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Mouse Anti-EPHA5 (AA 58-573) Recombinant Antibody (CBFYE-0120) (CBMAB-E0242-FY)

This product is mouse antibody that recognizes EPHA5. The antibody CBFYE-0120 can be used for immunoassay techniques such as: WB, FC, ICC, CyTOF.
See all EPHA5 antibodies
Published Data

Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
CBFYE-0120
Antibody Isotype
IgG1
Application
WB, FC, ICC, CyTOF

Basic Information

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat EphA5, Ser58-Gln573
Specificity
Human, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, trehalose
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 58-573

Target

Full Name
EPH Receptor A5
Introduction
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described.
Entrez Gene ID
Human2044
Rat79208
UniProt ID
HumanP54756
RatP54757
Alternative Names
EPH Receptor A5; Brain-Specific Kinase; EPH Homology Kinase 1; EPH-Like Kinase 7; EC 2.7.10.1; TYRO4; EHK-1; EHK1; HEK7
Research Area
Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity).
Biological Process
Axon guidance Source: UniProtKB
cAMP-mediated signaling Source: UniProtKB
Ephrin receptor signaling pathway Source: UniProtKB
Hippocampus development Source: UniProtKB
Neuron development Source: UniProtKB
Positive regulation of CREB transcription factor activity Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Regulation of actin cytoskeleton organization Source: UniProtKB
Regulation of GTPase activity Source: UniProtKB
Regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Cellular Location
Cell membrane; Axon; Dendrite
Topology
Extracellular: 25-573
Helical: 574-594
Cytoplasmic: 595-1037
PTM
Phosphorylated. Phosphorylation is stimulated by the ligand EFNA5. Dephosphorylation upon stimulation by glucose, inhibits EPHA5 forward signaling and results in insulin secretion (By similarity).

Li, Z., Zhou, Q., Wang, Q., Wang, H., & Yue, W. (2022). EPHA5 mutation was associated with adverse outcome of atezolizumab treatment in late-stage non-small cell lung cancers. BMC pulmonary medicine, 22(1), 1-8.

Li, J., & Zhu, Y. (2021). EPHA5 enhances the stemness of non-small cell lung cancer cells through activating the Wnt signaling pathway. Journal of BU ON.: Official Journal of the Balkan Union of Oncology, 26(5), 1871-1878.

Huang, W., Lin, A., Luo, P., Liu, Y., Xu, W., Zhu, W., ... & Zhang, J. (2021). EPHA5 mutation predicts the durable clinical benefit of immune checkpoint inhibitors in patients with lung adenocarcinoma. Cancer gene therapy, 28(7), 864-874.

Liu, L., Feng, X., Li, H., Cheng Li, S., Qian, Q., & Wang, Y. (2021). Deep learning model reveals potential risk genes for ADHD, especially Ephrin receptor gene EPHA5. Briefings in Bioinformatics, 22(6), bbab207.

Zhang, R., Liu, J., Zhang, W., Hua, L., Qian, L. T., & Zhou, S. B. (2020). EphA5 knockdown enhances the invasion and migration ability of esophageal squamous cell carcinoma via epithelial-mesenchymal transition through activating Wnt/β-catenin pathway. Cancer Cell International, 20(1), 1-12.

Gajdzis, M., Theocharis, S., Gajdzis, P., Cassoux, N., Gardrat, S., Donizy, P., ... & Kaczmarek, R. (2020). Ephrin receptors (EPH): EphA1, EphA5, and EphA7 expression in uveal melanoma—associations with clinical parameters and patient survival. Life, 10(10), 225.

Suo, G., Shen, F., Sun, B., Song, H., Xu, M., & Wu, Y. (2018). Abnormal expression of ephrin-A5 affects brain development of congenital hypothyroidism rats. Neuroreport, 29(11), 877-882.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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