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Mouse Anti-GOLGA2 Recombinant Antibody (CBFYH-0409) (CBMAB-H1279-FY)

This product is mouse antibody that recognizes GOLGA2. The antibody CBFYH-0409 can be used for immunoassay techniques such as: ELISA, WB.
See all GOLGA2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-0409
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
golgin A2
Introduction
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
Entrez Gene ID
2801
UniProt ID
Q08379
Alternative Names
Golgin A2; Golgi Autoantigen, Golgin Subfamily A, 2; 130 KDa Cis-Golgi Matrix Protein; Golgi Matrix Protein GM130; GM130 Autoantigen; SY11 Protein; Golgin-95; GM130; Golgin Subfamily A Member 2
Function
Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) (PubMed:16489344).

Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity).

Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity).

Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940).

Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940).

Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity).

Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421).

Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).
Biological Process
Asymmetric cell division Source: UniProtKB
Centrosome cycle Source: UniProtKB
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: UniProtKB
Golgi disassembly Source: UniProtKB
Golgi organization Source: GO_Central
Golgi ribbon formation Source: UniProtKB
Meiotic spindle assembly Source: UniProtKB
Microtubule nucleation Source: UniProtKB
Mitotic spindle assembly Source: UniProtKB
Negative regulation of autophagy Source: ParkinsonsUK-UCL
Negative regulation of protein binding Source: UniProtKB
Positive regulation of protein glycosylation Source: ParkinsonsUK-UCL
Protein glycosylation Source: UniProtKB
Protein homotetramerization Source: UniProtKB
Protein transport Source: UniProtKB-KW
Spindle assembly Source: UniProtKB
Cellular Location
Spindle pole; Cis-Golgi network membrane; Endoplasmic reticulum-Golgi intermediate compartment membrane. Associates with the mitotic spindle during mitosis (PubMed:26165940).
PTM
Cleaved by caspases at the onset of apoptosis.
Methylation by PRMT5 is required for Golgi ribbon formation. While dimethylation at Arg-30 and Arg-35 are confirmed in vivo, it is unclear whether Arg-18 is methylated in vivo.
Phosphorylated at Ser-37 by CDK1 at the onset of mitosis, inhibiting the interaction with p115/USO1 and triggering Golgi disassembly (PubMed:20421892, PubMed:26165940). Phosphorylated at Ser-37 in prophase as the Golgi complex starts to break down, and remains phosphorylated during further breakdown and partitioning of the Golgi fragments in metaphase and anaphase. In telophase, GM130 is dephosphorylated by PP2A as the Golgi fragments start to reassemble (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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