell migration Source: UniProtKB
Negative regulation of protein kinase activity Source: UniProtKB
Peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of cell-matrix adhesion Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Positive regulation of stress fiber assembly Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Regulation of GTPase activity Source: UniProtKB
Substrate adhesion-dependent cell spreading Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Cellular Location

Cell membrane

Topology

Extracellular: 26-547
Helical: 548-568
Cytoplasmic: 569-976

PTM

Phosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.
Ubiquitinated.

Koh, H. M., Jang, B. G., Lee, D. H., Hyun, C. L., & Kim, D. C. (2022). The association between ephrin receptor-A1 expression and survival in patients with cancer: a meta-analysis. Translational Cancer Research, 11(6), 1587.

Wu, Y., Du, Z., Mou, J., Qiu, X., Chen, J., Cai, S., ... & Yuan, C. (2022). The Functions of EphA1 receptor tyrosine kinase in Several Tumors. Current Medicinal Chemistry.

Kim, Y., Lasso, G., Patel, H., Vardarajan, B., Santa-Maria, I., & Lefort, R. (2021). Alzheimer’s disease-associated P460L mutation in ephrin receptor type A1 (EphA1) leads to dysregulated Rho-GTPase signaling. bioRxiv.

Gajdzis, M., Theocharis, S., Gajdzis, P., Cassoux, N., Gardrat, S., Donizy, P., ... & Kaczmarek, R. (2020). Ephrin receptors (EPH): EphA1, EphA5, and EphA7 expression in uveal melanoma—associations with clinical parameters and patient survival. Life, 10(10), 225.

Ieguchi, K., & Maru, Y. (2019). Roles of EphA1/A2 and ephrin‐A1 in cancer. Cancer science, 110(3), 841-848.

Jin, Y., Zou, Y., Wan, L., Lu, M., Liu, Y., Huang, G., ... & Xi, Q. (2018). Decreased Eph receptor‑A1 expression is related to grade in ovarian serous carcinoma. Molecular Medicine Reports, 17(4), 5409-5415.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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