Mouse Anti-MUTYH (AA 436-535) Recombinant Antibody (CBFYM-2837) (CBMAB-M3030-FY)

Name: Mouse Anti-MUTYH (AA 436-535) Recombinant Antibody (CBFYM-2837)
SKU: CBMAB-M3030-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2837

Application

ELISA, IF, WB

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: KLTYQVYGLA LEGQTPVTTV PPGARWLTQE EFHTAAVSTA MKKVFRVYQG QQPGTCMGSK RSQVSSPCSR KKPRMGQQVL DNFFRSHIST DAHSLNSAAQ

Specificity

Human

Antibody Isotype

IgG1, k

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 436-535

More Infomation

Target

Full Name

MutY DNA Glycosylase

Introduction

This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. This gene product is thought to play a role in signaling apoptosis by the introduction of single-strand breaks following oxidative damage. Mutations in this gene result in heritable predisposition to colorectal cancer, termed MUTYH-associated polyposis. Multiple transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

4595

UniProt ID

Q9UIF7

Alternative Names

MutY DNA Glycosylase; MutY Homolog; MYH; A/G-Specific Adenine DNA Glycosylase; Adenine DNA Glycosylase; MutY Homolog (E. Coli)

Function

Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.

Biological Process

Base-excision repair Source: GO_Central
Depurination Source: Reactome
DNA repair Source: ProtInc
Mismatch repair Source: GO_Central

Cellular Location

Mitochondrion
Nucleus

Involvement in disease

Familial adenomatous polyposis 2 (FAP2):
A condition characterized by the development of multiple colorectal adenomatous polyps, benign neoplasms derived from glandular epithelium. Some affected individuals may develop colorectal carcinoma.
Gastric cancer (GASC):
A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.

Robinson, P. S., Thomas, L. E., Abascal, F., Jung, H., Harvey, L. M., West, H. D., ... & Stratton, M. R. (2022). Inherited MUTYH mutations cause elevated somatic mutation rates and distinctive mutational signatures in normal human cells. Nature Communications, 13(1), 3949.

Magrin, L., Fanale, D., Brando, C., Corsini, L. R., Randazzo, U., Di Piazza, M., ... & Bazan, V. (2022). MUTYH-associated tumor syndrome: The other face of MAP. Oncogene, 41(18), 2531-2539.

Barreiro, R. A. S., Sabbaga, J., Rossi, B. M., Achatz, M. I. W., Bettoni, F., Camargo, A. A., ... & AF Galante, P. (2022). Monoallelic deleterious MUTYH germline variants as a driver for tumorigenesis. The Journal of Pathology, 256(2), 214-222.

Mizuno, Y., Abolhassani, N., Mazzei, G., Sakumi, K., Saito, T., Saido, T. C., ... & Nakabeppu, Y. (2021). MUTYH actively contributes to microglial activation and impaired neurogenesis in the pathogenesis of Alzheimer’s disease. Oxidative medicine and cellular longevity, 2021.

Nielsen, M., Infante, E., & Brand, R. (2021). MUTYH polyposis.

Hutchcraft, M. L., Gallion, H. H., & Kolesar, J. M. (2021). MUTYH as an emerging predictive biomarker in ovarian cancer. Diagnostics, 11(1), 84.

Jang, S., Schaich, M. A., Khuu, C., Schnable, B. L., Majumdar, C., Watkins, S. C., ... & Van Houten, B. (2021). Single molecule analysis indicates stimulation of MUTYH by UV-DDB through enzyme turnover. Nucleic Acids Research, 49(14), 8177-8188.

Nakamura, T., Okabe, K., Hirayama, S., Chirifu, M., Ikemizu, S., Morioka, H., ... & Yamagata, Y. (2021). Structure of the mammalian adenine DNA glycosylase MUTYH: insights into the base excision repair pathway and cancer. Nucleic acids research, 49(12), 7154-7163.

Curia, M. C., Catalano, T., & Aceto, G. M. (2020). MUTYH: Not just polyposis. World Journal of Clinical Oncology, 11(7), 428.

Raetz, A. G., & David, S. S. (2019). When you’re strange: Unusual features of the MUTYH glycosylase and implications in cancer. DNA repair, 80, 16-25.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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