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Recombinant Mouse Anti-MUS81 Recombinant Antibody (2G10/3) (CBMAB-XB0855-YC)

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Datasheet
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
2G10/3
Application
FC, IHC, IF, IP, WB
Immunogen
Human MUS81
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
MUS81 Structure-Specific Endonuclease Subunit
Introduction
MUS81 is a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks.
Entrez Gene ID
80198
UniProt ID
Q96NY9
Alternative Names
MUS81 Structure-Specific Endonuclease Subunit; SLX3 Structure-Specific Endonuclease Subunit Homolog (S. Cerevisiae); SLX3 Structure-Specific Endonuclease Subunit Homolog; MUS81 Endonuclease Homolog (S. Cerevisiae); Crossover Junction Endonuclease MUS81; MUS81 Endonuclease Homolog (Yeast);
Function
Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. Plays an essential role in mitosis for the processing of stalled or collapsed replication forks (PubMed:28575661).
Biological Process
DNA catabolic process, endonucleolytic Source: MGI
DNA repair Source: UniProtKB
Double-strand break repair Source: ComplexPortal
Double-strand break repair via break-induced replication Source: GO_Central
Mitotic intra-S DNA damage checkpoint signaling Source: GO_Central
Osteoblast proliferation Source: CACAO
Replication fork processing Source: ComplexPortal
Resolution of meiotic recombination intermediates Source: GO_Central
Response to intra-S DNA damage checkpoint signaling Source: MGI
Cellular Location
Nucleolus
Note: Recruited to foci of DNA damage in S-phase cells.

Marini, V., Nikulenkov, F., Samadder, P., Juul, S., Knudsen, B. R., & Krejci, L. (2023). MUS81 cleaves TOP1-derived lesions and other DNA–protein cross-links. BMC biology, 21(1), 110.

Payliss, B. J., Tse, Y. W. E., Reichheld, S. E., Lemak, A., Yun, H. Y., Houliston, S., ... & Wyatt, H. D. (2022). Phosphorylation of the DNA repair scaffold SLX4 drives folding of the SAP domain and activation of the MUS81-EME1 endonuclease. Cell Reports, 41(4).

Hua, Z., Fang, Q., Zhang, D., Luo, Z., Yuan, C., & Lin, Z. (2022). Crystal structure of the human MUS81-EME2 complex. Structure, 30(5), 743-752.

Giaccherini, C., Scaglione, S., Coulon, S., Dehé, P. M., & Gaillard, P. H. L. (2022). Regulation of Mus81-Eme1 structure-specific endonuclease by Eme1 SUMO-binding and Rad3ATR kinase is essential in the absence of Rqh1BLM helicase. PLoS Genetics, 18(4), e1010165.

Giaccherini, C., & Gaillard, P. H. L. (2021). Control of structure-specific endonucleases during homologous recombination in eukaryotes. Current Opinion in Genetics & Development, 71, 195-205.

Xu, X., Wang, M., Sun, J., Yu, Z., Li, G., Yang, N., & Xu, R. M. (2021). Structure specific DNA recognition by the SLX1–SLX4 endonuclease complex. Nucleic Acids Research, 49(13), 7740-7752.

Waizenegger, A., Urulangodi, M., Lehmann, C. P., Reyes, T. A. C., Saugar, I., Tercero, J. A., ... & Branzei, D. (2020). Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity. Nature Communications, 11(1), 5746.

Pardo, B., Moriel-Carretero, M., Vicat, T., Aguilera, A., & Pasero, P. (2019). A new role of the Mus81 nuclease for replication completion after fork restart. bioRxiv, 785501.

Falquet, B., & Rass, U. (2019). Structure-specific endonucleases and the resolution of chromosome underreplication. Genes, 10(3), 232.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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