Mouse Anti-GPD1L Recombinant Antibody (14E2) (CBMAB-G0691-LY)

This product is antibody recognizes GPD1L. The antibody 14E2 immunoassay techniques such as: WB.
See all GPD1L antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

14E2

Antibody Isotype

IgG1, κ

Application

Basic Information

Immunogen

Recombinant human GPD1L (1-351aa) purified from E. coli

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

glycerol-3-phosphate dehydrogenase 1-like

Introduction

The protein encoded by this gene catalyzes the conversion of sn-glycerol 3-phosphate to glycerone phosphate. The encoded protein is found in the cytoplasm, associated with the plasma membrane, where it binds the sodium channel, voltage-gated, type V, alpha subunit (SCN5A). Defects in this gene are a cause of Brugada syndrome type 2 (BRS2) as well as sudden infant death syndrome (SIDS). [provided by RefSeq, Jul 2010]

Entrez Gene ID

23171

UniProt ID

Q8N335

Alternative Names

Glycerol-3-Phosphate Dehydrogenase 1 Like; EC 1.1.1.8; GPD1-L; Glycerol-3-Phosphate Dehydrogenase 1-Like Protein; KIAA0089; EC 1.1.1;

Function

Plays a role in regulating cardiac sodium current; decreased enzymatic activity with resulting increased levels of glycerol 3-phosphate activating the DPD1L-dependent SCN5A phosphorylation pathway, may ultimately lead to decreased sodium current; cardiac sodium current may also be reduced due to alterations of NAD(H) balance induced by DPD1L.

Biological Process

Carbohydrate metabolic process Source: InterPro
Glycerol-3-phosphate catabolic process Source: InterPro
NADH metabolic process Source: Ensembl
NAD metabolic process Source: BHF-UCL
Negative regulation of peptidyl-serine phosphorylation Source: BHF-UCL
Negative regulation of protein kinase C signaling Source: BHF-UCL
Positive regulation of protein localization to cell surface Source: BHF-UCL
Positive regulation of sodium ion transport Source: BHF-UCL
Regulation of heart rate Source: BHF-UCL
Regulation of sodium ion transmembrane transporter activity Source: BHF-UCL
Regulation of ventricular cardiac muscle cell membrane depolarization Source: BHF-UCL
Ventricular cardiac muscle cell action potential Source: BHF-UCL

Cellular Location

Cytoplasm. Localized to the region of the plasma membrane.

Involvement in disease

Brugada syndrome 2 (BRGDA2):
A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs, the individual will faint and may die in a few minutes if the heart is not reset.

References

Usuda, K., Hayashi, K., Ishikawa, T., Aizawa, Y., Kato, T., Kusayama, T., ... & Takamura, M. (2021). Novel variant of the glycerol-3-phosphate dehydrogenase-1 Like (GPD1-L) gene in Japanese Brugada syndrome patients. European Heart Journal, 42(Supplement_1), ehab724-0640.

Yuan, M., Guo, Y., Xia, H., Xu, H., Deng, H., & Yuan, L. (2021). Novel SCN5A and GPD1L variants identified in two unrelated han-Chinese patients with clinically suspected brugada syndrome. Frontiers in Cardiovascular Medicine, 8, 758903.

Liu, H., Wang, S., Cheng, A., Han, Z., Feng, Z., & Guo, C. (2021). GPD1L is negatively associated with HIF1α expression and predicts lymph node metastasis in oral and HPV‐Oropharyngeal cancer. Oral Diseases, 27(7), 1654-1666.

Wang, Z., Hao, J., & Chen, D. (2019). Long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1) regulates proliferation, apoptosis, and inflammation of chondrocytes via the miR-181a/glycerol-3-phosphate dehydrogenase 1-like (GPD1L) axis. Medical science monitor: international medical journal of experimental and clinical research, 25, 8084.

Huang, H., Chen, Y. Q., Fan, L. L., Guo, S., Li, J. J., Jin, J. Y., & Xiang, R. (2018). Whole‐exome sequencing identifies a novel mutation of GPD1L (R189X) associated with familial conduction disease and sudden death. Journal of Cellular and Molecular Medicine, 22(2), 1350-1354.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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