Mouse Anti-MID1 (AA 441-540) Recombinant Antibody (CBFYM-2218) (CBMAB-M2401-FY)

Name: Mouse Anti-MID1 (AA 441-540) Recombinant Antibody (CBFYM-2218)
SKU: CBMAB-M2401-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2218

Application

ELISA, WB

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: PNIKQNHYTV HGLQSGTKYI FMVKAINQAG SRSSEPGKLK TNSQPFKLDP KSAHRKLKVS HDNLTVERDE SSSKKSHTPE RFTSQGSYGV AGNVFIDSGR

Specificity

Human

Antibody Isotype

IgG2b, k

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 441-540

More Infomation

Target

Full Name

midline 1 (Opitz/BBB syndrome)

Introduction

The protein encoded by this gene is a member of the tripartite motif family, also known as the 'RING-B box-coiled coil' subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities.

Entrez Gene ID

4281

UniProt ID

O15344

Alternative Names

Midline 1; RING-Type E3 Ubiquitin Transferase Midline-1; Tripartite Motif-Containing Protein 18; Putative Transcription Factor XPRF; RING Finger Protein Midline-1; RING Finger Protein 59; Opitz/BBB Syndrome; TRIM18; MIDIN; RNF59; XPRF; FXY

Function

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.

Biological Process

Microtubule cytoskeleton organization Source: ProtInc
Negative regulation of microtubule depolymerization Source: Ensembl
Pattern specification process Source: ProtInc
Positive regulation of stress-activated MAPK cascade Source: UniProtKB
Protein localization to microtubule Source: UniProtKB

Cellular Location

Cytoplasm
Cytoskeleton
spindle
Note: Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.

Involvement in disease

Opitz GBBB syndrome 1 (GBBB1):
A congenital midline malformation syndrome characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, cleft lip/palate, laryngotracheoesophageal abnormalities, imperforate anus, developmental delay and congenital heart defects.

PTM

Phosphorylated on serine and threonine residues.

Girkin, J. L., Sokulsky, L. A., Starkey, M. R., Hansbro, P. M., Foster, P. S., Collison, A. M., & Mattes, J. (2023). A unique role for IL-13 in inducing esophageal eosinophilia through MID-1 and STAT6. Frontiers in allergy, 4.

Rao, X., Razavi, M., Mihai, G., Wei, Y., Braunstein, Z., Frieman, M. B., ... & Zhong, J. (2023). Dipeptidyl Peptidase 4/Midline‐1 Axis Promotes T Lymphocyte Motility in Atherosclerosis. Advanced Science, 10(9), 2204194.

Du, F., Hawez, A., Ding, Z., Wang, Y., Rönnow, C. F., Rahman, M., & Thorlacius, H. (2022). E3 Ubiquitin Ligase Midline 1 Regulates Endothelial Cell ICAM-1 Expression and Neutrophil Adhesion in Abdominal Sepsis. International Journal of Molecular Sciences, 24(1), 705.

Chen, X., Zhao, Q., Xu, Y., Wu, Q., Zhang, R., Du, Q., ... & Yuan, Y. (2022). E3 ubiquitin ligase MID1 ubiquitinates and degrades type‐I interferon receptor 2. Immunology, 167(3), 398-412.

Shen, S., Yang, Y., Shen, P., Ma, J., Fang, B., Wang, Q., ... & Fang, X. (2021). circPDE4B prevents articular cartilage degeneration and promotes repair by acting as a scaffold for RIC8A and MID1. Annals of the Rheumatic Diseases, 80(9), 1209-1219.

Chen, X., Xu, Y., Tu, W., Huang, F., Zuo, Y., Zhang, H. G., ... & Wang, J. (2021). Ubiquitin E3 ligase MID1 inhibits the innate immune response by ubiquitinating IRF3. Immunology, 163(3), 278-292.

Baldini, R., Mascaro, M., & Meroni, G. (2020). The MID1 gene product in physiology and disease. Gene, 747, 144655.

Zanchetta, M. E., & Meroni, G. (2019). Emerging roles of the TRIM E3 ubiquitin ligases MID1 and MID2 in cytokinesis. Frontiers in physiology, 10, 274.

Gohain, D., & Tamuli, R. (2019). Calcineurin responsive zinc‐finger‐1 binds to a unique promoter sequence to upregulate neuronal calcium sensor‐1, whose interaction with MID‐1 increases tolerance to calcium stress in Neurospora crassa. Molecular microbiology, 111(6), 1510-1528.

Collison, A. M., Li, J., De Siqueira, A. P., Lv, X., Toop, H. D., Morris, J. C., ... & Mattes, J. (2019). TRAIL signals through the ubiquitin ligase MID1 to promote pulmonary fibrosis. BMC Pulmonary Medicine, 19(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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