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Mouse Anti-KHDRBS3 Antibody (1D3) (CBMAB-0580-YC)

Provided herein are mouse monoclonal antibodies against Human KHDRBS3. The antibody clone 1D3 can be used for immunoassay techniques, such as IP and MA.
See all KHDRBS3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1D3
Antibody Isotype
IgG1
Application
IP, MA

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
KHDRBS3
Introduction
KHDRBS3 (KH domain containing, RNA binding, signal transduction associated 3) is a protein that in humans is encoded by the KHDRBS3 gene. KHDRBS3 has activities of nucleic acid binding and RNA binding and play a role in signaling by PTK6 and signaling by GPCR. An important paralog of this gene is KHDRBS2. Diseases associated with KHDRBS3 include Transvestism and Fetishism.
Entrez Gene ID
UniProt ID
Alternative Names
Etle; etoile; SALP; SLM-2; SLM2; T-STAR
Function
Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species according to (PubMed:22253824).
RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). Can regulate CD44 alternative splicing in a Ras pathway-dependent manner (By similarity).
In cooperation with HNRNPA1 modulates alternative splicing of BCL2L1 by promoting splicing toward isoform Bcl-X(S), and of SMN1 (PubMed:17371836, PubMed:20186123).
Can regulate alternative splicing of NRXN1 and NRXN3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners. In a neuronal activity-dependent manner cooperates synergistically with KHDRBS2/SLIM-1 in regulation of NRXN1 exon skipping at AS4. The cooperation with KHDRBS2/SLIM-1 is antagonistic for regulation of NXRN3 alternative splicing at AS4 (By similarity).
Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.
Biological Process
G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentTAS:GO_Central
G2/M transition of mitotic cell cycleISS:UniProtKB
mRNA processingManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Negative regulation of transcription, DNA-templatedISS:UniProtKB
Positive regulation of RNA export from nucleusManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of translational initiationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of alternative mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIBA:GO_Central
Regulation of RNA export from nucleusISS:UniProtKB
SpermatogenesisIEA:Ensembl
T cell receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus; Cytoplasm; Membrane. Predominantly located in the nucleus but also located partially in the cytoplasm.
PTM
Tyrosine phosphorylated by several non-receptor tyrosine kinases including LCK, FYN and JAK3. Also tyrosine phosphorylated by the non-receptor tyrosine kinase SRMS in an EGF-dependent manner (PubMed:29496907).
Negatively correlates with ability to bind RNA but required for many interactions with proteins. Phosphorylation by PTK6 negatively regulates its RNA binding ability. Phosphorylation by PTK6 at Tyr-440 dictates the nuclear localization of KHDRBS1. Phosphorylation at Tyr-387 disrupts interaction with APC. Phosphorylation at tyrosine residues by FYN inverts activity on modulation of BCL2L1 alternative splicing
Acetylated. Positively correlates with ability to bind RNA.
Arginine methylation is required for nuclear localization. Also can affect interaction with other proteins. Inhibits interaction with Src-like SH3 domains, but not interaction with WW domains of WBP4/FBP21 AND FNBP4/FBP30.

Zhao, M., Zhang, Y., Li, L., Liu, X., Zhou, W., Wang, C., & Tang, Y. (2023). KHDRBS3 accelerates glycolysis and promotes malignancy of hepatocellular carcinoma via upregulating 14-3-3ζ. Cancer Cell International, 23(1), 244.

Feng, M., Tu, W., Zhou, Q., Du, Y., Xu, K., & Wang, Y. (2023). circHECTD1 Promotes the Proliferation and Migration of Human Brain Vascular Smooth Muscle Cells via Interacting with KHDRBS3 to Stabilize EZH2 mRNA Expression. Journal of Inflammation Research, 1311-1323.

Paradis, P., Berillo, O., Coelho, S. C., Ferreira, N. D., Richer, C., sinnett, D., & Schiffrin, E. L. (2023). Abstract P312: Three-Month Human Endothelin-1 Endothelial Overexpression Up-Regulated Khdrbs3 And Caused Vegfa And Nrp2 Alternative Splicing In Mesenteric Arteries Of Male Mice. Hypertension, 80(Suppl_1), AP312-AP312.

Wu, X., Qiu, L., Feng, H., Zhang, H., Yu, H., Du, Y., ... & Jiang, H. (2022). KHDRBS3 promotes paclitaxel resistance and induces glycolysis through modulated MIR17HG/CLDN6 signaling in epithelial ovarian cancer. Life Sciences, 293, 120328.

Lu, X., Ma, L., & Yuan, Y. (2022). Knockout KHDRBS3 inhibits cell proliferation and glucocorticoid resistance in acute lymphoblastic leukemia. Molecular & Cellular Toxicology, 1-8.

Ukai, S., Sakamoto, N., Taniyama, D., Harada, K., Honma, R., Maruyama, R., ... & Yasui, W. (2021). KHDRBS3 promotes multi‐drug resistance and anchorage‐independent growth in colorectal cancer. Cancer Science, 112(3), 1196-1208.

Ukai, S., Honma, R., Sakamoto, N., Yamamoto, Y., Pham, Q. T., Harada, K., ... & Yasui, W. (2020). Molecular biological analysis of 5-FU-resistant gastric cancer organoids; KHDRBS3 contributes to the attainment of features of cancer stem cell. Oncogene, 39(50), 7265-7278.

Wu, P., Gao, Y., Shen, S., Xue, Y., Liu, X., Ruan, X., ... & Wang, P. (2019). KHDRBS3 regulates the permeability of blood–tumor barrier via cDENND4C/miR-577 axis. Cell death & disease, 10(7), 536.

Berillo, O., Coelho, S. C., Huo, K. G., Ouerd, S., Richer, C., Sinnett, D., ... & Schiffrin, E. L. (2018). Abstract P151: Short-and Long-Term Induction of Human Endothelin-1 Overexpression in Mice Up-Regulated the Expression of Khdrbs3 in Mesenteric Arteries. Hypertension, 72(Suppl_1), AP151-AP151.

Matsumoto, Y., Itou, J., Sato, F., & Toi, M. (2018). SALL4‐KHDRBS3 network enhances stemness by modulating CD 44 splicing in basal‐like breast cancer. Cancer medicine, 7(2), 454-462.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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