Mouse Anti-FLT3 Recombinant Antibody (3H1) (CBMAB-A3041-LY)

Name: Mouse Anti-FLT3 Recombinant Antibody (3H1)
SKU: CBMAB-A3041-LY
Rating: 5 (1 reviews)

See all FLT3 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3H1

Application

IF, ELISA

Immunogen

FLT3 (NP_004110, 91 a.a. ~ 190 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Fms Related Tyrosine Kinase 3

Introduction

This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq]

Entrez Gene ID

2322

UniProt ID

P36888

Alternative Names

CD135; FLK2; STK1

Function

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

Biological Process

Animal organ regeneration Source: Ensembl
B cell differentiation Source: UniProtKB
Cellular response to cytokine stimulus Source: UniProtKB
Cellular response to glucocorticoid stimulus Source: Ensembl
Common myeloid progenitor cell proliferation Source: UniProtKB
Cytokine-mediated signaling pathway Source: UniProtKB
Dendritic cell differentiation Source: UniProtKB
Hemopoiesis Source: MGI
Leukocyte homeostasis Source: UniProtKB
Lymphocyte proliferation Source: UniProtKB
Myeloid progenitor cell differentiation Source: UniProtKB
Peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Positive regulation of MAPK cascade Source: UniProtKB
Positive regulation of MAP kinase activity Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
Positive regulation of tyrosine phosphorylation of STAT protein Source: UniProtKB
Pro-B cell differentiation Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB
Response to organonitrogen compound Source: Ensembl
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Cellular Location

Endoplasmic reticulum lumen; Membrane. Constitutively activated mutant forms with internal tandem duplications are less efficiently transported to the cell surface and a significant proportion is retained in an immature form in the endoplasmic reticulum lumen. The activated kinase is rapidly targeted for degradation.

Involvement in disease

Leukemia, acute myelogenous (AML):
The gene represented in this entry may be involved in disease pathogenesis. Somatic mutations that lead to constitutive activation of FLT3 are frequent in AML patients. These mutations fall into two classes, the most common being in-frame internal tandem duplications of variable length in the juxtamembrane region that disrupt the normal regulation of the kinase activity. Likewise, point mutations in the activation loop of the kinase domain can result in a constitutively activated kinase.
A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes.

Topology

Extracellular: 27-543
Helical: 544-563
Cytoplasmic: 564-993

PTM

N-glycosylated, contains complex N-glycans with sialic acid.
Autophosphorylated on several tyrosine residues in response to FLT3LG binding. FLT3LG binding also increases phosphorylation of mutant kinases that are constitutively activated. Dephosphorylated by PTPRJ/DEP-1, PTPN1, PTPN6/SHP-1, and to a lesser degree by PTPN12. Dephosphorylation is important for export from the endoplasmic reticulum and location at the cell membrane.
Rapidly ubiquitinated by UBE2L6 and the E3 ubiquitin-protein ligase SIAH1 after autophosphorylation, leading to its proteasomal degradation.

Elmongy, E. I., Altwaijry, N., Attallah, N. G., AlKahtani, M. M., & Henidi, H. A. (2022). In-Silico Screening of Novel Synthesized Thienopyrimidines Targeting Fms Related Receptor Tyrosine Kinase-3 and Their In-Vitro Biological Evaluation. Pharmaceuticals, 15(2), 170.

Novatcheva, E. D., Anouty, Y., Saunders, I., Mangan, J. K., & Goodman, A. M. (2022). FMS-like tyrosine kinase 3 inhibitors for the treatment of acute myeloid leukemia. Clinical Lymphoma Myeloma and Leukemia, 22(3), e161-e184.

Paiola, M., Ma, S., & Robert, J. (2022). Evolution and Potential Subfunctionalization of Duplicated fms-Related Class III Receptor Tyrosine Kinase flt3s and Their Ligands in the Allotetraploid Xenopus laevis. The Journal of Immunology, 209(5), 960-969.

Roskoski Jr, R. (2020). The role of small molecule Flt3 receptor protein-tyrosine kinase inhibitors in the treatment of Flt3-positive acute myelogenous leukemias. Pharmacological Research, 155, 104725.

Ma, W., Liang, F., Zhan, H., Jiang, X., Gao, C., Zhang, X., ... & Zhao, Z. (2020). Activated FMS‐like tyrosine kinase 3 ameliorates angiotensin II‐induced cardiac remodelling. Acta Physiologica, 230(2), e13519.

Zhong, Y., Qiu, R. Z., Sun, S. L., Zhao, C., Fan, T. Y., Chen, M., ... & Shi, Z. H. (2020). Small-molecule fms-like tyrosine kinase 3 inhibitors: an attractive and efficient method for the treatment of acute myeloid leukemia. Journal of medicinal chemistry, 63(21), 12403-12428.

Kazi, J. U., & Rönnstrand, L. (2019). FMS-like tyrosine kinase 3/FLT3: from basic science to clinical implications. Physiological reviews, 99(3), 1433-1466.

Kawase, T., Nakazawa, T., Eguchi, T., Tsuzuki, H., Ueno, Y., Amano, Y., ... & Yoshida, T. (2019). Effect of Fms-like tyrosine kinase 3 (FLT3) ligand (FL) on antitumor activity of gilteritinib, a FLT3 inhibitor, in mice xenografted with FL-overexpressing cells. Oncotarget, 10(58), 6111.

Kwon, W. S., Kim, Y. J., Ryu, D. Y., Kwon, K. J., Song, W. H., Rahman, M. S., & Pang, M. G. (2019). Fms-like tyrosine kinase 3 is a key factor of male fertility. Theriogenology, 126, 145-152.

Rivat, C., Sar, C., Mechaly, I., Leyris, J. P., Diouloufet, L., Sonrier, C., ... & Valmier, J. (2018). Inhibition of neuronal FLT3 receptor tyrosine kinase alleviates peripheral neuropathic pain in mice. Nature Communications, 9(1), 1042.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-FLT3 Recombinant Antibody (3H1)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-FLT3 Recombinant Antibody (CBXF-2413) (CAT#: CBMAB-F1978-CQ)

Mouse Anti-FLT3 (Phosphorylated Y591) Recombinant Antibody (3i15(54H1)) (CAT#: PTM-CBMAB-0381LY)

Mouse Anti-FLT3 Recombinant Antibody (CBXF-0758) (CAT#: CBMAB-F3189-CQ)

Mouse Anti-FLT3 Recombinant Antibody (CBXF-3088) (CAT#: CBMAB-F0574-CQ)

Mouse Anti-FLT3 Recombinant Antibody (CBXF-1411) (CAT#: CBMAB-F2682-CQ)

Rat Anti-FLT3 Recombinant Antibody (CBXF-3323) (CAT#: CBMAB-F0106-CQ)

Mouse Anti-FLT3 (Phosphorylated Y591) Recombinant Antibody (54H1) (CAT#: PTM-CBMAB-0413LY)

Mouse Anti-FLT3 Recombinant Antibody (CBXF-1946) (CAT#: CBMAB-F2791-CQ)

Mouse Anti-FLT3 Recombinant Antibody (4G8) (CAT#: CBMAB-C2264-LY)

Mouse Anti-FLT3 (AA 91-190) Recombinant Antibody (CBXF-3085) (CAT#: CBMAB-F3782-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry