Rabbit Anti-GLUL Recombinant Antibody (D2O3F) (CBMAB-CP0840-LY)

Name: Rabbit Anti-GLUL Recombinant Antibody (D2O3F)
SKU: CBMAB-CP0840-LY
Rating: 5 (1 reviews)

See all GLUL antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D2O3F

Application

WB, IP

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala297 of human GLUL protein.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Glutamate-Ammonia Ligase

Introduction

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Entrez Gene ID

Human	2752
Mouse	14645
Rat	24957

UniProt ID

Human	P15104
Mouse	P15105
Rat	P09606

Alternative Names

Glutamate-Ammonia Ligase; Glutamate Decarboxylase; Glutamine Synthetase; EC 6.3.1.2; GLNS; GS; Glutamate-Ammonia Ligase (Glutamine Synthase); Cell Proliferation-Inducing Protein 59;

Function

Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed:30158707, PubMed:16267323).

Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity).

Essential for proliferation of fetal skin fibroblasts (PubMed:18662667).

Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed:30158707).

May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed:30158707).

Plays a role in ribosomal 40S subunit biogenesis (PubMed:26711351).

Biological Process

Ammonia assimilation cycle Source: Ensembl
Angiogenesis Source: UniProtKB-KW
Cell population proliferation Source: UniProtKB
Cellular response to starvation Source: Ensembl
Glutamate catabolic process Source: BHF-UCL
Glutamine biosynthetic process Source: GO_Central
Positive regulation of epithelial cell proliferation Source: Ensembl
Positive regulation of insulin secretion Source: Ensembl
Positive regulation of synaptic transmission, glutamatergic Source: Ensembl
Protein palmitoylation Source: UniProtKB
Regulation of endothelial cell migration Source: UniProtKB
Regulation of protein localization to nucleolus Source: UniProtKB
Regulation of sprouting angiogenesis Source: UniProtKB
Response to glucose Source: Ensembl
Ribosome biogenesis Source: UniProtKB

Cellular Location

Mitochondrion; Microsome; Cell membrane; Cytosol. Mainly localizes in the cytosol, with a fraction associated with the cell membrane.

Involvement in disease

Congenital systemic glutamine deficiency (CSGD):
Rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.

PTM

Palmitoylated; undergoes autopalmitoylation.
Ubiquitinated by ZNRF1.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-GLUL Recombinant Antibody (D2O3F)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

GLUL Matched Antibody Pair (489) (CAT#: APMAB-489LY)

Related Products

Mouse Anti-GLUL Recombinant Antibody (8G9) (CAT#: CBMAB-G3935-LY)

Mouse Anti-GLUL Recombinant Antibody (BA0405) (CAT#: CBMAB-0350CQ)

Mouse Anti-GLUL Recombinant Antibody (CBT4238) (CAT#: V2LY-0625-LY1487)

Mouse Anti-GLUL Recombinant Antibody (CBLG1-1332) (CAT#: CBMAB-G3952-LY)

Mouse Anti-GLUL Recombinant Antibody (CBLG1-1328) (CAT#: CBMAB-G3948-LY)

Mouse Anti-GLUL Recombinant Antibody (CBLG1-3189) (CAT#: CBMAB-G3937-LY)

Mouse Anti-GLUL Recombinant Antibody (CBLG1-1324) (CAT#: CBMAB-G3944-LY)

Rabbit Anti-GLUL Recombinant Antibody (BA0124) (CAT#: CBMAB-0349CQ)

Mouse Anti-GLUL Recombinant Antibody (CBLG1-2826) (CAT#: CBMAB-G0540-LY)

Mouse Anti-GLUL Recombinant Antibody (CBLG1-1327) (CAT#: CBMAB-G3947-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry