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Mouse Anti-MCM3AP Recombinant Antibody (1H3) (CBMAB-G6374-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1H3
Antibody Isotype
IgG2a, κ
Application
ELISA, ICC, IF

Basic Information

Immunogen
MCM3AP (AAH13285, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.2-1 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Introduction
The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability. [provided by RefSeq, Jan 2014]
Entrez Gene ID
8888
UniProt ID
O60318
Alternative Names
Minichromosome Maintenance Complex Component 3 Associated Protein; Germinal-Centre Associated Nuclear Protein; 80 KDa MCM3-Associated Protein; MCM3 Acetylating Protein; MCM3 Acetyltransferase; MAP80; GANP; MCM3 Minichromosome Maintenance Deficient 3 (S. Cerevisiae) Associated Protein; Minichromosome Maintenance Deficient (S. Cerevisiae) 3-Associated Protein;
Function
Isoform GANP:
As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores (PubMed:20005110, PubMed:20384790, PubMed:23591820, PubMed:22307388).

Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations (PubMed:23652018).

Isoform MCM3AP:
Binds to and acetylates the replication protein MCM3. Plays a role in the initiation of DNA replication and participates in controls that ensure that DNA replication initiates only once per cell cycle (PubMed:11258703, PubMed:12226073).

Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations (PubMed:23652018).
Biological Process
mRNA export from nucleus Source: GO_Central
Nucleosome organization Source: UniProtKB
Poly(A)+ mRNA export from nucleus Source: UniProtKB
Protein transport Source: UniProtKB-KW
Somatic hypermutation of immunoglobulin genes Source: UniProtKB
Cellular Location
Isoform GANP:
Nucleus
Nucleus envelope
nuclear pore complex
nucleoplasm
Other locations
Chromosome
Note: Predominantly located at the nuclear envelope, facing the nucleus interior (PubMed:20005110, PubMed:21195085, PubMed:23591820). Localization at the nuclear pore complex requires NUP153, TPR and ALYREF/ALY (PubMed:23591820, PubMed:22307388). Also found associated with chromatin (PubMed:23652018). In B-cells, targeted to the immunoglobulin variable region genes (PubMed:23652018).
Isoform MCM3AP:
Nucleus
Cytoplasm
Note: Translocates into the nucleus in the presence of MCM3 (PubMed:12226073). Associates with chromatin possibly through interaction with MCM3 (PubMed:12226073).
Involvement in disease
Peripheral neuropathy, autosomal recessive, with or without impaired intellectual development (PNRIID):
An autosomal recessive disorder characterized by early childhood-onset of peripheral sensorimotor neuropathy, progressive distal muscle weakness, atrophy in hands and feet, and gait difficulties, often with loss of ambulation. Most affected individuals also have impaired intellectual development, although some have normal cognition. Additional features may include eye movement abnormalities, claw hands, foot deformities, and scoliosis.
More Infomation

Ghafouri-Fard, S., Khoshbakht, T., Hussen, B. M., Taheri, M., & Samadian, M. (2022). A review on the role of MCM3AP-AS1 in the carcinogenesis and tumor progression. Cancer Cell International, 22(1), 1-14.

Song, Q., Gao, J., Yang, J., Gopinath, S. C., & Shen, L. (2022). Prognostic Analysis of LncRNA MCM3AP-AS1 in Colorectal Cancer and the Mechanism of Its Effect on Tumor Cell Activity. BioMed Research International, 2022.

Yu, X., Zheng, Q., Zhang, Q., Zhang, S., He, Y., & Guo, W. (2021). MCM3AP-AS1: An indispensable cancer-related LncRNA. Frontiers in Cell and Developmental Biology, 9, 752718.

Li, X., Yu, M., & Yang, C. (2020). YY1‐mediated overexpression of long noncoding RNA MCM3AP‐AS1 accelerates angiogenesis and progression in lung cancer by targeting miR‐340‐5p/KPNA4 axis. Journal of cellular biochemistry, 121(3), 2258-2267.

Chen, Q., Xu, H., Zhu, J., Feng, K., & Hu, C. (2020). LncRNA MCM3AP-AS1 promotes breast cancer progression via modulating miR-28-5p/CENPF axis. Biomedicine & Pharmacotherapy, 128, 110289.

Liang, M., Jia, J., Chen, L., Wei, B., Guan, Q., Ding, Z., ... & He, G. (2019). LncRNA MCM3AP-AS1 promotes proliferation and invasion through regulating miR-211-5p/SPARC axis in papillary thyroid cancer. Endocrine, 65, 318-326.

Wang, Y., Yang, L., Chen, T., Liu, X., Guo, Y., Zhu, Q., ... & Tu, K. (2019). A novel lncRNA MCM3AP-AS1 promotes the growth of hepatocellular carcinoma by targeting miR-194-5p/FOXA1 axis. Molecular cancer, 18(1), 1-16.

Zhang, H., Luo, C., & Zhang, G. (2019). LncRNA MCM3AP-AS1 regulates epidermal growth factor receptor and autophagy to promote hepatocellular carcinoma metastasis by interacting with miR-455. DNA and Cell Biology, 38(8), 857-864.

Yang, M., Sun, S., Guo, Y., Qin, J., & Liu, G. (2019). Long non-coding RNA MCM3AP-AS1 promotes growth and migration through modulating FOXK1 by sponging miR-138-5p in pancreatic cancer. Molecular Medicine, 25, 1-10.

Gao, Y., Zhao, H., & Li, Y. (2019). LncRNA MCM3AP-AS1 regulates miR-142-3p/HMGB1 to promote LPS-induced chondrocyte apoptosis. BMC musculoskeletal disorders, 20, 1-7.

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For research use only. Not intended for any clinical use.

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