Mouse Anti-HCN4 (AA 1-1198 ) Recombinant Antibody (CBFYH-0787) (CBMAB-H0273-FY)

Name: Mouse Anti-HCN4 (AA 1-1198 ) Recombinant Antibody (CBFYH-0787)
SKU: CBMAB-H0273-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-0787

Application

WB, IHC, IF, AM

Immunogen

Fusion protein amino acids 1-1198 of rat HCN4

Specificity

Human, Mouse, Rat, Rabbit

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-1198

More Infomation

Target

Full Name

Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4

Introduction

This gene encodes a member of the hyperpolarization-activated cyclic nucleotide-gated potassium channels. The encoded protein shows slow kinetics of activation and inactivation, and is necessary for the cardiac pacemaking process. This channel may also mediate responses to sour stimuli. Mutations in this gene have been linked to sick sinus syndrome 2, also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. Two pseudogenes have been identified on chromosome 15.

Entrez Gene ID

Human	10021
Mouse	330953
Rat	59266
Rabbit	100009452

UniProt ID

Human	Q9Y3Q4
Mouse	O70507
Rat	Q9JKA7
Rabbit	Q9TV66

Alternative Names

Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4; Potassium/Sodium Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 4; Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4; Hyperpolarization Activated Cyclic Nucleotide-Gated Cation Channel 4; SSS2

Function

Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) that regulate the rhythm of heart beat. May contribute to the native pacemaker currents in neurons (Ih). May mediate responses to sour stimuli.

Biological Process

Blood circulation Source: ProtInc
Cardiac conduction system development Source: BHF-UCL
Cation transport Source: BHF-UCL
Cellular response to cAMP Source: UniProtKB
Cellular response to cGMP Source: UniProtKB
Membrane depolarization during cardiac muscle cell action potential Source: BHF-UCL
Membrane depolarization during SA node cell action potential Source: BHF-UCL
Muscle contraction Source: ProtInc
Potassium ion import across plasma membrane Source: BHF-UCL
Potassium ion transmembrane transport Source: UniProtKB
Regulation of cardiac muscle cell action potential involved in regulation of contraction Source: BHF-UCL
Regulation of cardiac muscle contraction Source: BHF-UCL
Regulation of heart rate Source: UniProtKB
Regulation of heart rate by cardiac conduction Source: BHF-UCL
Regulation of ion transmembrane transport Source: UniProtKB-KW
Regulation of membrane depolarization Source: BHF-UCL
Regulation of membrane potential Source: UniProtKB
SA node cell action potential Source: BHF-UCL
Sinoatrial node development Source: BHF-UCL
Sodium ion import across plasma membrane Source: BHF-UCL
Sodium ion transmembrane transport Source: UniProtKB

Cellular Location

Cell membrane

Involvement in disease

Sick sinus syndrome 2 (SSS2):
The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors. SSS2 onset is in utero or at birth.
Brugada syndrome 8 (BRGDA8):
A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs, the individual will faint and may die in a few minutes if the heart is not reset.

Topology

Cytoplasmic: 1-266
Helical: 267-287
Extracellular: 288-293
Helical: 294-314
Cytoplasmic: 315-340
Helical: 341-361
Extracellular: 362-368
Helical: 369-389
Cytoplasmic: 390-420
Helical: 421-441
Extracellular: 442-464
Pore-forming: 465-486
Extracellular: 487-496
Helical: 497-517
Cytoplasmic: 518-1203

Shi, L., Shen, J., Jin, X., Li, Z., Ma, J., Huang, X., ... & Pan, Z. (2021). Deficiency of CXXC finger protein 1 leads to small changes in heart rate but moderate epigenetic alterations and significant protein downregulation of hyperpolarization-activated cyclic nucleotide-gated 4 (HCN4) ion channels in mice. Heart Rhythm, 18(10), 1780-1789.

Kharouf, Q., Phillips, A. M., Bleakley, L. E., Morrisroe, E., Oyrer, J., Jia, L., ... & Reid, C. A. (2020). The hyperpolarization‐activated cyclic nucleotide‐gated 4 channel as a potential anti‐seizure drug target. British journal of pharmacology, 177(16), 3712-3729.

Romanelli, M. N., Del Lungo, M., Guandalini, L., Zobeiri, M., Gyökeres, A., Árpádffy-Lovas, T., ... & Cerbai, E. (2019). EC18 as a tool to understand the role of HCN4 channels in mediating hyperpolarization-activated current in tissues. ACS Medicinal Chemistry Letters, 10(4), 584-589.

Akimoto, M., VanSchouwen, B., & Melacini, G. (2018). The structure of the apo cAMP‐binding domain of HCN 4–a stepping stone toward understanding the cAMP‐dependent modulation of the hyperpolarization‐activated cyclic‐nucleotide‐gated ion channels. The FEBS Journal, 285(12), 2182-2192.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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