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Mouse Anti-FGG Recombinant Antibody (1E2) (CBMAB-A2958-LY)

The product is antibody recognizes FGG. The antibody 1E2 immunoassay techniques such as: WB, ELISA.
See all FGG antibodies
Published Data
Fig.1 Consecutive sections demonstrate CLSM images of immunofluorescence staining for LF (green) and FGG (red) and DAPI-induced DNA fluorescence (blue).
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Fig.2 Immunohistochemical observation of NETs and fibrin markers in thin fibrils in inflammatory exudates in acute appendicitis FGG.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1E2
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
FGG (AAH07044, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Fibrinogen Gamma Chain
Introduction
The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]
Entrez Gene ID
2266
UniProt ID
P02679
Function
Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
Biological Process
Blood coagulation, fibrin clot formation Source: UniProtKB
Cell-matrix adhesion Source: BHF-UCL
Cellular protein-containing complex assembly Source: BHF-UCL
Cellular response to interleukin-1 Source: Ensembl
Cellular response to interleukin-6 Source: Ensembl
Fibrinolysis Source: UniProtKB
Negative regulation of endothelial cell apoptotic process Source: BHF-UCL
Negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: BHF-UCL
Negative regulation of platelet aggregation Source: Ensembl
Plasminogen activation Source: UniProtKB
Platelet aggregation Source: BHF-UCL
Platelet maturation Source: Ensembl
Positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
Positive regulation of exocytosis Source: BHF-UCL
Positive regulation of heterotypic cell-cell adhesion Source: BHF-UCL
Positive regulation of peptide hormone secretion Source: BHF-UCL
Positive regulation of protein secretion Source: BHF-UCL
Positive regulation of substrate adhesion-dependent cell spreading Source: BHF-UCL
Positive regulation of vasoconstriction Source: BHF-UCL
Protein polymerization Source: BHF-UCL
Protein secretion Source: UniProtKB
Response to calcium ion Source: BHF-UCL
Cellular Location
Secreted
Involvement in disease
Congenital afibrinogenemia (CAFBN):
The disease is caused by variants affecting the gene represented in this entry. Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding. Rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen.
Dysfibrinogenemia, congenital (DYSFIBRIN):
A disorder characterized by qualitative abnormalities (dysfibrinogenemia) of the circulating fibrinogen. Affected individuals are frequently asymptomatic, but some patients have bleeding diathesis, thromboembolic complications, or both. In some cases, dysfibrinogenemia is associated with low circulating fibrinogen levels (hypodysfibrinogenemia).
PTM
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Sulfation of C-terminal tyrosines increases affinity for thrombin.

Ceznerová, E., Kaufmanová, J., Stikarová, J., Pastva, O., Loužil, J., Chrastinová, L., ... & Dyr, J. E. (2022). Thrombosis-associated hypofibrinogenemia: novel abnormal fibrinogen variant FGG c. 8G> A with oxidative posttranslational modifications. Blood Coagulation & Fibrinolysis, 33(4), 228-237.

Liu, J. X., Wang, C. J., Dai, J. H., Zhang, M. X., Lyu, B., & Jiang, B. (2022). Fibrinogen gamma-chain mutation, p. Ile171His, leads to hereditary hypofibrinogenemia. Zhonghua nei ke za zhi, 61(2), 172-176.

Zhang, H., Li, C., Song, X., Cheng, L., Liu, Q., Zhang, N., ... & Li, F. (2021). Integrated analysis reveals lung fibrinogen gamma chain as a biomarker for chronic obstructive pulmonary disease. Annals of Translational Medicine, 9(24).

Zhang, W., Gao, Z., Zeng, G., Xie, H., Liu, J., Liu, N., & Wang, G. (2020). Clinical significance of urinary plasminogen and fibrinogen gamma chain as novel potential diagnostic markers for non-small-cell lung cancer. Clinica Chimica Acta, 502, 55-65.

Simurda, T., Brunclikova, M., Asselta, R., Caccia, S., Zolkova, J., Kolkova, Z., ... & Kubisz, P. (2020). Genetic variants in the FGB and FGG genes mapping in the beta and gamma nodules of the fibrinogen molecule in congenital quantitative fibrinogen disorders associated with a thrombotic phenotype. International Journal of Molecular Sciences, 21(13), 4616.

Liu, Y. L., Yan, Z. X., Xia, Y., Xie, X. Y., Zhou, K., Xu, L. L., ... & Bi, J. W. (2020). Ligustrazine reverts anthracycline chemotherapy resistance of human breast cancer by inhibiting JAK2/STAT3 signaling and decreasing fibrinogen gamma chain (FGG) expression. American journal of cancer research, 10(3), 939.

Paulsen, B., Skille, H., Smith, E. N., Hveem, K., Gabrielsen, M. E., Brækkan, S. K., ... & Hansen, J. B. (2020). Fibrinogen gamma gene rs2066865 and risk of cancer-related venous thromboembolism. haematologica, 105(7), 1963.

Wypasek, E., Klukowska, A., Zdziarska, J., Zawilska, K., Treliński, J., Iwaniec, T., ... & Undas, A. (2019). Genetic and clinical characterization of congenital fibrinogen disorders in Polish patients: Identification of three novel fibrinogen gamma chain mutations. Thrombosis Research, 182, 133-140.

Drizlionoka, K., Zariņš, J., Ozoliņa, A., Ņikitina-Zaķe, L., & Mamaja, B. (2019). Polymorphism rs2066865 in the Fibrinogen Gamma Chain (FGG) gene increases plasma fibrinogen concentration and is associated with an increased microvascular thrombosis rate. Medicina, 55(9), 563.

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For research use only. Not intended for any clinical use.

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