Mouse Anti-MDM2 (N-terminus) Recombinant Antibody (CBFYM-1964) (CBMAB-M2139-FY)

MDM2 Proto-Oncogene

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

MDM2 Proto-Oncogene; MDM2 Proto-Oncogene, E3 Ubiquitin Protein Ligase; Oncoprotein Mdm2; Hdm2; Mdm2, Transformed 3T3 Cell Double Minute 2, P53 Binding Protein (Mouse); Mdm2, Transformed 3T3 Cell Double Minute 2, P53 Binding Protein; Mouse Double Minute 2, Human Homolog Of; P53-Binding Protein; Double Minute 2, Human Homolog Of; P53-Binding Protein; Mdm2, P53 E3 Ubiquitin Protein Ligase Homolog; MDM2 Oncogene, E3 Ubiquitin Protein Ligase

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911).

Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (By similarity).

Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis (By similarity).

Negatively regulates NDUFS1, leading to decreased mitochondrial respiration, marked oxidative stress, and commitment to the mitochondrial pathway of apoptosis (PubMed:30879903).

Binds NDUFS1 leading to its cytosolic retention rather than mitochondrial localization resulting in decreased supercomplex assembly (interactions between complex I and complex III), decreased complex I activity, ROS production, and apoptosis (PubMed:30879903).

Amyloid fibril formation Source: CAFA
Apoptotic process Source: UniProtKB
Atrial septum development Source: Ensembl
Atrioventricular valve morphogenesis Source: Ensembl
Blood vessel development Source: Ensembl
Blood vessel remodeling Source: Ensembl
Cardiac septum morphogenesis Source: Ensembl
Cellular response to actinomycin D Source: CAFA
Cellular response to gamma radiation Source: CAFA
Cellular response to hypoxia Source: UniProtKB
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: UniProtKB
Endocardial cushion morphogenesis Source: Ensembl
Establishment of protein localization Source: BHF-UCL
Negative regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
Negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator Source: CAFA
Negative regulation of signal transduction by p53 class mediator Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of cell population proliferation Source: BHF-UCL
Positive regulation of mitotic cell cycle Source: UniProtKB
Positive regulation of muscle cell differentiation Source: Ensembl
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: BHF-UCL
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: ARUK-UCL
Protein autoubiquitination Source: UniProtKB
Protein-containing complex assembly Source: UniProtKB
Protein destabilization Source: BHF-UCL
Protein localization to nucleus Source: BHF-UCL
Protein polyubiquitination Source: ARUK-UCL
Protein ubiquitination Source: UniProtKB
Proteolysis involved in cellular protein catabolic process Source: CAFA
Regulation of cell cycle Source: BHF-UCL
Regulation of heart rate Source: Ensembl
Regulation of protein catabolic process Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to antibiotic Source: UniProtKB
Transcription factor catabolic process Source: ParkinsonsUK-UCL
Traversing start control point of mitotic cell cycle Source: Ensembl
Ubiquitin-dependent protein catabolic process Source: UniProtKB
Ventricular septum development Source: Ensembl

Nucleus
nucleoplasm
nucleolus
Nucleus
Cytoplasm
Note: Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.

Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.
Lessel-Kubisch syndrome (LSKB):
An autosomal recessive progeroid syndrome characterized by short stature, pinched facial features, prematurely gray hair, scleroderma-like skin changes, small kidneys and consecutive kidney failure, followed by severe arterial hypertension.

Phosphorylation on Ser-166 by SGK1 activates ubiquitination of p53/TP53. Phosphorylated at multiple sites near the RING domain by ATM upon DNA damage; this prevents oligomerization and E3 ligase processivity and impedes constitutive p53/TP53 degradation.
Autoubiquitination leads to proteasomal degradation; resulting in p53/TP53 activation it may be regulated by SFN. Also ubiquitinated by TRIM13. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitination and degradation of p53/TP53. Deubiquitinated by USP7 leading to its stabilization.