Mouse Anti-MBTD1 (AA 130-566) Recombinant Antibody (CBFYM-0786) (CBMAB-M0930-FY)

Name: Mouse Anti-MBTD1 (AA 130-566) Recombinant Antibody (CBFYM-0786)
SKU: CBMAB-M0930-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0786

Application

IP, WB

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 130-566

More Infomation

Target

Full Name

MBT DOMAIN CONTAINING 1

Introduction

MBTD1 is a Protein Coding gene. Diseases associated with MBTD1 include Endometrial Stromal Sarcoma and Endometrial Stromal Nodule. Among its related pathways are Mesodermal Commitment Pathway. An important paralog of this gene is L3MBTL2.

Entrez Gene ID

54799

UniProt ID

Q05BQ5

Alternative Names

Mbt Domain Containing 1; MBT Domain-Containing Protein 1; SA49P01

Function

Chromatin reader component of the NuA4 histone acetyltransferase complex, a multiprotein complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (PubMed:27153538, PubMed:32209463).

The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) by promoting homologous recombination (HR) (PubMed:27153538).

MBTD1 specifically recognizes and binds monomethylated and dimethylated 'Lys-20' on histone H4 (H4K20me1 and H4K20me2, respectively) (PubMed:19841675, PubMed:27153538, PubMed:32209463).

In the NuA4 complex, MBTD1 promotes recruitment of the complex to H4K20me marks by competing with TP53BP1 for binding to H4K20me (PubMed:27153538).

Following recruitment to H4K20me at DNA breaks, the NuA4 complex catalyzes acetylation of 'Lys-15' on histone H2A (H2AK15), blocking the ubiquitination mark required for TP53BP1 localization at DNA breaks, thereby promoting homologous recombination (HR) (PubMed:27153538).

Biological Process

Chromatin organization Source: UniProtKB-KW
Double-strand break repair via homologous recombination Source: UniProtKB
Embryonic skeletal system development Source: Ensembl
Histone acetylation Source: ComplexPortal
Histone H2A acetylation Source: ComplexPortal
Histone H4 acetylation Source: ComplexPortal
Negative regulation of transcription, DNA-templated Source: GO_Central
Positive regulation of double-strand break repair via homologous recombination Source: ComplexPortal
Positive regulation of transcription, DNA-templated Source: ComplexPortal
Regulation of apoptotic process Source: ComplexPortal
Regulation of cell cycle Source: ComplexPortal
Regulation of double-strand break repair Source: ComplexPortal

Cellular Location

Nucleus
Other locations
Chromosome

Involvement in disease

A chromosomal aberration involving MBTD1 is a cause of acute poorly differentiated myeloid leukemia. Translocation (10;17)(p15;q21) with ZMYND11.

Takubo, K., Htun, P. W., Ueda, T., Sera, Y., Iwasaki, M., Koizumi, M., ... & Honda, H. (2023). MBTD1 preserves adult hematopoietic stem cell pool size and function. Proceedings of the National Academy of Sciences, 120(32), e2206860120.

Devoucoux, M., Fort, V., Khelifi, G., Xu, J., Alerasool, N., Galloy, M., ... & Côté, J. (2022). Oncogenic ZMYND11-MBTD1 fusion protein anchors the NuA4/TIP60 histone acetyltransferase complex to the coding region of active genes. Cell Reports, 39(11).

Li, J., Galbo Jr, P. M., Gong, W., Storey, A. J., Tsai, Y. H., Yu, X., ... & Wang, G. G. (2021). ZMYND11-MBTD1 induces leukemogenesis through hijacking NuA4/TIP60 acetyltransferase complex and a PWWP-mediated chromatin association mechanism. Nature communications, 12(1), 1045.

Han, L., Liu, Y. J., Ricciotti, R. W., & Mantilla, J. G. (2020). A novel MBTD1‐PHF1 gene fusion in endometrial stromal sarcoma: a case report and literature review. Genes, Chromosomes and Cancer, 59(7), 428-432.

Zhang, H., Devoucoux, M., Song, X., Li, L., Ayaz, G., Cheng, H., ... & Min, J. (2020). Structural basis for EPC1-mediated recruitment of MBTD1 into the NuA4/TIP60 acetyltransferase complex. Cell reports, 30(12), 3996-4002.

Fu, D., Lu, C., Qu, X., Li, P., Chen, K., Shan, L., & Zhu, X. (2019). LncRNA TTN-AS1 regulates osteosarcoma cell apoptosis and drug resistance via the miR-134-5p/MBTD1 axis. Aging (Albany NY), 11(19), 8374.

Plesa, A., & Sujobert, P. (2019). Cannibalistic acute myeloid leukemia with ZMYND11-MBTD1 fusion. blood, 133(16), 1789.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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