Mouse Anti-FANCM Recombinant Antibody (F0057) (V2LY-0825-LY1381)

Name: Mouse Anti-FANCM Recombinant Antibody (F0057)
SKU: V2LY-0825-LY1381
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

F0057

Application

WB, IP, IF, IHC

Immunogen

ANCM (aa 1507-1679) made in E.coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000
IP	2-4 μg/ml lysate
IF(ICC)	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Fanconi Anemia Complementation Group M

Entrez Gene ID

57697

UniProt ID

Q8IYD8

Research Area

DNA-dependent ATPase component of the Fanconi anemia (FA) core complex (PubMed:16116422).

Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage (PubMed:16116422, PubMed:19423727, PubMed:20347428, PubMed:20347429, PubMed:29231814).

In complex with CENPS and CENPX, binds double-stranded DNA (dsDNA), fork-structured DNA (fsDNA) and Holliday junction substrates (PubMed:20347428, PubMed:20347429).

Its ATP-dependent DNA branch migration activity can process branched DNA structures such as a movable replication fork. This activity is strongly stimulated in the presence of CENPS and CENPX (PubMed:20347429).

In complex with FAAP24, efficiently binds to single-strand DNA (ssDNA), splayed-arm DNA, and 3'-flap substrates (PubMed:17289582).

In vitro, on its own, strongly binds ssDNA oligomers and weakly fsDNA, but does not bind to dsDNA (PubMed:16116434).

Biological Process

Double-strand break repair via synthesis-dependent strand annealing Source: GO_Central
Interstrand cross-link repair Source: GO_Central
Positive regulation of protein monoubiquitination Source: UniProtKB
Replication fork processing Source: UniProtKB
Replication fork reversal Source: GO_Central
Resolution of meiotic recombination intermediates Source: UniProtKB

Cellular Location

Nucleus

Involvement in disease

Spermatogenic failure 28 (SPGF28):
An autosomal recessive infertility disorder caused by spermatogenesis defects that result in oligoasthenospermia or non-obstructive azoospermia.
Premature ovarian failure 15 (POF15):
An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.

PTM

Phosphorylated; hyperphosphorylated in response to genotoxic stress.

Desjardins, S. D., Simmonds, J., Guterman, I., Kanyuka, K., Burridge, A. J., Tock, A. J., ... & Higgins, J. D. (2022). FANCM promotes class I interfering crossovers and suppresses class II non-interfering crossovers in wheat meiosis. Nature communications, 13(1), 1-12.

Panday, A., Willis, N. A., Elango, R., Menghi, F., Duffey, E. E., Liu, E. T., & Scully, R. (2021). FANCM regulates repair pathway choice at stalled replication forks. Molecular cell, 81(11), 2428-2444.

Zhang, J., Bellani, M. A., James, R. C., Pokharel, D., Zhang, Y., Reynolds, J. J., ... & Seidman, M. M. (2020). DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain. Nature communications, 11(1), 1-15.

Pan, X., Chen, Y., Biju, B., Ahmed, N., Kong, J., Goldenberg, M., ... & Zhang, D. (2019). FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops. Scientific reports, 9(1), 1-14.

Basbous, J., & Constantinou, A. (2019). A tumor suppressive DNA translocase named FANCM. Critical Reviews in Biochemistry and Molecular Biology, 54(1), 27-40.

Yin, H., Ma, H., Hussain, S., Zhang, H., Xie, X., Jiang, L., ... & Shi, Q. (2019). A homozygous FANCM frameshift pathogenic variant causes male infertility. Genetics in Medicine, 21(1), 62-70.

Domingues-Silva, B., Silva, B., & Azzalin, C. M. (2019). ALTernative functions for human FANCM at telomeres. Frontiers in Molecular Biosciences, 6, 84.

Lu, R., O’Rourke, J. J., Sobinoff, A. P., Allen, J. A., Nelson, C. B., Tomlinson, C. G., ... & Pickett, H. A. (2019). The FANCM-BLM-TOP3A-RMI complex suppresses alternative lengthening of telomeres (ALT). Nature communications, 10(1), 1-14.

Silva, B., Pentz, R., Figueira, A. M., Arora, R., Lee, Y. W., Hodson, C., ... & Azzalin, C. M. (2019). FANCM limits ALT activity by restricting telomeric replication stress induced by deregulated BLM and R-loops. Nature communications, 10(1), 1-16.

Wang, H., Li, S., Oaks, J., Ren, J., Li, L., & Wu, X. (2018). The concerted roles of FANCM and Rad52 in the protection of common fragile sites. Nature communications, 9(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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