Mouse Anti-MPHOSPH9 Recombinant Antibody (CBFYM-2490) (CBMAB-M2677-FY)

Name: Mouse Anti-MPHOSPH9 Recombinant Antibody (CBFYM-2490)
SKU: CBMAB-M2677-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2490

Application

ELISA, IHC-P, WB

Specificity

Human

Antibody Isotype

IgG2a, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

M-phase phosphoprotein 9

Introduction

MPHOSPH9 is a Protein Coding gene.

Entrez Gene ID

10198

UniProt ID

Q99550

Alternative Names

M-Phase Phosphoprotein 9; MPP9; MPP-9

Function

Negatively regulates cilia formation by recruiting the CP110-CEP97 complex (a negative regulator of ciliogenesis) at the distal end of the mother centriole in ciliary cells (PubMed:30375385).

At the beginning of cilia formation, MPHOSPH9 undergoes TTBK2-mediated phosphorylation and degradation via the ubiquitin-proteasome system and removes itself and the CP110-CEP97 complex from the distal end of the mother centriole, which subsequently promotes cilia formation (PubMed:30375385).

Biological Process

Negative regulation of cilium assembly Source: UniProtKB

Cellular Location

Cytoskeleton
centriole
centrosome
Golgi apparatus
Golgi apparatus membrane
Note: Localizes to the distal and proximal end of centriole pairs in duplicated centrosomes. In ciliated cells, localizes to the distal and proximal end of daughter centriole and proximal of the mother centriole but not in the distal end of the mother centriole (PubMed:21399614). Recruited by KIF24 to the distal end of mother centriole where it forms a ring-like structure (PubMed:30375385).

PTM

TTBK2-mediated phosphorylation at Ser-629 and Ser-636, promotes its ubiquitination at Lys-632 leading to proteasomal degradation, loss of MPHOSPH9 facilitates the removal of the CP110-CEP97 complex from the mother centrioles, promoting the initiation of ciliogenesis (PubMed:30375385). Phosphorylated in M (mitotic) phase (PubMed:8885239).
Ubiquitinated at Lys-632, leading to proteasomal degradation.

Jiang, B., Liu, M., Li, P., Zhu, Y., Liu, Y., Zhu, K., ... & Li, Y. (2022). Rna-seq reveals a novel porcine lncrna mphosph9-ot1 induces cxcl8/il-8 expression in etec infected ipec-j2 cells. Frontiers in Cellular and Infection Microbiology, 12, 996841.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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