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Mouse Anti-GRIN2B Recombinant Antibody (NR2B) (CBMAB-N0864-WJ)

This product is a Mouse antibody that recognizes GRIN2B. The antibody NR2B can be used for immunoassay techniques such as: ICC, IHC-P, IP, WB.
See all GRIN2B antibodies
Published Data
Fig.1 Western blotting analysis of NR2A and NR2B in tissues resected from the ATL and the hippocampal regions. Blots show a single band at the predicted size of ~177 kDa for NR2A, ~166 kDa for NR2B and ~37 kDa for GAPDH.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
NR2B
Antibody Isotype
IgG1
Application
ICC, IHC-P, IP, WB

Basic Information

Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 1 mg/ml BSA
Preservative
0.05 % sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Glutamate Ionotropic Receptor NMDA Type Subunit 2B
Introduction
This gene encodes a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. The encoded protein is a subunit of the NMDA receptor ion channel which acts as an agonist binding site for glutamate. The NMDA receptors mediate a slow calcium-permeable component of excitatory synaptic transmission in the central nervous system. The NMDA receptors are heterotetramers of seven genetically encoded, differentially expressed subunits including NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The early expression of this gene in development suggests a role in brain development, circuit formation, synaptic plasticity, and cellular migration and differentiation. Naturally occurring mutations within this gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia. [provided by RefSeq, Aug 2017]
Entrez Gene ID
Human2904
Mouse14812
Rat24410
UniProt ID
HumanQ13224
MouseQ01097
RatQ00960
Alternative Names
Glutamate Ionotropic Receptor NMDA Type Subunit 2B; Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B; Glutamate [NMDA] Receptor Subunit Epsilon-2; N-Methyl D-Aspartate Receptor Subtype 2B; N-Methyl-D-Aspartate Receptor Subunit 3; NMDAR2B; GluN2B; NR2B;
Function
Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+ (PubMed:8768735, PubMed:26919761, PubMed:26875626, PubMed:28126851).

Sensitivity to glutamate and channel kinetics depend on the subunit composition (PubMed:8768735, PubMed:26875626).

In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Contributes to neural pattern formation in the developing brain. Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity (By similarity).
Biological Process
Brain development Source: ARUK-UCL
Calcium ion transmembrane import into cytosol Source: UniProtKB
Chemical synaptic transmission Source: ProtInc
Excitatory chemical synaptic transmission Source: ARUK-UCL
Excitatory postsynaptic potential Source: GO_Central
Glutamate receptor signaling pathway Source: ProtInc
Learning or memory Source: ARUK-UCL
Long-term synaptic potentiation Source: GO_Central
Multicellular organism development Source: ARUK-UCL
Negative regulation of dendritic spine maintenance Source: ARUK-UCL
Positive regulation of cysteine-type endopeptidase activity Source: ARUK-UCL
Positive regulation of neuron death Source: ARUK-UCL
Protein heterotetramerization Source: UniProtKB
Regulation of synaptic plasticity Source: ARUK-UCL
Response to ethanol Source: UniProtKB
Cellular Location
Late endosome; Cell membrane; Postsynaptic cell membrane; Cytoskeleton; Lysosome. Co-localizes with the motor protein KIF17 along microtubules.
Involvement in disease
Mental retardation, autosomal dominant 6, with or without seizures (MRD6):
A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRD6 additional features may include seizures, hypotonia, abnormal movements, such as dystonia, and autistic features.
Developmental and epileptic encephalopathy 27 (DEE27):
A form of epileptic encephalopathy, a heterogeneous group of severe early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent.
A chromosomal aberrations involving GRIN2B has been found in patients with mental retardation. Translocations t(9;12)(p23;p13.1) and t(10;12)(q21.1;p13.1) with a common breakpoint in 12p13.1.
Topology
Extracellular: 27-557
Helical: 558-576
Cytoplasmic: 577-603
Discontinuously helical: 604-623
Cytoplasmic: 624-630
Helical: 631-646
Extracellular: 647-817
Helical: 818-837
Cytoplasmic: 838-1484
PTM
Phosphorylated on tyrosine residues (By similarity). Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity (By similarity).

Sabo, S. L., Lahr, J. M., Offer, M., Weekes, A. L., & Sceniak, M. P. (2022). GRIN2B-related neurodevelopmental disorder: Current understanding of pathophysiological mechanisms. Frontiers in Synaptic Neuroscience, 14.

Shah, A. A., Amjad, M., Hassan, J. U., Ullah, A., Mahmood, A., Deng, H., ... & Xia, K. (2022). Molecular insights into the role of pathogenic nssnps in grin2b gene provoking neurodevelopmental disorders. Genes, 13(8), 1332.

Engdahl, E., Alavian-Ghavanini, A., Forsell, Y., Lavebratt, C., & Rüegg, J. (2021). Childhood adversity increases methylation in the GRIN2B gene. Journal of psychiatric research, 132, 38-43.

Poltavskaya, E. G., Fedorenko, O. Y., Kornetova, E. G., Loonen, A. J., Kornetov, A. N., Bokhan, N. A., & Ivanova, S. A. (2021). Study of early onset schizophrenia: Associations of GRIN2A and GRIN2B polymorphisms. Life, 11(10), 997.

Kim, J. I., Kim, J. W., Park, S., Hong, S. B., Lee, D. S., Paek, S. H., ... & Kim, B. N. (2020). The GRIN2B and GRIN2A gene variants are associated with continuous performance test variables in ADHD. Journal of Attention Disorders, 24(11), 1538-1546.

Soto, D., Olivella, M., Grau, C., Armstrong, J., Alcon, C., Gasull, X., ... & Altafaj, X. (2019). L-Serine dietary supplementation is associated with clinical improvement of loss-of-function GRIN2B-related pediatric encephalopathy. Science signaling, 12(586), eaaw0936.

Myers, S. J., Yuan, H., Kang, J. Q., Tan, F. C. K., Traynelis, S. F., & Low, C. M. (2019). Distinct roles of GRIN2A and GRIN2B variants in neurological conditions. F1000Research, 8.

Fachim, H. A., Loureiro, C. M., Corsi-Zuelli, F., Shuhama, R., Louzada-Junior, P., Menezes, P. R., ... & Reynolds, G. P. (2019). GRIN2B promoter methylation deficits in early-onset schizophrenia and its association with cognitive function. Epigenomics, 11(4), 401-410.

Bell, S., Maussion, G., Jefri, M., Peng, H., Theroux, J. F., Silveira, H., ... & Ernst, C. (2018). Disruption of GRIN2B impairs differentiation in human neurons. Stem Cell Reports, 11(1), 183-196.

Alavian-Ghavanini, A., Lin, P. I., Lind, P. M., Risén Rimfors, S., Halin Lejonklou, M., Dunder, L., ... & Rüegg, J. (2018). Prenatal bisphenol A exposure is linked to epigenetic changes in glutamate receptor subunit gene Grin2b in female rats and humans. Scientific reports, 8(1), 11315.

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For research use only. Not intended for any clinical use.

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