Sign in or Register   Sign in or Register
  |  

Rabbit Anti-NPC1L1 Recombinant Antibody (CBWJN-1013) (CBMAB-N0486-WJ)

This product is a Rabbit antibody that recognizes NPC1L1. The antibody CBWJN-1013 can be used for immunoassay techniques such as: WB, IHC-P, ICC.
See all NPC1L1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBWJN-1013
Antibody Isotype
IgG
Application
WB, IHC-P, ICC

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NPC1 Like Intracellular Cholesterol Transporter 1
Introduction
The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Entrez Gene ID
29881
UniProt ID
Q9UHC9
Alternative Names
NPC1 Like Intracellular Cholesterol Transporter 1; NPC1 (Niemann-Pick Disease, Type C1, Gene)-Like 1; NPC1 Like 1; Niemann-Pick C1-Like Protein 1; NPC1-Like 1; NPC11L1; SLC65A2;
Function
Plays a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorption. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. Acts as a negative regulator of NPC2 and down-regulates its expression and secretion by inhibiting its maturation and accelerating its degradation.
Biological Process
Cellular response to sterol depletionManual Assertion Based On ExperimentIDA:UniProtKB
Cholesterol biosynthetic processManual Assertion Based On ExperimentIMP:HGNC-UCL
Cholesterol transportManual Assertion Based On ExperimentIMP:HGNC-UCL
Intestinal cholesterol absorptionManual Assertion Based On ExperimentIMP:HGNC-UCL
Lipoprotein metabolic processManual Assertion Based On ExperimentIMP:HGNC-UCL
Cellular Location
Apical cell membrane
Cell membrane
Cytoplasmic vesicle membrane
Subfractionation of brush border membranes from proximal enterocytes suggests considerable association with the apical membrane fraction. Exists as a predominantly cell surface membrane expressed protein (By similarity).
According to PubMed:15671032, localizes in a subcellular vesicular compartment rich in RAB5.
Topology
Extracellular: 22-284
Helical: 285-305
Cytoplasmic: 306-351
Helical: 352-372
Extracellular: 373-632
Helical: 633-653
Cytoplasmic: 654-666
Helical: 667-687
Extracellular: 688-696
Helical: 697-717
Cytoplasmic: 718-742
Helical: 743-763
Extracellular: 764-776
Helical: 777-797
Cytoplasmic: 798-846
Helical: 847-867
Extracellular: 868-1139
Helical: 1140-1160
Cytoplasmic: 1161-1168
Helical: 1169-1189
Extracellular: 1190-1191
Helical: 1192-1212
Cytoplasmic: 1213-1236
Helical: 1237-1257
Extracellular: 1258-1268
Helical: 1269-1289
Cytoplasmic: 1290-1359
PTM
Highly glycosylated.

Xiao, J., Dong, L. W., Liu, S., Meng, F. H., Xie, C., Lu, X. Y., ... & Song, B. L. (2023). Bile acids-mediated intracellular cholesterol transport promotes intestinal cholesterol absorption and NPC1L1 recycling. Nature Communications, 14(1), 6469.

Zhang, R., Liu, W., Zeng, J., Meng, J., Shi, L., Yang, S., ... & Xing, D. (2022). Recent advances in the screening methods of NPC1L1 inhibitors. Biomedicine & Pharmacotherapy, 155, 113732.

Liu, W., Liang, B., Zeng, J., Meng, J., Shi, L., Yang, S., ... & Xing, D. (2022). First Discovery of Cholesterol-Lowering Activity of Parthenolide as NPC1L1 Inhibitor. Molecules, 27(19), 6270.

Hu, M., Yang, F., Huang, Y., You, X., Liu, D., Sun, S., & Sui, S. F. (2021). Structural insights into the mechanism of human NPC1L1-mediated cholesterol uptake. Science Advances, 7(29), eabg3188.

Long, T., Liu, Y., Qin, Y., DeBose-Boyd, R. A., & Li, X. (2021). Structures of dimeric human NPC1L1 provide insight into mechanisms for cholesterol absorption. Science Advances, 7(34), eabh3997.

Saha, P., Shumate, J. L., Caldwell, J. G., Elghobashi-Meinhardt, N., Lu, A., Zhang, L., ... & Pfeffer, S. R. (2020). Inter-domain dynamics drive cholesterol transport by NPC1 and NPC1L1 proteins. Elife, 9, e57089.

Huang, C. S., Yu, X., Fordstrom, P., Choi, K., Chung, B. C., Roh, S. H., ... & Wang, Z. (2020). Cryo-EM structures of NPC1L1 reveal mechanisms of cholesterol transport and ezetimibe inhibition. Science advances, 6(25), eabb1989.

Yamanashi, Y., Takada, T., Yamamoto, H., & Suzuki, H. (2020). NPC1L1 facilitates sphingomyelin absorption and regulates diet-induced production of VLDL/LDL-associated S1P. Nutrients, 12(9), 2641.

Toyoda, Y., Takada, T., Umezawa, M., Tomura, F., Yamanashi, Y., Takeda, K., & Suzuki, H. (2019). Identification of hepatic NPC1L1 as an NAFLD risk factor evidenced by ezetimibe‐mediated steatosis prevention and recovery. FASEB BioAdvances, 1(5), 283-295.

Kim, Y. C., Byun, S., Seok, S., Guo, G., Xu, H. E., Kemper, B., & Kemper, J. K. (2019). Small heterodimer partner and fibroblast growth factor 19 inhibit expression of NPC1L1 in mouse intestine and cholesterol absorption. Gastroenterology, 156(4), 1052-1065.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-NPC1L1 Recombinant Antibody (CBWJN-1013)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare