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Mouse Anti-MAPKAPK2 Recombinant Antibody (3B8) (CBMAB-A5282-LY)

The product is antibody recognizes MAPKAPK2. The antibody 3B8 immunoassay techniques such as: WB, ELISA.
See all MAPKAPK2 antibodies
Published Data

Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
3B8
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
MAPKAPK2 (NP_116584, 266 a.a. ~ 352 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Mitogen-Activated Protein Kinase-Activated Protein Kinase 2
Introduction
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
Entrez Gene ID
Human9261
Rat289014
UniProt ID
HumanP49137
RatF1M9C0
Alternative Names
MK2
Function
Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (PubMed:16278218).
Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (PubMed:26616734).
Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
Biological Process
3'-UTR-mediated mRNA stabilizationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIMP:UniProtKB
Cellular response to vascular endothelial growth factor stimulusManual Assertion Based On ExperimentIMP:BHF-UCL
Inflammatory responseISS:UniProtKB
Inner ear developmentIEA:Ensembl
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Leukotriene metabolic processTAS:Reactome
MacropinocytosisISS:UniProtKB
MAPK cascadeManual Assertion Based On ExperimentTAS:ProtInc
p38MAPK cascadeIEA:Ensembl
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of tumor necrosis factor productionIEA:Ensembl
Protein autophosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Protein phosphorylationManual Assertion Based On ExperimentTAS:ProtInc
Regulation of cellular response to heatTAS:Reactome
Regulation of interleukin-6 productionISS:UniProtKB
Regulation of mRNA stabilityTAS:Reactome
Regulation of tumor necrosis factor productionManual Assertion Based On ExperimentIDA:UniProtKB
Response to cytokineManual Assertion Based On ExperimentIDA:UniProtKB
Response to lipopolysaccharideISS:UniProtKB
Toll-like receptor signaling pathwayISS:UniProtKB
Vascular endothelial growth factor receptor signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular Location
Cytoplasm
Nucleus
Phosphorylation and subsequent activation releases the autoinhibitory helix, resulting in the export from the nucleus into the cytoplasm.
PTM
Sumoylation inhibits the protein kinase activity.
Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-222, Ser-272 and Thr-334.

Zhong, Z., Li, J., Zhong, J., Huang, Y., Hu, J., Zhang, P., ... & Ling, F. (2023). MAPKAPK2, a potential dynamic network biomarker of α-synuclein prior to its aggregation in PD patients. npj Parkinson's Disease, 9(1), 41.

Guffens, L., Derua, R., & Janssens, V. (2023). PME-1 sensitizes glioblastoma cells to oxidative stress-induced cell death by attenuating PP2A-B55α-mediated inactivation of MAPKAPK2-RIPK1 signaling. Cell Death Discovery, 9(1), 265.

Malona, J. O. H. N., Chuaqui, C., Seletsky, B. M., Beebe, L., Cantin, S., Van Kalken, D., ... & Corin, A. F. (2022). Discovery of CC-99677, a selective targeted covalent MAPKAPK2 (MK2) inhibitor for autoimmune disorders. Translational Research, 249, 49-73.

Gu, Y., Liu, M., Wang, Y., Huo, Y., Liu, Z., Jin, W., & Wang, G. (2022). Identification and Functional Analysis of MAPKAPK2 in Hyriopsis cumingii. Genes, 13(11), 2060.

Zhao, Y., Li, W., Zhang, K., Xu, M., Zou, Y., Qiu, X., ... & Gao, B. (2022). Revealing oxidative stress-related genes in osteoporosis and advanced structural biological study for novel natural material discovery regarding MAPKAPK2. Frontiers in Endocrinology, 13, 1052721.

Tripathi, D., Biswas, B., Manhas, A., Singh, A., Goyal, D., Gaestel, M., & Jagavelu, K. (2019). Proinflammatory effect of endothelial microparticles is mitochondria mediated and modulated through MAPKAPK2 (MAPK-activated protein kinase 2) leading to attenuation of cardiac hypertrophy. Arteriosclerosis, Thrombosis, and Vascular Biology, 39(6), 1100-1112.

Berggren, K. L., Restrepo Cruz, S., Hixon, M. D., Cowan, A. T., Keysar, S. B., Craig, S., ... & Gan, G. N. (2019). MAPKAPK2 (MK2) inhibition mediates radiation-induced inflammatory cytokine production and tumor growth in head and neck squamous cell carcinoma. Oncogene, 38(48), 7329-7341.

Wang, R., Liu, H., Shao, Y., Wang, K., Yin, S., Qiu, Y., ... & Yu, H. (2019). Sophoridine inhibits human colorectal cancer progression via targeting MAPKAPK2. Molecular Cancer Research, 17(12), 2469-2479.

Soni, S., Saroch, M. K., Chander, B., Tirpude, N. V., & Padwad, Y. S. (2019). MAPKAPK2 plays a crucial role in the progression of head and neck squamous cell carcinoma by regulating transcript stability. Journal of Experimental & Clinical Cancer Research, 38(1), 1-13.

Soni, S., Anand, P., & Padwad, Y. S. (2019). MAPKAPK2: the master regulator of RNA-binding proteins modulates transcript stability and tumor progression. Journal of Experimental & Clinical Cancer Research, 38, 1-18.

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For research use only. Not intended for any clinical use.

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