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Rabbit Anti-MCU Recombinant Antibody (CBFYM-1931) (CBMAB-M2105-FY)

This product is rabbit antibody that recognizes MCU. The antibody CBFYM-1931 can be used for immunoassay techniques such as: WB, IP.
See all MCU antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
CBFYM-1931
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Mitochondrial Calcium Uniporter
Introduction
This gene encodes a calcium transporter that localizes to the mitochondrial inner membrane. The encoded protein interacts with mitochondrial calcium uptake 1. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
Human90550
Mouse215999
Rat294560
Monkey708334
UniProt ID
HumanQ8NE86
MouseQ3UMR5
RatM0RDI5
MonkeyF6U7W6
Alternative Names
Mitochondrial Calcium Uniporter; Coiled-Coil Domain-Containing Protein 109A; C10orf42; CCDC109A; HsMCU; Calcium Uniporter Protein, Mitochondrial; Coiled-Coil Domain Containing 109A
Function
Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria (PubMed:21685888, PubMed:21685886, PubMed:23101630, PubMed:22904319, PubMed:23178883, PubMed:22829870, PubMed:22822213, PubMed:24332854, PubMed:23755363, PubMed:26341627).

Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex) (PubMed:23755363).

Activity is regulated by MICU1 and MICU2. At low Ca2+ levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca2+ levels MICU1 increases MCU activity (PubMed:24560927, PubMed:26903221).

Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes (PubMed:22822213).

While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells (PubMed:25603276).

participates in mitochondrial permeability transition during ischemia-reperfusion injury (By similarity).

Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed:22904319, PubMed:22829870).

Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults (By similarity).

Regulates synaptic vesicle endocytosis kinetics in central nerve terminal (By similarity).

Involved in antigen processing and presentation (By similarity).
Biological Process
Actin filament reorganization Source: CACAO
Calcium import into the mitochondrion Source: MGI
Calcium-mediated signaling Source: UniProtKB
Glucose homeostasis Source: UniProtKB
Mitochondrial calcium ion homeostasis Source: ComplexPortal
Mitochondrial calcium ion transmembrane transport Source: UniProtKB
Positive regulation of insulin secretion Source: UniProtKB
Positive regulation of mitochondrial calcium ion concentration Source: UniProtKB
Positive regulation of mitochondrial fission Source: CACAO
Positive regulation of neutrophil chemotaxis Source: CACAO
Protein complex oligomerization Source: UniProtKB
Cellular Location
Mitochondrion inner membrane
Topology
Mitochondrial matrix: 51-233
Helical: 234-256
Mitochondrial intermembrane: 257-265
Helical: 266-283
Mitochondrial matrix: 284-351
PTM
Phosphorylation by CaMK2 in heart leads to increased MCU current (PubMed:23051746, PubMed:25254481). The regulation of MCU by CaMK2 is however subject to discussion: another group was unable to reproduce these results (PubMed:25254480). Phosphorylated on tyrosines by PTK2B/PYK2, promoting oligomerization (PubMed:24800979).

D’Angelo, D., & Rizzuto, R. (2023). The Mitochondrial Calcium Uniporter (MCU): Molecular Identity and Role in Human Diseases. Biomolecules, 13(9), 1304.

Weiser, A., Hermant, A., Bermont, F., Sizzano, F., Karaz, S., Alvarez-Illera, P., ... & De Marchi, U. (2023). The mitochondrial calcium uniporter (MCU) activates mitochondrial respiration and enhances mobility by regulating mitochondrial redox state. Redox Biology, 102759.

Yoo, J. (2022). Structural basis of Ca2+ uptake by mitochondrial calcium uniporter in mitochondria: a brief review. BMB reports, 55(11), 528.

Boyman, L., Greiser, M., & Lederer, W. J. (2021). Calcium influx through the mitochondrial calcium uniporter holocomplex, MCUcx. Journal of molecular and cellular cardiology, 151, 145-154.

Alevriadou, B. R., Patel, A., Noble, M., Ghosh, S., Gohil, V. M., Stathopulos, P. B., & Madesh, M. (2021). Molecular nature and physiological role of the mitochondrial calcium uniporter channel. American Journal of Physiology-Cell Physiology.

Bisbach, C. M., Hutto, R. A., Poria, D., Cleghorn, W. M., Abbas, F., Vinberg, F., ... & Brockerhoff, S. E. (2020). Mitochondrial Calcium Uniporter (MCU) deficiency reveals an alternate path for Ca2+ uptake in photoreceptor mitochondria. Scientific reports, 10(1), 16041.

Van Keuren, A. M., Tsai, C. W., Balderas, E., Rodriguez, M. X., Chaudhuri, D., & Tsai, M. F. (2020). Mechanisms of EMRE-dependent MCU opening in the mitochondrial calcium uniporter complex. Cell reports, 33(10).

Phillips, C. B., Tsai, C. W., & Tsai, M. F. (2019). The conserved aspartate ring of MCU mediates MICU1 binding and regulation in the mitochondrial calcium uniporter complex. Elife, 8, e41112.

Woods, J. J., Nemani, N., Shanmughapriya, S., Kumar, A., Zhang, M., Nathan, S. R., ... & Madesh, M. (2019). A selective and cell-permeable mitochondrial calcium uniporter (MCU) inhibitor preserves mitochondrial bioenergetics after hypoxia/reoxygenation injury. ACS central science, 5(1), 153-166.

Cui, C., Yang, J., Fu, L., Wang, M., & Wang, X. (2019). Progress in understanding mitochondrial calcium uniporter complex‐mediated calcium signalling: A potential target for cancer treatment. British journal of pharmacology, 176(9), 1190-1205.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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