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Mouse Anti-HNRNPK Recombinant Antibody (A254) (CBMAB-AP2972LY)

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Datasheet
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Basic Information

Host Animal
Mouse
Clone
A254
Application
IHC, WB
Immunogen
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminal of the hnRNP-K protein and mouse myeloma Ag8563 cells.
Specificity
Chicken, Human, Mouse, Rabbit, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
Affinity purity
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
heterogeneous nuclear ribonucleoprotein K
Introduction
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human3190
Mouse15387
Rat117282
Rabbit100008849
Chicken427458
UniProt ID
HumanP61978
MouseP61979
RatP61980
RabbitO19049
ChickenQ5ZIQ3
Alternative Names
Heterogeneous Nuclear Ribonucleoprotein K; Transformation Upregulated Nuclear Protein; HNRPK; TUNP; Transformation Up-Regulated Nuclear Protein; DC-Stretch Binding Protein; HnRNP K; AUKS; CSBP;
Function
X2068M
Biological Process
mRNA splicing, via spliceosome Source: UniProtKB
Negative regulation of apoptotic process Source: MGI
Negative regulation of mRNA splicing, via spliceosome Source: Ensembl
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of low-density lipoprotein receptor activity Source: BHF-UCL
Positive regulation of receptor-mediated endocytosis Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Regulation of gene expression Source: GO_Central
Regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: Ensembl
Regulation of low-density lipoprotein particle clearance Source: BHF-UCL
Regulation of mRNA splicing, via spliceosome Source: GO_Central
Regulation of transcription by RNA polymerase II Source: GO_Central
RNA processing Source: ProtInc
Signal transduction Source: ProtInc
Cellular Location
Nucleoplasm; Cytoplasm; Podosome. Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702).
Involvement in disease
Au-Kline syndrome (AUKS):
A disorder characterized by intellectual disability, facial dysmorphism, cardiac defects, and connective tissue and skeletal abnormalities. Dysmorphic features include long palpebral fissures, ptosis, a broad prominent nasal bridge, hypoplastic alae nasi, an open downturned mouth, ears with underdeveloped and thick helices, high palate, and a unique tongue with a prominent median crease. Hypotonia, hyporeflexia, and high pain tolerance are additional features.
PTM
Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions.
Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Mahale, S., Setia, M., Prajapati, B., Subhash, S., Yadav, M. P., Thankaswamy Kosalai, S., ... & Kanduri, M. (2022). HnRNPK maintains single strand RNA through controlling double-strand RNA in mammalian cells. Nature Communications, 13(1), 4865.

Braems, E., Bercier, V., Van Schoor, E., Heeren, K., Beckers, J., Fumagalli, L., ... & Van Den Bosch, L. (2022). HNRNPK alleviates RNA toxicity by counteracting DNA damage in C9orf72 ALS. Acta Neuropathologica, 144(3), 465-488.

Lee, W. J., Shin, C. H., Ji, H., Jeong, S. D., Park, M. S., Won, H. H., ... & Kim, H. H. (2021). hnRNPK-regulated LINC00263 promotes malignant phenotypes through miR-147a/CAPN2. Cell Death & Disease, 12(4), 290.

Peng, C., Tan, Y., Yang, P., Jin, K., Zhang, C., Peng, W., ... & Sun, Y. (2021). Circ-GALNT16 restrains colorectal cancer progression by enhancing the SUMOylation of hnRNPK. Journal of Experimental & Clinical Cancer Research, 40(1), 272.

Wang, Z., Qiu, H., He, J., Liu, L., Xue, W., Fox, A., ... & Xu, J. (2020). The emerging roles of hnRNPK. Journal of cellular physiology, 235(3), 1995-2008.

Peng, W. Z., Liu, J. X., Li, C. F., Ma, R., & Jie, J. Z. (2019). hnRNPK promotes gastric tumorigenesis through regulating CD44E alternative splicing. Cancer cell international, 19(1), 1-11.

Xu, Y., Wu, W., Han, Q., Wang, Y., Li, C., Zhang, P., & Xu, H. (2019). Post-translational modification control of RNA-binding protein hnRNPK function. Open biology, 9(3), 180239.

Xu, Y., Wu, W., Han, Q., Wang, Y., Li, C., Zhang, P., & Xu, H. (2019). New insights into the interplay between non-coding RNAs and RNA-binding protein HnRNPK in regulating cellular functions. Cells, 8(1), 62.

Gao, T., Liu, X., He, B., Nie, Z., Zhu, C., Zhang, P., & Wang, S. (2018). Exosomal lncRNA 91H is associated with poor development in colorectal cancer by modifying HNRNPK expression. Cancer cell international, 18, 1-10.

Liu, L., Luo, C., Luo, Y., Chen, L., Liu, Y., Wang, Y., ... & Feng, Y. (2018). MRPL33 and its splicing regulator hnRNPK are required for mitochondria function and implicated in tumor progression. Oncogene, 37(1), 86-94.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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