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Mouse Anti-GMNN Recombinant Antibody (1A8) (CBMAB-G4021-LY)

This product is antibody recognizes GMNN. The antibody 1A8 immunoassay techniques such as: ELISA (EL/EIA), WB.
See all GMNN antibodies
Published Data
Fig.1 Immunoprecipitation of cycling, G0/G1, S, G2/M or M/G1 HME cells with anti-Cdt1, -c-Abl, -Sp1 (negative control) and -geminin antibodies.
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Fig.2 Metaphase spread analysis of chromosome condensation in uninduced or induced Gem9 cells before or after transfection of sic-Abl or treatment with 5 µM of imatinib.
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Fig.3 The overexpressed geminin in MDA-MB-231 cells following treatment with the translational inhibitor cycloheximide (CHX) for 0-10 h with cells collected at 2 h intervals.
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Fig.4 The level of geminin and c-Abl in the nuclear or cytoplasmic fractions of the indicated cell lines.
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Fig.5 adjacent sections to those shown in (a and e) stained with geminin.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1A8
Antibody Isotype
IgG1, κ
Application
ELISA (EL/EIA), WB

Basic Information

Immunogen
Recognizes human GMNN
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
GMNN
Introduction
This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]
Entrez Gene ID
51053
UniProt ID
O75496
Alternative Names
Geminin; DNA Replication Inhibitor; Geminin; MGORS6; Gem;
Function
Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC) (PubMed:9635433, PubMed:14993212, PubMed:20129055, PubMed:24064211).

It is degraded during the mitotic phase of the cell cycle (PubMed:9635433, PubMed:14993212, PubMed:24064211).

Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle (PubMed:9635433, PubMed:14993212, PubMed:24064211).

Inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, inhibiting histone H4 acetylation and DNA replication licensing (PubMed:20129055).

Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control (PubMed:22615398).
Biological Process
Animal organ morphogenesis Source: Ensembl
DNA replication preinitiation complex assembly Source: CAFA
Negative regulation of cell cycle Source: UniProtKB
Negative regulation of DNA-dependent DNA replication Source: CAFA
Negative regulation of DNA replication Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of chromatin binding Source: CAFA
Regulation of DNA replication Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm. Mainly cytoplasmic but can be relocalized to the nucleus.
Involvement in disease
Meier-Gorlin syndrome 6 (MGORS6):
A form of Meier-Gorlin syndrome, a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal.
PTM
Phosphorylated during mitosis. Phosphorylation at Ser-184 by CK2 results in enhanced binding to Hox proteins and more potent inhibitory effect on Hox transcriptional activity.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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