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Mouse Anti-MERTK Recombinant Antibody (2D2) (CBMAB-A5433-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
2D2
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
MERTK (NP_006334, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
MER Proto-Oncogene, Tyrosine Kinase
Introduction
This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). [provided by RefSeq]
Entrez Gene ID
10461
UniProt ID
Q12866
Alternative Names
MER; MGC133349; RP38; c-mer
Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed:32640697).

Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
Biological Process
Cell-cell signaling Source: ProtInc
Cell migration Source: GO_Central
Cell surface receptor signaling pathway Source: ProtInc
Natural killer cell differentiation Source: Ensembl
Negative regulation of cytokine production Source: Ensembl
Negative regulation of leukocyte apoptotic process Source: CACAO
Negative regulation of lymphocyte activation Source: Ensembl
Nervous system development Source: GO_Central
Neutrophil clearance Source: Ensembl
Phagocytosis Source: BHF-UCL
Platelet activation Source: Ensembl
Positive regulation of kinase activity Source: GO_Central
Positive regulation of phagocytosis Source: UniProtKB
Protein kinase B signaling Source: Ensembl
Protein phosphorylation Source: ProtInc
Retina development in camera-type eye Source: Ensembl
Secretion by cell Source: Ensembl
Spermatogenesis Source: Ensembl
Substrate adhesion-dependent cell spreading Source: Ensembl
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Vagina development Source: Ensembl
Cellular Location
Cell membrane
Involvement in disease
Retinitis pigmentosa 38 (RP38):
A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Topology
Extracellular: 21-505
Helical: 506-526
Cytoplasmic: 527-999
PTM
Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2 (By similarity).
More Infomation

Engelmann, J., Zarrer, J., Gensch, V., Riecken, K., Berenbrok, N., Luu, T. V., ... & Loges, S. (2022). Regulation of bone homeostasis by MERTK and TYRO3. Nature Communications, 13(1), 7689.

Yan, D., Huelse, J. M., Kireev, D., Tan, Z., Chen, L., Goyal, S., ... & Graham, D. K. (2022). MERTK activation drives osimertinib resistance in EGFR-mutant non–small cell lung cancer. The Journal of Clinical Investigation, 132(15).

Chen, C. J., & Liu, Y. P. (2021). MERTK Inhibition: Potential as a treatment strategy in EGFR tyrosine kinase inhibitor-resistant non-small cell lung cancer. Pharmaceuticals, 14(2), 130.

Shen, K., Reichelt, M., Kyauk, R. V., Ngu, H., Shen, Y. A. A., Foreman, O., ... & Yuen, T. J. (2021). Multiple sclerosis risk gene Mertk is required for microglial activation and subsequent remyelination. Cell Reports, 34(10).

Tormoen, G. W., Blair, T. C., Bambina, S., Kramer, G., Baird, J., Rahmani, R., ... & Crittenden, M. (2020). Targeting MERTK enhances adaptive immune responses after radiation therapy. International Journal of Radiation Oncology* Biology* Physics, 108(1), 93-103.

Huelse, J. M., Fridlyand, D. M., Earp, S., DeRyckere, D., & Graham, D. K. (2020). MERTK in cancer therapy: targeting the receptor tyrosine kinase in tumor cells and the immune system. Pharmacology & therapeutics, 213, 107577.

Cai, B., Dongiovanni, P., Corey, K. E., Wang, X., Shmarakov, I. O., Zheng, Z., ... & Tabas, I. (2020). Macrophage MerTK promotes liver fibrosis in nonalcoholic steatohepatitis. Cell metabolism, 31(2), 406-421.

Peeters, M. J., Dulkeviciute, D., Draghi, A., Ritter, C., Rahbech, A., Skadborg, S. K., ... & thor Straten, P. (2019). MERTK acts as a costimulatory receptor on human CD8+ T cells. Cancer immunology research, 7(9), 1472-1484.

Myers, K. V., Amend, S. R., & Pienta, K. J. (2019). Targeting Tyro3, Axl and MerTK (TAM receptors): implications for macrophages in the tumor microenvironment. Molecular cancer, 18(1), 1-14.

Li, Y., Wittchen, E. S., Monaghan-Benson, E., Hahn, C., Earp, H. S., Doerschuk, C. M., & Burridge, K. (2019). The role of endothelial MERTK during the inflammatory response in lungs. PLoS One, 14(12), e0225051.

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For research use only. Not intended for any clinical use.

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