Rabbit Anti-NPC1L1 Recombinant Antibody (CBWJN-1013) (CBMAB-N0486-WJ)

Name: Rabbit Anti-NPC1L1 Recombinant Antibody (CBWJN-1013)
SKU: CBMAB-N0486-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBWJN-1013

Application

WB, IHC-P, ICC

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NPC1 Like Intracellular Cholesterol Transporter 1

Introduction

The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Entrez Gene ID

29881

UniProt ID

Q9UHC9

Alternative Names

NPC1 Like Intracellular Cholesterol Transporter 1; NPC1 (Niemann-Pick Disease, Type C1, Gene)-Like 1; NPC1 Like 1; Niemann-Pick C1-Like Protein 1; NPC1-Like 1; NPC11L1; SLC65A2;

Function

Plays a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorption. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. Acts as a negative regulator of NPC2 and down-regulates its expression and secretion by inhibiting its maturation and accelerating its degradation.

Biological Process

Cellular response to sterol depletionManual Assertion Based On ExperimentIDA:UniProtKB
Cholesterol biosynthetic processManual Assertion Based On ExperimentIMP:HGNC-UCL
Cholesterol transportManual Assertion Based On ExperimentIMP:HGNC-UCL
Intestinal cholesterol absorptionManual Assertion Based On ExperimentIMP:HGNC-UCL
Lipoprotein metabolic processManual Assertion Based On ExperimentIMP:HGNC-UCL

Cellular Location

Apical cell membrane
Cell membrane
Cytoplasmic vesicle membrane
Subfractionation of brush border membranes from proximal enterocytes suggests considerable association with the apical membrane fraction. Exists as a predominantly cell surface membrane expressed protein (By similarity).
According to PubMed:15671032, localizes in a subcellular vesicular compartment rich in RAB5.

Topology

Extracellular: 22-284
Helical: 285-305
Cytoplasmic: 306-351
Helical: 352-372
Extracellular: 373-632
Helical: 633-653
Cytoplasmic: 654-666
Helical: 667-687
Extracellular: 688-696
Helical: 697-717
Cytoplasmic: 718-742
Helical: 743-763
Extracellular: 764-776
Helical: 777-797
Cytoplasmic: 798-846
Helical: 847-867
Extracellular: 868-1139
Helical: 1140-1160
Cytoplasmic: 1161-1168
Helical: 1169-1189
Extracellular: 1190-1191
Helical: 1192-1212
Cytoplasmic: 1213-1236
Helical: 1237-1257
Extracellular: 1258-1268
Helical: 1269-1289
Cytoplasmic: 1290-1359

PTM

Highly glycosylated.

Xiao, J., Dong, L. W., Liu, S., Meng, F. H., Xie, C., Lu, X. Y., ... & Song, B. L. (2023). Bile acids-mediated intracellular cholesterol transport promotes intestinal cholesterol absorption and NPC1L1 recycling. Nature Communications, 14(1), 6469.

Zhang, R., Liu, W., Zeng, J., Meng, J., Shi, L., Yang, S., ... & Xing, D. (2022). Recent advances in the screening methods of NPC1L1 inhibitors. Biomedicine & Pharmacotherapy, 155, 113732.

Liu, W., Liang, B., Zeng, J., Meng, J., Shi, L., Yang, S., ... & Xing, D. (2022). First Discovery of Cholesterol-Lowering Activity of Parthenolide as NPC1L1 Inhibitor. Molecules, 27(19), 6270.

Hu, M., Yang, F., Huang, Y., You, X., Liu, D., Sun, S., & Sui, S. F. (2021). Structural insights into the mechanism of human NPC1L1-mediated cholesterol uptake. Science Advances, 7(29), eabg3188.

Long, T., Liu, Y., Qin, Y., DeBose-Boyd, R. A., & Li, X. (2021). Structures of dimeric human NPC1L1 provide insight into mechanisms for cholesterol absorption. Science Advances, 7(34), eabh3997.

Saha, P., Shumate, J. L., Caldwell, J. G., Elghobashi-Meinhardt, N., Lu, A., Zhang, L., ... & Pfeffer, S. R. (2020). Inter-domain dynamics drive cholesterol transport by NPC1 and NPC1L1 proteins. Elife, 9, e57089.

Huang, C. S., Yu, X., Fordstrom, P., Choi, K., Chung, B. C., Roh, S. H., ... & Wang, Z. (2020). Cryo-EM structures of NPC1L1 reveal mechanisms of cholesterol transport and ezetimibe inhibition. Science advances, 6(25), eabb1989.

Yamanashi, Y., Takada, T., Yamamoto, H., & Suzuki, H. (2020). NPC1L1 facilitates sphingomyelin absorption and regulates diet-induced production of VLDL/LDL-associated S1P. Nutrients, 12(9), 2641.

Toyoda, Y., Takada, T., Umezawa, M., Tomura, F., Yamanashi, Y., Takeda, K., & Suzuki, H. (2019). Identification of hepatic NPC1L1 as an NAFLD risk factor evidenced by ezetimibe‐mediated steatosis prevention and recovery. FASEB BioAdvances, 1(5), 283-295.

Kim, Y. C., Byun, S., Seok, S., Guo, G., Xu, H. E., Kemper, B., & Kemper, J. K. (2019). Small heterodimer partner and fibroblast growth factor 19 inhibit expression of NPC1L1 in mouse intestine and cholesterol absorption. Gastroenterology, 156(4), 1052-1065.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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