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Mouse Anti-H2BC1 Recombinant Antibody (A139) (CBMAB-AP9649LY)

The product is antibody recognizes H2BC1. The antibody A139 immunoassay techniques such as: WB.
See all H2BC1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Monkey Zebrafish, Mouse, Rat
Clone
A139
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
A synthetic peptide of human HIST1H2BA
Specificity
Human, Monkey Zebrafish, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
Affinity purity
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
H2B clustered histone 1
Entrez Gene ID
Human255626
Mouse319177
Rat24829
Zebrafish100333496
UniProt ID
HumanQ96A08
MouseP70696
RatQ00729
MonkeyA0A0D9SCM2
ZebrafishX1WG17
Alternative Names
STBP, H2BFU, TSH2B, bA317E16.3,
Function
Variant histone specifically required to direct the transformation of dissociating nucleosomes to protamine in male germ cells (By similarity).

Entirely replaces classical histone H2B prior nucleosome to protamine transition and probably acts as a nucleosome dissociating factor that creates a more dynamic chromatin, facilitating the large-scale exchange of histones (By similarity).

Core component of nucleosome (By similarity).

Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template (By similarity).

Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability (By similarity).

DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling (By similarity).

Also found in fat cells, its function and the presence of post-translational modifications specific to such cells are still unclear (PubMed:21249133).
Biological Process
Nucleosome assembly Source: UniProtKB
Nucleosome disassembly Source: UniProtKB
Spermatogenesis, exchange of chromosomal proteins Source: UniProtKB
Cellular Location
Nucleus; Chromosome
PTM
Monoubiquitination at Lys-36 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-122 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.
Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
Acetylated during spermatogenesis. Acetylated form is most abundant in spermatogonia compared to spermatocytes and round spermatids (By similarity).
Phosphorylated at Thr-117 in spermatogonia, spermatocytes and round spermatids.
Methylated at Lys-118 in spermatogonia, spermatocytes and round spermatids.
Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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