Mouse Anti-HPSE Recombinant Antibody (A261) (CBMAB-AP2952LY)

Name: Mouse Anti-HPSE Recombinant Antibody (A261)
SKU: CBMAB-AP2952LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A261

Application

ICC, IHC, IP, WB

Immunogen

Recombinant Heparanase (HPA)

Specificity

Human

Antibody Isotype

IgG2a, λ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

Affinity purity

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Heparanase

Introduction

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Entrez Gene ID

10855

UniProt ID

Q9Y251

Alternative Names

Heparanase; Endo-Glucoronidase; Heparanase-1; HPSE1; HPA1; HPR1;

Function

MAA711Hu21

Biological Process

Angiogenesis involved in wound healing Source: GO_Central
Cell-matrix adhesion Source: UniProtKB
Establishment of endothelial barrier Source: Ensembl
Factor XII activation Source: ComplexPortal
Heparan sulfate proteoglycan catabolic process Source: ComplexPortal
Heparin metabolic process Source: Ensembl
Inflammatory response Source: ComplexPortal
Positive regulation of angiogenesis Source: ComplexPortal
Positive regulation of autophagy Source: ComplexPortal
Positive regulation of blood coagulation Source: ComplexPortal
Positive regulation of cell-matrix adhesion Source: ComplexPortal
Positive regulation of cell migration Source: ComplexPortal
Positive regulation of cell population proliferation Source: ComplexPortal
Positive regulation of hair follicle development Source: Ensembl
Positive regulation of osteoblast proliferation Source: UniProtKB
Positive regulation of protein kinase B signaling Source: UniProtKB
Positive regulation of substrate adhesion-dependent cell spreading Source: ComplexPortal
Positive regulation of vascular endothelial growth factor production Source: UniProtKB
Protein transmembrane transport Source: Ensembl
Proteoglycan metabolic process Source: ProtInc
Regulation of hair follicle development Source: UniProtKB
Response to organic substance Source: Ensembl
Vascular wound healing Source: Ensembl

Cellular Location

Nucleus; Secreted; Lysosome membrane. Proheparanase is secreted via vesicles of the Golgi. Interacts with cell membrane heparan sulfate proteoglycans (HSPGs). Endocytosed and accumulates in endosomes. Transferred to lysosomes where it is proteolytically cleaved to produce the active enzyme. Under certain stimuli, transferred to the cell surface. Associates with lipid rafts. Colocalizes with SDC1 in endosomal/lysosomal vesicles. Accumulates in perinuclear lysosomal vesicles. Heparin retains proheparanase in the extracellular medium (By similarity).

PTM

Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.
N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.

Yang, Y., Yuan, F., Zhou, H., Quan, J., Liu, C., Wang, Y., ... & Yu, X. (2023). Potential roles of heparanase in cancer therapy: Current trends and future direction. Journal of Cellular Physiology, 238(5), 896-917.

de Boer, C., Armstrong, Z., Lit, V. A., Barash, U., Ruijgrok, G., Boyango, I., ... & Wu, L. (2022). Mechanism-based heparanase inhibitors reduce cancer metastasis in vivo. Proceedings of the National Academy of Sciences, 119(31), e2203167119.

Gallard, C., Lebsir, N., Khursheed, H., Reungoat, E., Plissonnier, M. L., Bré, J., ... & Grigorov, B. (2022). Heparanase-1 is upregulated by hepatitis C virus and favors its replication. Journal of Hepatology, 77(1), 29-41.

Yang, W. J., Shi, L., Wang, X. M., & Yang, G. W. (2021). Heparanase is a novel biomarker for immune infiltration and prognosis in breast cancer. Aging (Albany NY), 13(16), 20836.

Koganti, R., Suryawanshi, R., & Shukla, D. (2020). Heparanase, cell signaling, and viral infections. Cellular and Molecular Life Sciences, 77, 5059-5077.

Agelidis, A., & Shukla, D. (2020). Heparanase, heparan sulfate and viral infection. Heparanase: From Basic Research to Clinical Applications, 759-770.

Buijsers, B., Yanginlar, C., de Nooijer, A., Grondman, I., Maciej-Hulme, M. L., Jonkman, I., ... & van der Vlag, J. (2020). Increased plasma heparanase activity in COVID-19 patients. Frontiers in immunology, 11, 575047.

Theodoro, T. R., Matos, L. L., Cavalheiro, R. P., Justo, G. Z., Nader, H. B., & Pinhal, M. A. S. (2019). Crosstalk between tumor cells and lymphocytes modulates heparanase expression. Journal of Translational Medicine, 17(1), 1-14.

Wei, R. R., Sun, D. N., Yang, H., Yan, J., Zhang, X., Zheng, X. L., ... & Ding, J. (2018). CTC clusters induced by heparanase enhance breast cancer metastasis. Acta Pharmacologica Sinica, 39(8), 1326-1337.

Lv, Q., Wu, K., Liu, F., Wu, W., Chen, Y., & Zhang, W. (2018). Interleukin‑17A and heparanase promote angiogenesis and cell proliferation and invasion in cervical cancer. International Journal of Oncology, 53(4), 1809-1817.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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