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Rat Anti-FZD4 Monoclonal Antibody (145901) (CBMAB-1299-YC)

Provided herein is a rat monoclonal antibody against Mouse FZD4. The antibody, clone 145901, can be used for immunoassay techniques, such as IF, ICC, FC and WB.
See all FZD4 antibodies
Published Data
Fig.1 Direct binding of TNSALP and LRP6 consequently affects the phosphorylation of GSK3β.
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Fig.2 Distribution of Wnt signaling proteins in the mouse hippocampus.
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Summary

Host Animal
Rat
Specificity
Mouse, Human
Clone
145901
Antibody Isotype
IgG2A
Application
IF, ICC, FC, WB

Basic Information

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4 Phe37-Glu180
Specificity
Mouse, Human
Antibody Isotype
IgG2A
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
frizzled class receptor 4
Introduction
The frizzled gene family encodes seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. FZD4 may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway.
Entrez Gene ID
Human8322
Mouse14366
UniProt ID
HumanQ9ULV1
MouseQ61088
Alternative Names
Fz4
Function
Receptor for Wnt proteins (PubMed:30135577).

Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes (PubMed:30135577).

Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP) (By similarity).

In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs (By similarity).

A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Biological Process
Canonical Wnt signaling pathway Source: UniProtKB
Cellular response to leukemia inhibitory factor Source: Ensembl
Cellular response to retinoic acid Source: UniProtKB
Cerebellum vasculature morphogenesis Source: Ensembl
Extracellular matrix-cell signaling Source: Ensembl
Locomotion involved in locomotory behavior Source: Ensembl
Negative regulation of canonical Wnt signaling pathway Source: GO_Central
Negative regulation of cell-substrate adhesion Source: BHF-UCL
Neuron differentiation Source: UniProtKB
Non-canonical Wnt signaling pathway Source: ARUK-UCL
Norrin signaling pathway Source: BHF-UCL
Positive regulation of DNA-binding transcription factor activity Source: BHF-UCL
Positive regulation of JUN kinase activity Source: Ensembl
Positive regulation of neuron projection arborization Source: ARUK-UCL
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Progesterone secretion Source: Ensembl
Regulation of vascular endothelial growth factor receptor signaling pathway Source: Ensembl
Retinal blood vessel morphogenesis Source: Ensembl
Retina vasculature morphogenesis in camera-type eye Source: BHF-UCL
Sensory perception of sound Source: Ensembl
Substrate adhesion-dependent cell spreading Source: Ensembl
Vasculogenesis Source: Ensembl
Wnt signaling pathway Source: ARUK-UCL
Wnt signaling pathway, calcium modulating pathway Source: BHF-UCL
Cellular Location
Cell membrane
Involvement in disease
Vitreoretinopathy, exudative 1 (EVR1):
A disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. Clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease related abnormality is an arc of avascular retina in the extreme temporal periphery. In many ways the disease resembles retinopathy of prematurity but there is no evidence of prematurity or small birth weight in the patient history.
Topology
Extracellular: 37-212
Helical: 213-243
Cytoplasmic: 244-249
Helical: 250-275
Extracellular: 276-299
Helical: 300-333
Cytoplasmic: 334-336
Helical: 337-365
Extracellular: 366-383
Helical: 384-418
Cytoplasmic: 419-431
Helical: 432-460
Extracellular: 461-473
Helical: 474-495
Cytoplasmic: 496-537
PTM
Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.

Nguyen, H., Chen, H., Vuppalapaty, M., Whisler, E., Logas, K. R., Sampathkumar, P., ... & Lee, S. J. (2022). SZN-413, a FZD4 Agonist, as a Potential Novel Therapeutic for the Treatment of Diabetic Retinopathy. Translational Vision Science & Technology, 11(9), 19-19.

Geng, X., Zhao, C., Zhang, Z., Liu, Y., Zhang, X., & Ding, P. (2022). Circ_0088036 facilitates the proliferation and inflammation and inhibits the apoptosis of fibroblast-like synoviocytes through targeting miR-326/FZD4 axis in rheumatoid arthritis. Autoimmunity, 55(3), 157-167.

Wang, S., Xu, L., Zhang, Z., Wang, P., Zhang, R., He, H., & Chen, L. (2021). Overexpressed miR-375-Loaded Restrains Development of Cervical Cancer Through Down-Regulation of Frizzled Class Receptor 4 (FZD4) With Liposome Nanoparticle as a Carrier. Journal of Biomedical Nanotechnology, 17(9), 1882-1889.

Zhang, G., Liu, Z., Zhong, J., & Lin, L. (2021). Circ‐ACAP2 facilitates the progression of colorectal cancer through mediating miR‐143‐3p/FZD4 axis. European journal of clinical investigation, 51(12), e13607.

Mao, D., Wu, M., Wei, J., Zhou, X., Yang, L., & Chen, F. (2021). MicroRNA‐101a‐3p could be involved in the pathogenesis of temporomandibular joint osteoarthritis by mediating UBE2D1 and FZD4. Journal of Oral Pathology & Medicine, 50(2), 236-243.

Staropoli, P. C., Yannuzzi, N. A., Patel, N. A., Negron, C. I., Prakhunhungsit, S., & Berrocal, A. M. (2020). Novel frizzled-4 mutation is associated with familial exudative vitreoretinopathy mimicking persistent fetal vasculature. Journal of Pediatric Ophthalmology & Strabismus, 57(6), e4-e7.

Chen, L., Long, Y., Han, Z., Yuan, Z., Liu, W., Yang, F., ... & Zhong, Y. (2019). MicroRNA‑101 inhibits cell migration and invasion in bladder cancer via targeting FZD4. Experimental and therapeutic medicine, 17(2), 1476-1485.

Wang, Y., Zhang, W., Wang, Y., & Wang, S. (2018). HOXD-AS1 promotes cell proliferation, migration and invasion through miR-608/FZD4 axis in ovarian cancer. American journal of cancer research, 8(1), 170.

Yang, S., Wu, Y., Xu, T. H., de Waal, P. W., He, Y., Pu, M., ... & Xu, F. (2018). Crystal structure of the Frizzled 4 receptor in a ligand-free state. Nature, 560(7720), 666-670.

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For research use only. Not intended for any clinical use.

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