Mouse Anti-GPIHBP1 Recombinant Antibody (1F9) (CBMAB-G4599-LY)

Name: Mouse Anti-GPIHBP1 Recombinant Antibody (1F9)
SKU: CBMAB-G4599-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1F9

Application

ELISA, WB

Immunogen

Full-length recombinant protein corresponding to aa19184 from human GPIHBP1 with a GST Tag. MW of the GST tag alone is 26kD. (NP_835466.1)

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1

Introduction

This gene encodes a capillary endothelial cell protein that facilitates the lipolytic processing of triglyceride-rich lipoproteins. The encoded protein is a glycosylphosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly6) family. This protein plays a major role in transporting lipoprotein lipase (LPL) from the subendothelial spaces to the capillary lumen. Mutations in this gene are the cause of hyperlipoproteinemia, type 1D. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Entrez Gene ID

338328

UniProt ID

Q8IV16

Alternative Names

Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1; GPI Anchored High Density Lipoprotein Binding Protein 1; GPI-Anchored HDL-Binding Protein 1; Endothelial Cell LPL Transporter; GPI-HBP1; Glycosylphosphatidylinositol-Anchored High Density Lipoprotein-Binding Protein 1; High Density Lipoprotein-Binding Protein 1; HYPL1D; HBP1;

Function

Mediates the transport of lipoprotein lipase LPL from the basolateral to the apical surface of endothelial cells in capillaries (By similarity).

Anchors LPL on the surface of endothelial cells in the lumen of blood capillaries (By similarity).

Protects LPL against loss of activity, and against ANGPTL4-mediated unfolding (PubMed:27929370, PubMed:29899144).

Thereby, plays an important role in lipolytic processing of chylomicrons by LPL, triglyceride metabolism and lipid homeostasis (PubMed:19304573, PubMed:21314738).

Binds chylomicrons and phospholipid particles that contain APOA5 (PubMed:17997385, PubMed:19304573).

Binds high-density lipoprotein (HDL) and plays a role in the uptake of lipids from HDL (By similarity).

Biological Process

Cholesterol homeostasis Source: BHF-UCL
Intracellular protein transport Source: BHF-UCL
Positive regulation of chylomicron remnant clearance Source: BHF-UCL
Positive regulation of lipoprotein lipase activity Source: ARUK-UCL
Protein import Source: BHF-UCL
Protein localization to cell surface Source: BHF-UCL
Protein stabilization Source: ARUK-UCL
Response to heparin Source: BHF-UCL
Transcytosis Source: BHF-UCL
Triglyceride homeostasis Source: BHF-UCL

Cellular Location

Apical cell membrane; Basolateral cell membrane; Cell membrane

Involvement in disease

Hyperlipoproteinemia 1D (HLPP1D):
An autosomal recessive disorder characterized by hyperlipoproteinemia, decreased plasma LPL levels in some patients, high plasma triglyceride levels, and refractory fasting chylomicronemia.

PTM

Glycosylation of Asn-78 is critical for cell surface localization.By similarity
Sulfation of a Tyr in the N-terminal acidic region increases the affinity for LPL.

Young, S. G., Song, W., Yang, Y., Birrane, G., Jiang, H., Beigneux, A. P., ... & Fong, L. G. (2022). A protein of capillary endothelial cells, GPIHBP1, is crucial for plasma triglyceride metabolism. Proceedings of the National Academy of Sciences, 119(36), e2211136119.

Kristensen, K. K., Leth-Espensen, K. Z., Kumari, A., Grønnemose, A. L., Lund-Winther, A. M., Young, S. G., & Ploug, M. (2021). GPIHBP1 and ANGPTL4 utilize protein disorder to orchestrate order in plasma triglyceride metabolism and regulate compartmentalization of LPL activity. Frontiers in Cell and Developmental Biology, 9, 702508.

Miyashita, K., Lutz, J., Hudgins, L. C., Toib, D., Ashraf, A. P., Song, W., ... & Beigneux, A. P. (2020). Chylomicronemia from GPIHBP1 autoantibodies. Journal of Lipid Research, 61(11), 1365-1376.

Eguchi, J., Miyashita, K., Fukamachi, I., Nakajima, K., Murakami, M., Kawahara, Y., ... & Beigneux, A. P. (2019). GPIHBP1 autoantibody syndrome during interferon β1a treatment. Journal of clinical lipidology, 13(1), 62-69.

Young, S. G., Fong, L. G., Beigneux, A. P., Allan, C. M., He, C., Jiang, H., ... & Ploug, M. (2019). GPIHBP1 and lipoprotein lipase, partners in plasma triglyceride metabolism. Cell metabolism, 30(1), 51-65.

Arora, R., Nimonkar, A. V., Baird, D., Wang, C., Chiu, C. H., Horton, P. A., ... & Trauger, J. W. (2019). Structure of lipoprotein lipase in complex with GPIHBP1. Proceedings of the National Academy of Sciences, 116(21), 10360-10365.

Birrane, G., Beigneux, A. P., Dwyer, B., Strack-Logue, B., Kristensen, K. K., Francone, O. L., ... & Meiyappan, M. (2019). Structure of the lipoprotein lipase–GPIHBP1 complex that mediates plasma triglyceride hydrolysis. Proceedings of the National Academy of Sciences, 116(5), 1723-1732.

Kristensen, K. K., Midtgaard, S. R., Mysling, S., Kovrov, O., Hansen, L. B., Skar-Gislinge, N., ... & Ploug, M. (2018). A disordered acidic domain in GPIHBP1 harboring a sulfated tyrosine regulates lipoprotein lipase. Proceedings of the National Academy of Sciences, 115(26), E6020-E6029.

Miyashita, K., Fukamachi, I., Nagao, M., Ishida, T., Kobayashi, J., Machida, T., ... & Nakajima, K. (2018). An enzyme-linked immunosorbent assay for measuring GPIHBP1 levels in human plasma or serum. Journal of clinical lipidology, 12(1), 203-210.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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