Rabbit Anti-FADS1 Recombinant Antibody (
7A10) (V2LY-0725-LY967)

Name: Rabbit Anti-FADS1 Recombinant Antibody (7A10)
SKU: V2LY-0725-LY967
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-FADS1 Recombinant Antibody (7A10) (V2LY-0725-LY967) in WB

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate
All lanes: FADS1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52, 43 kDa
Observed band size: 55 kDa

Rabbit Anti-FADS1 Recombinant Antibody (7A10) (V2LY-0725-LY967) in IHC

IHC image of V2LY-0725-LY967 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-FADS1 Recombinant Antibody (7A10) (V2LY-0725-LY967) in IF

Immunofluorescence staining of HepG2 cell with V2LY-0725-LY967 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 565-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-FADS1 Recombinant Antibody (7A10) (V2LY-0725-LY967) in FC

Overlay Peak curve showing HepG2 cells stained with V2LY-0725-LY967 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

7A10

Application

ELISA, WB, IHC, IF, FC

Immunogen

A synthesized peptide derived from human FADS1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

fatty acid desaturase 1

Entrez Gene ID

3992

UniProt ID

O60427

Research Area

Isoform 1:
Acts as a front-end fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 5 located between a preexisting double bond and the carboxyl end of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Specifically, desaturates dihomo-gamma-linoleoate (DGLA) (20:3n-6) and eicosatetraenoate (ETA) (20:4n-3) to generate arachidonate (AA) (20:4n-6) and eicosapentaenoate (EPA) (20:5n-3), respectively (PubMed:10601301, PubMed:10769175).

As a rate limiting enzyme for DGLA (20:3n-6) and AA (20:4n-6)-derived eicosanoid biosynthesis, controls the metabolism of inflammatory lipids like prostaglandin E2, critical for efficient acute inflammatory response and maintenance of epithelium homeostasis. Contributes to membrane phospholipid biosynthesis by providing AA (20:4n-6) as a major acyl chain esterified into phospholipids. In particular, regulates phosphatidylinositol-4,5-bisphosphate levels, modulating inflammatory cytokine production in T-cells (By similarity).

Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (By similarity).

Isoform 2:
Does not exhibit any catalytic activity toward 20:3n-6, but it may enhance FADS2 activity.

Biological Process

Alpha-linolenic acid metabolic process Source: Reactome
Cell-cell signaling Source: UniProtKB
Cellular response to starvation Source: UniProtKB
Icosanoid biosynthetic process Source: UniProtKB
Linoleic acid metabolic process Source: Reactome
Long-chain fatty acid biosynthetic process Source: MGI
Phospholipid biosynthetic process Source: UniProtKB
Regulation of cell differentiation Source: UniProtKB
Regulation of transcription, DNA-templated Source: UniProtKB
Unsaturated fatty acid biosynthetic process Source: UniProtKB

Cellular Location

Isoform 1: Endoplasmic reticulum membrane; Mitochondrion
Isoform 2: Endoplasmic reticulum membrane

Topology

Cytoplasmic: 1-121
Helical: 122-142
Lumenal: 143-145
Helical: 146-170
Cytoplasmic: 171-267
Helical: 268-288
Lumenal: 289-305
Helical: 306-326
Cytoplasmic: 327-444

Zhou, C., Zhang, W., Lin, H., Zhang, L., Wu, F., Wang, Y., ... & Huang, Z. (2022). Effect of theaflavin-3, 3'-digallate on leptin-deficient induced nonalcoholic fatty liver disease might be related to lipid metabolism regulated by the Fads1/PPARδ/Fabp4 axis and gut microbiota. Frontiers in pharmacology, 13, 925264-925264.

Knez, M., Pantovic, A., Tako, E., & Boy, E. (2022). FADS1 and FADS2 as biomarkers of Zn status–a systematic review and meta-analysis. Critical Reviews in Food Science and Nutrition, 1-19.

Zhao, R., Tian, L., Zhao, B., Sun, Y., Cao, J., Chen, K., ... & Liu, M. (2020). FADS1 promotes the progression of laryngeal squamous cell carcinoma through activating AKT/mTOR signaling. Cell death & disease, 11(4), 1-14.

Reynolds, L. M., Dutta, R., Seeds, M. C., Lake, K. N., Hallmark, B., Mathias, R. A., ... & Chilton, F. H. (2020). FADS genetic and metabolomic analyses identify the∆ 5 desaturase (FADS1) step as a critical control point in the formation of biologically important lipids. Scientific reports, 10(1), 1-12.

Lian, H., Xie, P., Yin, N., Zhang, J., Zhang, X., Li, J., & Zhang, C. (2019). Linc00460 promotes osteosarcoma progression via miR-1224-5p/FADS1 axis. Life sciences, 233, 116757.

Koletzko, B., Reischl, E., Tanjung, C., Gonzalez-Casanova, I., Ramakrishnan, U., Meldrum, S., ... & Demmelmair, H. (2019). FADS1 and FADS2 polymorphisms modulate fatty acid metabolism and dietary impact on health. Annual review of nutrition, 39, 21-44.

Yuan, S., Bäck, M., Bruzelius, M., Mason, A. M., Burgess, S., & Larsson, S. (2019). Plasma phospholipid fatty acids, FADS1 and risk of 15 cardiovascular diseases: a Mendelian randomisation study. Nutrients, 11(12), 3001.

Gromovsky, A. D., Schugar, R. C., Brown, A. L., Helsley, R. N., Burrows, A. C., Ferguson, D., ... & Brown, J. M. (2018). Δ-5 fatty acid desaturase FADS1 impacts metabolic disease by balancing proinflammatory and proresolving lipid mediators. Arteriosclerosis, thrombosis, and vascular biology, 38(1), 218-231.

He, Z., Zhang, R., Jiang, F., Zhang, H., Zhao, A., Xu, B., ... & Hu, C. (2018). FADS1-FADS2 genetic polymorphisms are associated with fatty acid metabolism through changes in DNA methylation and gene expression. Clinical epigenetics, 10(1), 1-13.

Lopes-Marques, M., Kabeya, N., Qian, Y., Ruivo, R., Santos, M. M., Venkatesh, B., ... & Monroig, Ó. (2018). Retention of fatty acyl desaturase 1 (fads1) in Elopomorpha and Cyclostomata provides novel insights into the evolution of long-chain polyunsaturated fatty acid biosynthesis in vertebrates. BMC evolutionary biology, 18(1), 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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