Mouse Anti-GOLIM4 Recombinant Antibody (A1/118) (CBMAB-G4303-LY)

Name: Mouse Anti-GOLIM4 Recombinant Antibody (A1/118)
SKU: CBMAB-G4303-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A1/118

Application

ELISA, FC

Immunogen

GPP130 (Golgi phosphoprotein of 130kDa) from Vero monkey cells

Specificity

Monkey, Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

lyophilized

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

golgi integral membrane protein 4

Introduction

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi-resident protein. It may process proteins synthesized in the rough endoplasmic reticulum and assist in the transport of protein cargo through the Golgi apparatus.

Entrez Gene ID

Human	27333
Monkey	713937

UniProt ID

Human	O00461
Monkey	A0A0D9RJB0

Function

Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.

Cellular Location

Golgi stack membrane; Endosome membrane; Membrane. Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome.

Topology

Cytoplasmic: 2-12
Helical: 13-33
Lumenal: 34-696

PTM

Phosphorylated probably by c-AMP-dependent kinases in its lumenal part.
O-glycosylated; modified by sialic acid residues.
N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues.

Kan, B., Yan, G., Shao, Y., Zhang, Z., & Xue, H. (2022). CircRNA RNF10 inhibits tumorigenicity by targeting miR‐942‐5p/GOLIM4 axis in breast cancer. Environmental and Molecular Mutagenesis, 63(7), 362-372.

Luo, C. L., Xu, X. C., Liu, C. J., He, S., Chen, J. R., Feng, Y. C., ... & Bei, J. X. (2021). RBFOX2/GOLIM4 splicing Axis activates vesicular transport pathway to promote nasopharyngeal carcinogenesis. Advanced Science, 8(16), 2004852.

Lin, B., Liu, C., Shi, E., Jin, Q., Zhao, W., Wang, J., & Ji, R. (2021). MiR-105-3p acts as an oncogene to promote the proliferation and metastasis of breast cancer cells by targeting GOLIM4. BMC cancer, 21, 1-10.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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