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Mouse Anti-LRP6 Recombinant Antibody (CBYCL-471) (CBMAB-L0372-YC)

Provided herein is a Mouse monoclonal antibody against Human LRP6. The antibody can be used for immunoassay techniques, such as WB, ICC, IHC-P, IHC-Fr, ELISA.
See all LRP6 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYCL-471
Antibody Isotype
IgG
Application
WB, ICC, IHC-P, IHC-Fr, ELISA

Basic Information

Immunogen
Low Density Lipoprotein Receptor Related Protein 6
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH7.4, with 0.02% sodium azide, 50% glycerol
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
LRP6
Introduction
LRP6 is a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. LRP6 functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. LRP6 undergoes gamma-secretase dependent RIP-processing but the precise locations of the cleavage sites have not been determined.
Entrez Gene ID
4040
UniProt ID
O75581
Alternative Names
ADCAD2; STHAG7
Function
Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity).
Biological Process
Canonical Wnt signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Canonical Wnt signaling pathway involved in neural crest cell differentiation1 PublicationIC:BHF-UCL
Canonical Wnt signaling pathway involved in regulation of cell proliferation1 PublicationIC:BHF-UCL
Cell-cell adhesionIEA:Ensembl
Cellular response to cholesterolManual Assertion Based On ExperimentIMP:BHF-UCL
Chemical synaptic transmissionManual Assertion Based On ExperimentIBA:GO_CentralDopaminergic neuron differentiationISS:ParkinsonsUK-UCL
Midbrain dopaminergic neuron differentiationManual Assertion Based On ExperimentTAS:ParkinsonsUK-UCL
Negative regulation of protein kinase activityManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of protein phosphorylationManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of protein serine/threonine kinase activityManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of smooth muscle cell apoptotic processManual Assertion Based On ExperimentIMP:BHF-UCL
Neural crest cell differentiationManual Assertion Based On ExperimentIDA:BHF-UCL
Neural crest formationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of cell cycleManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of cytosolic calcium ion concentrationIEA:Ensembl
Positive regulation of DNA-binding transcription factor activityManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:BHF-UCL
Protein localization to plasma membraneManual Assertion Based On ExperimentIPI:ParkinsonsUK-UCL
Receptor-mediated endocytosis involved in cholesterol transportManual Assertion Based On ExperimentIBA:GO_Central
Response to peptide hormoneIEA:Ensembl
Wnt signaling pathwayManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Wnt signaling pathway involved in midbrain dopaminergic neuron differentiationManual Assertion Based On ExperimentTAS:ParkinsonsUK-UCL
Wnt signaling pathway involved in somitogenesisManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cell membrane
Endoplasmic reticulum
Membrane raft
On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma membrane by MESD (By similarity).
Involvement in disease
Coronary artery disease, autosomal dominant, 2 (ADCAD2):
A common heart disease characterized by reduced or absent blood flow in one or more of the arteries that encircle and supply the heart. Its most important complication is acute myocardial infarction.
Tooth agenesis, selective, 7 (STHAG7):
An autosomal dominant form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth).
Topology
Extracellular: 20-1370
Helical: 1371-1393
Cytoplasmic: 1394-1613
PTM
Dual phosphorylation of cytoplasmic PPPSP motifs sequentially by GSK3 and CK1 is required for AXIN1-binding, and subsequent stabilization and activation of beta-catenin via preventing GSK3-mediated phosphorylation of beta-catenin. Phosphorylated, in vitro, by GRK5/6 within and outside the PPPSP motifs. Phosphorylation at Ser-1490 by CDK14 during G2/M phase leads to regulation of the Wnt signaling pathway during the cell cycle. Phosphorylation by GSK3B is induced by RPSO1 binding and inhibited by DKK1. Phosphorylated, in vitro, by casein kinase I on Thr-1479.
Undergoes gamma-secretase-dependent regulated intramembrane proteolysis (RIP). The extracellular domain is first released by shedding, and then, through the action of gamma-secretase, the intracellular domain (ICD) is released into the cytoplasm where it is free to bind to GSK3B and to activate canonical Wnt signaling.
Palmitoylation on the two sites near the transmembrane domain leads to release of LRP6 from the endoplasmic reticulum.
Mono-ubiquitinated which retains LRP6 in the endoplasmic reticulum. Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
N-glycosylation is required for cell surface location.

Zhang, X., Yang, G., Liu, W., Liu, Q., Wang, Z., Fan, K., ... & Huang, Y. (2023). Screening and Identification of ssDNA Aptamers for Low-Density Lipoprotein (LDL) Receptor-Related Protein 6. Molecules, 28(9), 3838.

Enayatkhani, M., Salimi, M., Azadmanesh, K., & Teimoori-Toolabi, L. (2022). In-silico identification of new inhibitors for Low-density lipoprotein receptor-related protein6 (LRP6). Journal of Biomolecular Structure and Dynamics, 40(10), 4440-4450.

Liu, Z., Li, C., Liu, M., Song, Z., Moyer, M. P., & Su, D. (2022). The low-density lipoprotein receptor-related protein 6 pathway in the treatment of intestinal barrier dysfunction induced by hypoxia and intestinal microbiota through the Wnt/β-catenin pathway. International Journal of Biological Sciences, 18(11), 4469.

Jeong, W., & Jho, E. H. (2021). Regulation of the low-density lipoprotein receptor-related protein LRP6 and its association with disease: Wnt/β-catenin signaling and beyond. Frontiers in cell and developmental biology, 9, 714330.

Wang, Y., Chen, Z., Li, Y., Ma, L., Zou, Y., Wang, X., ... & Gong, H. (2020). Low density lipoprotein receptor related protein 6 (LRP6) protects heart against oxidative stress by the crosstalk of HSF1 and GSK3β. Redox Biology, 37, 101699.

Kang, S. (2020). Low-density lipoprotein receptor-related protein 6-mediated signaling pathways and associated cardiovascular diseases: diagnostic and therapeutic opportunities. Human Genetics, 139, 447-459.

Jeong, W., Kim, S., Lee, U., Zhong, Z. A., Savitsky, M., Kwon, H., ... & Jho, E. H. (2020). LDL receptor‐related protein LRP 6 senses nutrient levels and regulates Hippo signaling. EMBO reports, 21(9), e50103.

Brance, M. L., Brun, L. R., Cóccaro, N. M., Aravena, A., Duan, S., Mumm, S., & Whyte, M. P. (2020). High bone mass from mutation of low-density lipoprotein receptor-related protein 6 (LRP6). Bone, 141, 115550.

Roslan, Z., Muhamad, M., Selvaratnam, L., & Ab-Rahim, S. (2019). The roles of low-density lipoprotein receptor-related proteins 5, 6, and 8 in cancer: a review. Journal of oncology, 2019.

Alhusseiny, A. H., Khaleel, Z. J., & Al-Nimer, M. S. (2019). Serum low density lipoprotein receptor related protein-6 is a discriminator of occluded‎ coronary artery assessed by coronary angiogram. Journal of Ideas in Health, 2(2), 95-101.

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For research use only. Not intended for any clinical use.

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