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Mouse Anti-MAP2K1 Recombinant Antibody (1B5) (CBMAB-A5189-LY)

The product is antibody recognizes MAP2K1. The antibody 1B5 immunoassay techniques such as: WB, ELISA.
See all MAP2K1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1B5
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
MAP2K1 (NP_002746, 294 a.a. ~ 393 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
mitogen-activated protein kinase kinase 1
Introduction
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq]
Entrez Gene ID
5604
UniProt ID
Q02750
Alternative Names
MAPKK1; MEK1; MKK1; PRKMK1
Function
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126).
Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Biological Process
Bergmann glial cell differentiationIEA:Ensembl
Cell motilityIEA:Ensembl
Cellular senescenceManual Assertion Based On ExperimentIMP:BHF-UCL
Cerebellar cortex formationIEA:Ensembl
ChemotaxisManual Assertion Based On ExperimentTAS:ProtInc
Epithelial cell proliferation involved in lung morphogenesisIEA:Ensembl
ERK1 and ERK2 cascadeTAS:Reactome
Face developmentIEA:Ensembl
Heart developmentIEA:Ensembl
Keratinocyte differentiationIEA:Ensembl
Labyrinthine layer developmentIEA:Ensembl
MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of cell population proliferationManual Assertion Based On ExperimentIDA:BHF-UCL
Neuron differentiationManual Assertion Based On ExperimentIBA:GO_Central
Placenta blood vessel developmentIEA:Ensembl
Positive regulation of axonogenesisIEA:Ensembl
Positive regulation of ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of gene expressionManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of production of miRNAs involved in gene silencing by miRNAManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of protein serine/threonine kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:BHF-UCL
Protein phosphorylationManual Assertion Based On ExperimentIDA:FlyBase
Regulation of axon regenerationIEA:Ensembl
Regulation of early endosome to late endosome transportManual Assertion Based On ExperimentTAS:UniProtKB
Regulation of Golgi inheritanceManual Assertion Based On ExperimentTAS:UniProtKB
Regulation of stress-activated MAPK cascadeManual Assertion Based On ExperimentTAS:UniProtKB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Thymus developmentIEA:Ensembl
Thyroid gland developmentIEA:Ensembl
Trachea formationIEA:Ensembl
Cellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body
Cytoplasm
Nucleus
Membrane
Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis (PubMed:14737111).
Membrane localization is probably regulated by its interaction with KSR1 (PubMed:10409742).
Involvement in disease
Cardiofaciocutaneous syndrome 3 (CFC3):
A form of cardiofaciocutaneous syndrome, a multiple congenital anomaly disorder characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. Distinctive features of CFC3 include macrostomia and horizontal shape of palpebral fissures.
Melorheostosis, isolated (MEL):
A sclerosing bone disorder characterized by hyperostosis of the cortex of tubular bones, frequently involving one limb. The lesions may be accompanied by abnormalities of adjacent soft tissue, joint contractures, sclerodermatous skin lesions, muscle atrophy, or hemangioma.
PTM
Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity (PubMed:29433126, PubMed:8131746).
Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK (PubMed:16129686).
MAPK1/ERK2 phosphorylation of Thr-292 oCcurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK (PubMed:16129686).
Autophosphorylated at Ser-218 and Ser-222, autophosphosphorylation is promoted by NEK10 following UV irradiation (PubMed:20956560).
(Microbial infection) Acetylation by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.

Alomari, A. K., Harms, P. W., Andea, A. A., & Warren, S. J. (2023). MAP2K1‐mutated melanocytic tumors have reproducible histopathologic features and share similarities with melanocytic tumors with BRAF V600E mutations. Journal of Cutaneous Pathology, 50(12), 1083-1093.

Sudduth, C. L., Smits, P. J., Vivero, M. P., Cheng, Y. S., Ad, M., Konczyk, D. J., ... & Greene, A. K. (2023). Arteriovenous malformation Map2k1 mutation affects vasculogenesis. Scientific Reports, 13(1), 11074.

Mizuno, S., Ikegami, M., Koyama, T., Sunami, K., Ogata, D., Kage, H., ... & Kohsaka, S. (2023). High-throughput functional evaluation of MAP2K1 variants in cancer. Molecular Cancer Therapeutics, 22(2), 227-239.

Chuang, J., Wang, C., Guo, Y., Valenzuela, V., Wu, J., & Fakih, M. (2021). MAP2K1 mutations in advanced colorectal cancer predict poor response to anti-EGFR therapy and to vertical targeting of MAPK pathway. Clinical Colorectal Cancer, 20(1), 72-78.

Donati, M., Nosek, D., Waldenbäck, P., Martinek, P., Jonsson, B. A., Galgonkova, P., ... & Kazakov, D. V. (2021). MAP2K1-mutated melanocytic neoplasms with a SPARK-like morphology. The American Journal of Dermatopathology, 43(6), 412-417.

Chen, J., Che, L., Xu, C., Zhao, S., Yang, J., Li, M., ... & Shen, Y. (2020). Cardio-facio-cutaneous syndrome-associated pathogenic MAP2K1 variants activate autophagy. Gene, 733, 144369.

Smits, P. J., Konczyk, D. J., Sudduth, C. L., Goss, J. A., & Greene, A. K. (2020). Endothelial MAP2K1 mutations in arteriovenous malformation activate the RAS/MAPK pathway. Biochemical and biophysical research communications, 529(2), 450-454.

Sunshine, J. C., Kim, D., Zhang, B., Compres, E. V., Khan, A. U., Busam, K. J., & Gerami, P. (2020). Melanocytic neoplasms with MAP2K1 in frame deletions and spitz morphology. The American Journal of Dermatopathology, 42(12), 923.

Jha, S., Fratzl‐Zelman, N., Roschger, P., Papadakis, G. Z., Cowen, E. W., Kang, H., ... & Bhattacharyya, T. (2019). Distinct clinical and pathological features of melorheostosis associated with somatic MAP2K1 mutations. Journal of Bone and Mineral Research, 34(1), 145-156.

Jain, A. P., Patel, K., Pinto, S., Radhakrishnan, A., Nanjappa, V., Kumar, M., ... & Gowda, H. (2019). MAP2K1 is a potential therapeutic target in erlotinib resistant head and neck squamous cell carcinoma. Scientific reports, 9(1), 18793.

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For research use only. Not intended for any clinical use.

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