Mouse Anti-KLHL12 Recombinant Antibody (13B455) (CBMAB-K1173-LY)

Name: Mouse Anti-KLHL12 Recombinant Antibody (13B455)
SKU: CBMAB-K1173-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

13B455

Application

ELISA, WB

Immunogen

Purified recombinant fragment of human KLHL12 expressed in E. coli

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Preservative

0.03% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Kelch Like Family Member 12

Introduction

This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Entrez Gene ID

59349

UniProt ID

Q53G59

Alternative Names

Kelch Like Family Member 12; CUL3-Interacting Protein 1; DKIR Homolog; C3IP1; Kelch-Like 12 (Drosophila); Kelch-Like Protein C3IP1; Kelch-Like Protein 12; HDKIR; DKIR;

Function

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport (PubMed:22358839, PubMed:27565346).
The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) (PubMed:22358839, PubMed:27565346).
The BCR(KLHL12) complex is also involved in neural crest specification: in response to cytosolic calcium increase, interacts with the heterodimer formed with PEF1 and PDCD6/ALG-2, leading to bridge together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export (PubMed:27716508).
As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3 (PubMed:16547521).
The BCR(KLHL12) complex also mediates polyubiquitination of DRD4 and PEF1, without leading to degradation of these proteins (PubMed:18303015, PubMed:20100572, PubMed:27716508).

Biological Process

COPII vesicle coatingManual Assertion Based On ExperimentIMP:UniProtKB
Endoplasmic reticulum to Golgi vesicle-mediated transportManual Assertion Based On ExperimentIDA:UniProtKB
Neural crest cell developmentManual Assertion Based On ExperimentIMP:UniProtKB
Neural crest formationManual Assertion Based On ExperimentIMP:UniProtKB
Protein monoubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Wnt signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasmic vesicle, COPII-coated vesicle

PTM

Ubiquitinated by the SCF(FBXL17) complex, leading to its degradation by the proteaseome: ubiquitination by the SCF(FBXL17) complex takes place when aberrant BTB domain dimers are formed.

Moretti, T., Kim, K., Tuladhar, A., & Kim, J. (2023). KLHL12 can form large COPII structures in the absence of CUL3 neddylation. Molecular Biology of the Cell, 34(3), br4.

Abe, T., Kanno, S. I., Niihori, T., Terao, M., Takada, S., & Aoki, Y. (2023). LZTR1 deficiency exerts high metastatic potential by enhancing sensitivity to EMT induction and controlling KLHL12-mediated collagen secretion. Cell Death & Disease, 14(8), 556.

Chen, Z., Wasney, G. A., Picaud, S., Filippakopoulos, P., Vedadi, M., D'Angiolella, V., & Bullock, A. N. (2020). Identification of a PGXPP degron motif in dishevelled and structural basis for its binding to the E3 ligase KLHL12. Open biology, 10(6), 200041.

Zhao, B., Payne, W. G., Sai, J., Lu, Z., Olejniczak, E. T., & Fesik, S. W. (2020). Structural elucidation of peptide binding to KLHL-12, a substrate specific adapter protein in a Cul3-ring E3 ligase complex. Biochemistry, 59(8), 964-969.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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