Mouse Anti-LRIT3 Recombinant Antibody (3E7) (CBMAB-L2027-YJ)

Name: Mouse Anti-LRIT3 Recombinant Antibody (3E7)
SKU: CBMAB-L2027-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3E7

Application

ELISA, IHC-P, WB

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.2

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

leucine-rich repeat, immunoglobulin-like and transmembrane domains 3

Introduction

LRIT3 has a fibronectin type III domain and a C-terminal transmembrane domain, as well as a leucine-rich repeat domain and immunoglobulin-like domain near the N-terminus. LRIT3 may regulate fibroblast growth factor receptors and affect the modification of these receptors, which are glycosylated differently in the Golgi and endoplasmic reticulum. Mutations in this gene are associated with congenital stationary night blindness, type 1F.

Entrez Gene ID

345193

UniProt ID

Q3SXY7

Alternative Names

CSNB1F; FIGLER4; leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3; fibronectin type III, immunoglobulin and leucine rich repeat domains 4; leucine-rich repeat, immunoglobulin-like and transmembrane domains 3

Function

Plays a role in the synapse formation and synaptic transmission between cone photoreceptor cells and retinal bipolar cells (By similarity).
Required for normal transmission of a light-evoked stimulus from the cone photoreceptor cells to the ON-bipolar cells and ON-ganglion cells in the inner retina (PubMed:28334377).
Required in retinal ON-bipolar cells for normal localization of the cation channel TRPM1 at dendrite tips (By similarity).
Seems to play a specific role in synaptic contacts made by ON-bipolar cells with cone photoreceptor pedicles (By similarity).
May also have a role in cone synapse formation (By similarity).
Might facilitate FGFR1 exit from the endoplasmic reticulum to the Golgi (PubMed:22673519).
Could be a regulator of the FGFRs (PubMed:22673519).

Biological Process

Regulation of fibroblast growth factor receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Response to stimulusIEA:UniProtKB-KW
Visual perceptionIEA:UniProtKB-KW

Cellular Location

Cell projection, dendrite
Perikaryon
Endoplasmic reticulum membrane
Punctate expression at dendrite tips.

Involvement in disease

Night blindness, congenital stationary, 1F (CSNB1F):
An autosomal recessive form of congenital stationary night blindness, a non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia.

Topology

Lumenal: 20-582
Helical: 583-603
Cytoplasmic: 604-679

PTM

Glycosylated.

Takahashi, K., Kwok, J. C., Sato, Y., Aguirre, G. D., & Miyadera, K. (2023). Long-term functional rescue following a AAV-based gene therapy in canine model of LRIT3-congenital stationary night blindness. Investigative Ophthalmology & Visual Science, 64(8), 752-752.

Gregg, R. G., Hasan, N., & Borghuis, B. G. (2023). LRIT3 expression in cone photoreceptors restores post-synaptic bipolar cell signalplex assembly and partial function in Lrit3−/− mice. Iscience, 26(4).

Takahashi, K., Kwok, J. C., Sato, Y., Aguirre, G. D., & Miyadera, K. (2023). Extended functional rescue following AAV gene therapy in a canine model of LRIT3-congenital stationary night blindness. Vision Research, 209, 108260.

Miyadera, K., Santana, E., Roszak, K., Iffrig, S., Visel, M., Iwabe, S., ... & Aguirre, G. D. (2022). Targeting ON-bipolar cells by AAV gene therapy stably reverses LRIT3-congenital stationary night blindness. Proceedings of the National Academy of Sciences, 119(13), e2117038119.

Gregg, R. G., Hasan, N., & Borghuis, B. G. (2022). LRIT3 expression in cone photoreceptors restores post-synaptic bipolar cell signalplex assembly and function in Lrit3-/-mice. bioRxiv, 2022-08.

Miyadera, K., Roszak, K., Garcia, A. R., Santana, E., Iffrig, S. M., Visel, M., ... & Aguirre, G. D. (2020). AAV gene therapy restores ON-bipolar cell function in a canine model of LRIT3-congenital stationary night blindness. Investigative Ophthalmology & Visual Science, 61(7), 2298-2298.

Hasan, N., Pangeni, G., Ray, T. A., Fransen, K. M., Noel, J., Borghuis, B. G., ... & Gregg, R. G. (2020). LRIT3 is required for nyctalopin expression and normal ON and OFF pathway signaling in the retina. eneuro, 7(1).

Das, R. G., Becker, D., Jagannathan, V., Goldstein, O., Santana, E., Carlin, K., ... & Miyadera, K. (2019). Genome-wide association study and whole-genome sequencing identify a deletion in LRIT3 associated with canine congenital stationary night blindness. Scientific reports, 9(1), 14166.

Hasan, N., Pangeni, G., Cobb, C. A., Ray, T. A., Nettesheim, E. R., Ertel, K. J., ... & Gregg, R. G. (2019). Presynaptic expression of LRIT3 transsynaptically organizes the postsynaptic glutamate signaling complex containing TRPM1. Cell reports, 27(11), 3107-3116.

Hasan, N., Cobb, C., Nettesheim, E. R., Lipinski, D. M., McCall, M. A., & Gregg, R. G. (2019). Role of presynaptic LRIT3 expression in the restoration of dim light vision. Investigative Ophthalmology & Visual Science, 60(9), 4788-4788.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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