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Rabbit Anti-FBL Monoclonal Antibody (J.99.5) (CBMAB-1255-YC)

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Basic Information

Host Animal
Rabbit
Clone
J.99.5
Application
ICC, IF, WB
Immunogen
Synthetic peptide surrounding pThr298 of human fibrillarin
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Fibrillarin
Introduction
Fibrillarin is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. Fibrillarin is located in the dense fibrillar component (DFC) of the nucleolus. Fibrillarin contains an N-terminal repetitive domain and RNA-binding domain. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
Entrez Gene ID
Human2091
Mouse14113
Rat292747
UniProt ID
HumanP22087
MouseP35550
RatP22509
Alternative Names
FIB; FLRN; Nop1; RNU3IP1
Research Area
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898).

Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:30540930).

Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity).

Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity).

Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed:32017898).

U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898).

Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239, PubMed:30540930).
Biological Process
Box C/D RNA 3'-end processing Source: GO_Central
Histone glutamine methylation Source: UniProtKB
Osteoblast differentiation Source: UniProtKB
rRNA methylation Source: UniProtKB
rRNA processing Source: ProtInc
SnoRNA localization Source: UniProtKB
Cellular Location
Nucleolus; Nucleoplasm. Fibrillar region of the nucleolus.
PTM
Ubiquitinated. Ubiquitination leads to proteasomal degradation (PubMed:19208757). Deubiquitinated by USP36 (PubMed:19208757).
By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).By similarity
Acetylated by CREBBP/CBP, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me), without affecting rRNA methylation (PubMed:30540930). Deacetylation by SIRT7 restores methylation of 'Gln-105' of histone H2A (H2AQ104me) (PubMed:30540930).

Luo, W., Lin, S., Huang, Y., Zhu, K., Zhang, F., Lin, J., ... & Liu, C. (2022). Bioinformatic Analysis and In Vitro and In Vivo Experiments Reveal That Fibrillarin Participates in the Promotion of Lung Metastasis in Hepatocellular Carcinoma. Bioengineering, 9(8), 396.

Nguyen Van Long, F., Lardy-Cleaud, A., Carène, D., Rossoni, C., Catez, F., Rollet, P., ... & Marcel, V. (2022). Low level of Fibrillarin, a ribosome biogenesis factor, is a new independent marker of poor outcome in breast cancer. BMC cancer, 22(1), 1-12.

Zhang, J., Yang, G., Li, Q., & Xie, F. (2021). Increased fibrillarin expression is associated with tumor progression and an unfavorable prognosis in hepatocellular carcinoma. Oncology Letters, 21(2), 1-1.

Wang, T., & Na, J. (2021). Fibrillarin-GFP facilitates the identification of meiotic competent oocytes. Frontiers in cell and developmental biology, 9, 648331.

Shubina, M. Y., Arifulin, E. A., Sorokin, D. V., Sosina, M. A., Tikhomirova, M. A., Serebryakova, M. V., ... & Sheval, E. V. (2020). The GAR domain integrates functions that are necessary for the proper localization of fibrillarin (FBL) inside eukaryotic cells. PeerJ, 8, e9029.

El Hassouni, B., Sarkisjan, D., Vos, J. C., Giovannetti, E., & Peters, G. J. (2019). Targeting the ribosome biogenesis key molecule fibrillarin to avoid chemoresistance. Current medicinal chemistry, 26(33), 6020-6032.

Iyer-Bierhoff, A., Krogh, N., Tessarz, P., Ruppert, T., Nielsen, H., & Grummt, I. (2018). SIRT7-dependent deacetylation of fibrillarin controls histone H2A methylation and rRNA synthesis during the cell cycle. Cell reports, 25(11), 2946-2954.

Shubina, M. Y., Musinova, Y. R., & Sheval, E. V. (2018). Proliferation, cancer, and aging—novel functions of the nucleolar methyltransferase fibrillarin?. Cell Biology International, 42(11), 1463-1466.

Bouffard, S., Dambroise, E., Brombin, A., Lempereur, S., Hatin, I., Simion, M., ... & Jamen, F. (2018). Fibrillarin is essential for S-phase progression and neuronal differentiation in zebrafish dorsal midbrain and retina. Developmental biology, 437(1), 1-16.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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