Rabbit Anti-GPR25 Recombinant Antibody (EG1398) (CBMAB-EN1677-LY)

The product is antibody recognizes GPR25. The antibody EG1398 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:1000.
See all GPR25 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

EG1398

Antibody Isotype

IgG

Application

WB: 1:500~1:1000 ELISA: 1:1000

Basic Information

Immunogen

The antibody was produced against synthesized peptide derived from internal of human GPR25.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

G Protein-Coupled Receptor 25

Introduction

This gene is intronless and encodes a member of the G-protein coupled receptor 1 family. G-protein coupled receptors are membrane proteins which activate signaling cascades as a response to extracellular stress. This gene has been linked to arterial stiffness. [provided by RefSeq, Nov 2012]

Entrez Gene ID

2848

UniProt ID

O00155

Alternative Names

G Protein-Coupled Receptor 25;

Function

Orphan receptor.

Biological Process

G protein-coupled receptor signaling pathway Source: GO_Central

Cellular Location

Cell membrane

Topology

Extracellular: 1-39
Helical: 40-60
Cytoplasmic: 61-75
Helical: 76-96
Extracellular: 97-126
Helical: 127-147
Cytoplasmic: 148-155
Helical: 156-176
Extracellular: 177-200
Helical: 201-220
Cytoplasmic: 221-242
Helical: 243-263
Extracellular: 264-289
Helical: 290-310
Cytoplasmic: 311-361

References

Yamanouchi, J., Ikeda, Y., Hato, T., Yasukawa, M., & Takenaka, K. (2018). A Heterozygous Mutation of G-Protein-Coupled Receptor 25 in a Family with Inherited Thrombocytopenia and Thrombosis.

Zhang, J., Wan, Y., Fang, C., Chen, J., Ouyang, W., Li, J., & Wang, Y. (2018). The orphan G protein-coupled receptor 25 (GPR25) is activated by Apelin and Apela in non-mammalian vertebrates. Biochemical and Biophysical Research Communications, 501(2), 408-414.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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