Mouse Anti-EXTL3 Recombinant Antibody (CBFYE-1422) (V2LY-0825-LY984)

Name: Mouse Anti-EXTL3 Recombinant Antibody (CBFYE-1422)
SKU: V2LY-0825-LY984
Rating: 5 (1 reviews)

See all EXTL3 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-1422

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-300 mapping at the N-terminus of human EXTL3.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Exostosin Like Glycosyltransferase 3

Entrez Gene ID

2137

UniProt ID

O43909

Research Area

Glycosyltransferase which regulates the biosynthesis of heparan sulfate (HS). Important for both skeletal development and hematopoiesis, through the formation of HS proteoglycans (HSPGs) (PubMed:28132690, PubMed:28148688).

Required for the function of REG3A in regulating keratinocyte proliferation and differentiation (PubMed:22727489).

Biological Process

Heparan sulfate proteoglycan biosynthetic process Source: UniProtKB
Positive regulation of cell growth Source: Ensembl
Protein glycosylation Source: InterPro

Cellular Location

Golgi apparatus; Endoplasmic reticulum membrane

Involvement in disease

Immunoskeletal dysplasia with neurodevelopmental abnormalities (ISDNA):
An autosomal recessive disorder characterized by variable skeletal abnormalities and neurodevelopmental defects. Neurologic manifestations include intellectual disability and motor delay. Some patients manifest hypotonia and seizures. Skeletal features include disproportionate short stature, cervical malformations, epiphyseal and metaphyseal dysplasia, and rarely premature craniosynostosis with progressive microcephaly. Severe combined immunodeficiency with a complete absence of T cells is observed in some patients.

Topology

Cytoplasmic: 1-30
Helical: 31-51
Lumenal: 52-919

Chang, P., Chen, S., Chang, X., Zhu, J., Tang, Q., & Ma, L. (2022). EXTL3 could serve as a potential biomarker of prognosis and immunotherapy for prostate cancer and its potential mechanisms. European Journal of Medical Research, 27(1), 1-14.

Wilson, L. F. L., Dendooven, T., Hardwick, S. W., Echevarría-Poza, A., Tryfona, T., Krogh, K. B. R. M., ... & Dupree, P. (2022). The structure of EXTL3 helps to explain the different roles of bi-domain exostosins in heparan sulfate synthesis. Nature Communications, 13(1), 1-15.

Marques, C., Poças, J., Gomes, C., Faria-Ramos, I., Reis, C. A., Vivès, R. R., & Magalhães, A. (2022). Glycosyltransferases EXTL2 and EXTL3 cellular balance dictates Heparan Sulfate biosynthesis and shapes gastric cancer cell motility and invasion. Journal of Biological Chemistry, 102546.

Bajaj, S., Satoskar, P., Nair, A., Sheth, F., Sheth, J., & Sheth, H. (2022). An ultra-rare case of immunoskeletal dysplasia with neurodevelopmental abnormalities in an Indian patient with homozygous c. 953C> T variant in EXTL3 gene: a case report. BMC pediatrics, 22(1), 1-9.

Dupree, P., Dendooven, T., Hardwick, S., Tryfona, T., Chirgadze, D., & Luisi, B. (2022). The structure of EXTL3 helps to explain the different roles of bi-domain exostosins in heparan sulfate synthesis.

Akalın, A., Taskiran, E. Z., Şimşek‐Kiper, P. Ö., Utine, E., Alanay, Y., Özçelik, U., & Boduroğlu, K. (2021). Spondyloepimetaphyseal dysplasia EXTL3‐deficient type: Long‐term follow‐up and review of the literature. American Journal of Medical Genetics Part A, 185(10), 3104-3110.

Zhang, H., Corredor, A. L. G., Messina-Pacheco, J., Li, Q., Zogopoulos, G., Kaddour, N., ... & Gao, Z. H. (2021). REG3A/REG3B promotes acinar to ductal metaplasia through binding to EXTL3 and activating the RAS-RAF-MEK-ERK signaling pathway. Communications biology, 4(1), 1-13.

Yamada, S. (2020). Specific functions of Exostosin-like 3 (EXTL3) gene products. Cellular & molecular biology letters, 25(1), 1-12.

Aints, A., Mölder, S., & Salumets, A. (2019). EXTL3-interacting endometriosis-specific serum factors induce colony formation of endometrial stromal cells. Scientific reports, 9(1), 1-10.

Awad, W., Kjellström, S., Svensson Birkedal, G., Mani, K., & Logan, D. T. (2018). Structural and biophysical characterization of human EXTL3: domain organization, glycosylation, and solution structure. Biochemistry, 57(7), 1166-1177.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-EXTL3 Recombinant Antibody (CBFYE-1422)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry