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Mouse Anti-GSDME Recombinant Antibody (1E10) (CBMAB-A2193-LY)

The product is antibody recognizes DFNA5. The antibody 1E10 immunoassay techniques such as: WB, ELISA.
See all GSDME antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1E10
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
DFNA5 (NP_004394.1, 111 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Gasdermin E
Introduction
Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
Entrez Gene ID
1687
UniProt ID
O60443
Alternative Names
ICERE-1
Function
Gasdermin-E:
Precursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis (PubMed:27281216, PubMed:28459430).

This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-E, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed:28459430).

Gasdermin-E, N-terminal:
Pore-forming protein produced by cleavage by CASP3 or granzyme B (GZMB), which converts non-inflammatory apoptosis to pyroptosis or promotes granzyme-mediated pyroptosis, respectively (PubMed:27281216, PubMed:28459430, PubMed:32188940).

After cleavage, moves to the plasma membrane, homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis (PubMed:28459430, PubMed:32188940).

Binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate (PubMed:28459430).

Cleavage by CASP3 switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis (PubMed:27281216, PubMed:28459430, PubMed:32188940).

Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents (PubMed:28045099).

Exhibits bactericidal activity (PubMed:27281216).

Cleavage by GZMB promotes tumor suppressor activity by triggering robust anti-tumor immunity (PubMed:21522185, PubMed:32188940).

Suppresses tumors by mediating granzyme-mediated pyroptosis in target cells of natural killer (NK) cells: cleavage by granzyme B (GZMB), delivered to target cells from NK-cells, triggers pyroptosis of tumor cells and tumor suppression (PubMed:32188940, PubMed:31953257).

May play a role in the p53/TP53-regulated cellular response to DNA damage (PubMed:16897187).
Biological Process
Cell death Source: UniProtKB
Cellular response to tumor necrosis factor Source: UniProtKB
Cellular response to virus Source: UniProtKB
Granzyme-mediated programmed cell death signaling pathway Source: UniProtKB
Inner ear receptor cell differentiation Source: Ensembl
Necrotic cell death Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Positive regulation of immune response to tumor cell Source: UniProtKB
Positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of MAPK cascade Source: UniProtKB
Pyroptosis Source: UniProtKB
Sensory perception of sound Source: ProtInc
Cellular Location
Gasdermin-E, N-terminal: Cell membrane
Gasdermin-E: Cytosol
Involvement in disease
Deafness, autosomal dominant, 5 (DFNA5):
A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
PTM
Cleavage at Asp-270 by CASP3 (mature and uncleaved precursor forms) or granzyme B (GZMB) relieves autoinhibition and is sufficient to initiate pyroptosis.
Gasdermin-E:
Succination by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits processing by caspases, and ability to initiate pyroptosis (PubMed:32820063). Succination modification is catalyzed by a non-enzymatic reaction caused by an accumulation of fumarate (PubMed:32820063).

Wu, J., Lin, S., Chen, W., Lian, G., Wu, W., Chen, A., ... & Xie, L. (2023). TNF-α contributes to sarcopenia through caspase-8/caspase-3/GSDME-mediated pyroptosis. Cell Death Discovery, 9(1), 76.

Bhat, A. A., Thapa, R., Afzal, O., Agrawal, N., Almalki, W. H., Kazmi, I., ... & Gupta, G. (2023). The pyroptotic role of Caspase-3/GSDME signalling pathway among various cancer: A Review. International Journal of Biological Macromolecules, 124832.

De Schutter, E., Croes, L., Ibrahim, J., Pauwels, P., Op de Beeck, K., Vandenabeele, P., & Van Camp, G. (2021). GSDME and its role in cancer: From behind the scenes to the front of the stage. International Journal of Cancer, 148(12), 2872-2883.

Shen, X., Wang, H., Weng, C., Jiang, H., & Chen, J. (2021). Caspase 3/GSDME-dependent pyroptosis contributes to chemotherapy drug-induced nephrotoxicity. Cell death & disease, 12(2), 186.

Jiang, M., Qi, L., Li, L., & Li, Y. (2020). The caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer. Cell death discovery, 6(1), 112.

Hu, L., Chen, M., Chen, X., Zhao, C., Fang, Z., Wang, H., & Dai, H. (2020). Chemotherapy-induced pyroptosis is mediated by BAK/BAX-caspase-3-GSDME pathway and inhibited by 2-bromopalmitate. Cell death & disease, 11(4), 281.

Zhang, Y., Yang, J., Wen, Z., Chen, X., Yu, J., Yuan, D., ... & Zhu, J. (2020). A novel 3’, 5’-diprenylated chalcone induces concurrent apoptosis and GSDME-dependent pyroptosis through activating PKCδ/JNK signal in prostate cancer. Aging (Albany NY), 12(10), 9103.

Deng, B. B., Jiao, B. P., Liu, Y. J., Li, Y. R., & Wang, G. J. (2020). BIX‐01294 enhanced chemotherapy effect in gastric cancer by inducing GSDME‐mediated pyroptosis. Cell Biology International, 44(9), 1890-1899.

Zhang, C. C., Li, C. G., Wang, Y. F., Xu, L. H., He, X. H., Zeng, Q. Z., ... & Ouyang, D. Y. (2019). Chemotherapeutic paclitaxel and cisplatin differentially induce pyroptosis in A549 lung cancer cells via caspase-3/GSDME activation. Apoptosis, 24, 312-325.

Mai, F. Y., He, P., Ye, J. Z., Xu, L. H., Ouyang, D. Y., Li, C. G., ... & Hu, B. (2019). Caspase‐3‐mediated GSDME activation contributes to cisplatin‐and doxorubicin‐induced secondary necrosis in mouse macrophages. Cell Proliferation, 52(5), e12663.

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For research use only. Not intended for any clinical use.

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