Rabbit Anti-ESRP1 Recombinant Antibody (CBFYE-1297) (CBMAB-E1893-FY)

Name: Rabbit Anti-ESRP1 Recombinant Antibody (CBFYE-1297)
SKU: CBMAB-E1893-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBFYE-1297

Application

IHC-P, WB

Immunogen

Recombinant fragment of human ESRP1.

Host Species

Rabbit

Specificity

Human, Mouse, Zebrafish

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Epithelial Splicing Regulatory Protein 1

Introduction

ESPR1 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009).

Entrez Gene ID

207920

UniProt ID

Q3US41

Alternative Names

Rbm35a; BC031468; A630065D16; 2210008M09Rik

Research Area

mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2 (PubMed:19285943).

Regulates splicing and expression of genes involved in inner ear development, auditory hair cell differentiation, and cell fate specification in the cochlear epithelium (By similarity).

Biological Process

mRNA processing Source: UniProtKB-KW
Regulation of inner ear auditory receptor cell fate specification Source: UniProtKB
Regulation of RNA splicing Source: UniProtKB
RNA splicing Source: UniProtKB-KW

Cellular Location

Nucleus

Involvement in disease

Deafness, autosomal recessive, 109 (DFNB109):
A form of non-syndromic, sensorineural deafness characterized by bilateral, congenital, severe to profound hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNB109 affected individuals additionally exhibit vestibular dysplasia, although they do not manifest problems with balance or movement.

Yu, L., Zhang, H., Guan, X., Qin, D., Zhou, J., & Wu, X. (2021). Loss of ESRP1 blocks mouse oocyte development and leads to female infertility. Development, 148(2), dev196931.

Zheng, M., Niu, Y., Bu, J., Liang, S., Zhang, Z., Liu, J., ... & Wang, Q. (2021). ESRP1 regulates alternative splicing of CARM1 to sensitize small cell lung cancer cells to chemotherapy by inhibiting TGF-β/Smad signaling. Aging (Albany NY), 13(3), 3554.

Yu, S., Wang, M., Zhang, H., Guo, X., & Qin, R. (2021). Circ_0092367 Inhibits EMT and Gemcitabine Resistance in Pancreatic Cancer via Regulating the miR-1206/ESRP1 Axis. Genes, 12(11), 1701.

Ashok, C., Ahuja, N., Natua, S., Mishra, J., Samaiya, A., & Shukla, S. (2021). E2F1 and epigenetic modifiers orchestrate breast cancer progression by regulating oxygen-dependent ESRP1 expression. Oncogenesis, 10(8), 1-10.

Deng, G., Zhou, X., Chen, L., Yao, Y., Li, J., Zhang, Y., ... & Tang, J. (2020). High expression of ESRP1 regulated by circ-0005585 promotes cell colonization in ovarian cancer. Cancer cell international, 20(1), 1-15.

Ahuja, N., Ashok, C., Natua, S., Pant, D., Cherian, A., Pandkar, M. R., ... & Shukla, S. (2020). Hypoxia-induced TGF-β–RBFOX2–ESRP1 axis regulates human MENA alternative splicing and promotes EMT in breast cancer. NAR cancer, 2(3), zcaa021.

Ala, U., Manco, M., Mandili, G., Tolosano, E., Novelli, F., Provero, P., ... & Fagoonee, S. (2020). Proteomics-based evidence for a pro-oncogenic role of ESRP1 in human colorectal cancer cells. International journal of molecular sciences, 21(2), 575.

Lee, S., Sears, M. J., Zhang, Z., Li, H., Salhab, I., Krebs, P., ... & Carstens, R. P. (2020). Cleft lip and cleft palate in Esrp1 knockout mice is associated with alterations in epithelial-mesenchymal crosstalk. Development, 147(21), dev187369.

Gökmen‐Polar, Y., Neelamraju, Y., Goswami, C. P., Gu, Y., Gu, X., Nallamothu, G., ... & Badve, S. S. (2019). Splicing factor ESRP 1 controls ER‐positive breast cancer by altering metabolic pathways. EMBO reports, 20(2), e46078.

Gokmen-Polar, Y., Gu, Y., Gu, X., & Badve, S. S. (2019). Abstract P5-04-13: Splicing factor ESRP1 controls ER-positive breast cancer progression by altering metabolic pathway genes. Cancer Research, 79(4_Supplement), P5-04.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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