Mouse Anti-MAPK8 Recombinant Antibody (A727) (CBMAB-AP10966LY)

Name: Mouse Anti-MAPK8 Recombinant Antibody (A727)
SKU: CBMAB-AP10966LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A727

Application

IF, WB

Immunogen

Purified recombinant human JNK1 protein fragments expressed in Ecoli

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

Affinity purity

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Mitogen-Activated Protein Kinase 8

Entrez Gene ID

Human	5599
Mouse	26419
Rat	116554

UniProt ID

Human	P45983
Mouse	Q91Y86
Rat	P49185

Alternative Names

JNK 46, JNK 55, JNK, JNK1, JNK1A2, JNK2, JNK21B1/2, JNK2ALPHA, JNK2B, JNK2BETA, JNK3, JNK3A, MAP kinase 8, MAP kinase 9, MAP kinase p49 3F12, MAPK 8, MAPK 9, MAPK10, MAPK8, MAPK9, MGC50974, Mitogen activated protein kinase 10, Mitogen activated protein kinase 8, Mitogen activated protein kinase 9,

Function

MB0184

Biological Process

Cellular response to amino acid starvationManual Assertion Based On ExperimentIDA:CAFA
Cellular response to cadmium ionManual Assertion Based On ExperimentIMP:CAFA
Cellular response to lipopolysaccharideManual Assertion Based On ExperimentIDA:MGI
Cellular response to mechanical stimulusManual Assertion Based On ExperimentIEP:UniProtKB
Cellular response to reactive oxygen speciesManual Assertion Based On ExperimentIMP:CAFA
Cellular senescenceTAS:Reactome
Fc-epsilon receptor signaling pathwayTAS:Reactome
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
JNK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
JUN phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of protein bindingManual Assertion Based On ExperimentIDA:UniProtKB
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Peptidyl-threonine phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of apoptotic processManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cell killingTAS:Reactome
Positive regulation of cyclase activityManual Assertion Based On ExperimentIMP:CACAO
Positive regulation of deacetylase activityManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of gene expressionManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathwayTAS:Reactome
Positive regulation of protein metabolic processManual Assertion Based On ExperimentIMP:CACAO
Protein phosphorylationManual Assertion Based On ExperimentIDA:CAFA
Regulation of circadian rhythmISS:UniProtKB
Regulation of DNA replication origin bindingManual Assertion Based On ExperimentIMP:CAFA
Regulation of DNA-binding transcription factor activityTAS:Reactome
Regulation of macroautophagyManual Assertion Based On ExperimentTAS:ParkinsonsUK-UCL
Regulation of protein localizationManual Assertion Based On ExperimentIDA:BHF-UCL
Response to mechanical stimulusManual Assertion Based On ExperimentIBA:GO_Central
Response to oxidative stressManual Assertion Based On ExperimentIDA:UniProtKB
Response to UVManual Assertion Based On ExperimentIDA:MGI
Rhythmic processIEA:UniProtKB-KW
Stress-activated MAPK cascadeManual Assertion Based On ExperimentIDA:CAFA

Cellular Location

Cytoplasm
Nucleus
Cell junction, synapse
In the cortical neurons, predominantly cytoplasmic and associated with the Golgi apparatus and endosomal fraction. Increased neuronal activity increases phosphorylated form at synapses (By similarity).
Colocalizes with POU5F1 in the nucleus.

PTM

Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme (PubMed:11062067).
Phosphorylated by TAOK2 (PubMed:17158878).
May be phosphorylated at Thr-183 and Tyr-185 by MAP3K1/MEKK1 (PubMed:17761173).
Phosphorylated form is more concentrated at synapses than none-phosphorylated (By similarity).

Chai, F., Peng, H., Qin, L., Liu, C., Zeng, Y., Wang, R., ... & Wang, C. (2024). MicroRNA miR-181d-5p regulates the MAPK signaling pathway by targeting mitogen-activated protein kinase 8 (MAPK8) to improve lupus nephritis. Gene, 893, 147961.

Ma, M., Luo, Q., Fan, L., Li, W., Li, Q., Meng, Y., ... & Hocher, B. (2022). The urinary exosomes derived from premature infants attenuate cisplatin-induced acute kidney injury in mice via microRNA-30a-5p/mitogen-activated protein kinase 8 (MAPK8). Bioengineered, 13(1), 1650-1665.

Xie, Q., Liu, R., Zou, Z., Feng, Y., Huang, Y., Xu, G., ... & Zhong, W. (2022). MYPT1 inhibits the metastasis of renal clear cell carcinoma via the MAPK8/N‐cadherin pathway. FEBS Open bio, 12(11), 2083-2095.

Gao, X., Sun, X., Yao, X., Wang, Y., Li, Y., Jiang, X., ... & Xu, Y. (2022). Downregulation of the Long Noncoding RNA IALNCR Targeting MAPK8/JNK1 Promotes Apoptosis and Antagonizes Bovine Viral Diarrhea Virus Replication in Host Cells. Journal of Virology, 96(17), e01113-22.

Liu, X., Li, L., Bai, J., Li, L., Fan, J., Fu, Z., & Liu, J. (2022). Long noncoding RNA plasmacytoma variant translocation 1 promotes progression of colorectal cancer by sponging microRNA‐152‐3p and regulating E2F3/MAPK8 signaling. Cancer Science, 113(1), 109-119.

Chen, W., Zheng, G., Huang, J., Zhu, L., Li, W., Guo, T., ... & Pan, X. (2021). CircMED13L_012 promotes lung adenocarcinoma progression by upregulation of MAPK8 mediated by miR-433-3p. Cancer Cell International, 21, 1-12.

Ying, N. A. N., Yifan, X. I. E., Heng, Y. A. N. G., & Zongsheng, Z. H. A. O. (2021). MicroRNA-200c Mediates the Mechanism of MAPK8 Gene Regulating Follicular Development in Sheep. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(3).

Malik, A., Pal, R., & Gupta, S. K. (2020). EGF-mediated reduced miR-92a-1-5p controls HTR-8/SVneo cell invasion through activation of MAPK8 and FAS which in turn increase MMP-2/-9 expression. Scientific Reports, 10(1), 12274.

Gong, L., Tang, H., Luo, Z., Sun, X., Tan, X., Xie, L., ... & Han, S. (2020). Tamoxifen induces fatty liver disease in breast cancer through the MAPK8/FoxO pathway. Clinical and Translational Medicine, 10(1), 137-150.

Hua, X., Chen, J., & Wu, L. (2019). Identification of candidate biomarkers associated with apoptosis in melanosis coli: GNG5, LPAR3, MAPK8, and PSMC6. Bioscience reports, 39(1), BSR20181369.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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