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Mouse Anti-NSMCE2 Recombinant Antibody (17D10) (CBMAB-N0544-WJ)

This product is a Mouse antibody that recognizes NSMCE2. The antibody 17D10 can be used for immunoassay techniques such as: WB, FC, ELISA, IF.
See all NSMCE2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
17D10
Antibody Isotype
IgG2b, κ
Application
WB, FC, ELISA, IF

Basic Information

Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NSE2/MMS21 HOMOLOG, SMC5-SMC6 COMPLEX SUMO LIGASE
Introduction
This gene encodes a member of a family of E3 small ubiquitin-related modifier (SUMO) ligases that mediates the attachment of a SUMO protein to proteins involved in nuclear transport, transcription, chromosome segregation and DNA repair. The encoded protein is part of the structural maintenance of chromosomes (SMC) 5/6 complex which plays a key role genome maintenance, facilitating chromosome segregation and suppressing mitotic recombination. A knockout of the orthologous mouse gene is lethal prior to embryonic day 10.5. Naturally occurring mutations in this gene, that abolish the SUMO ligase activity, are associated with primordial dwarfism and extreme insulin resistance. [provided by RefSeq, Mar 2017]
Entrez Gene ID
286053
UniProt ID
Q96MF7
Alternative Names
NSE2/MMS21 Homolog, SMC5-SMC6 Complex SUMO Ligase; Non-Structural Maintenance Of Chromosomes Element 2 Homolog; Zinc Finger, MIZ-Type Containing 7; C8orf36; MMS21; Non-SMC Element 2 Homolog (MMS21, S. Cerevisiae); Non-SMC Element 2, MMS21 Homolog (S. Cerevisiae); Methyl Methanesulfonate Sensitivity Gene 21; Chromosome 8 Open Reading Frame 36; Non-SMC Element 2, MMS21 Homolog; E3 SUMO-Protein Ligase NSE2;
Function
E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination (PubMed:16055714, PubMed:16810316).
Is not be required for the stability of the complex (PubMed:16055714, PubMed:16810316).
The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks (PubMed:16055714, PubMed:16810316).
The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PubMed:17589526).
Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TSNAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, RAD51AP1, and maybe the cohesin components RAD21 and STAG2 (PubMed:16055714, PubMed:16810316, PubMed:17589526, PubMed:31400850).
Required for recruitment of telomeres to PML nuclear bodies (PubMed:17589526).
SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines (PubMed:17589526).
Required for sister chromatid cohesion during prometaphase and mitotic progression (PubMed:19502785).
Biological Process
Cell cycleIEA:UniProtKB-KW
Cell divisionIEA:UniProtKB-KW
Cellular senescenceManual Assertion Based On ExperimentIMP:UniProtKB
Double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of maintenance of mitotic sister chromatid cohesionManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of mitotic metaphase/anaphase transitionManual Assertion Based On ExperimentIMP:UniProtKB
Protein sumoylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of telomere maintenance1 PublicationIC:ComplexPortal
Telomere maintenance via recombinationManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
Chromosome, telomere
Nucleus, PML body
Localizes to PML nuclear bodies in ALT cell lines.
Involvement in disease
Seckel syndrome 10 (SCKL10):
A form of Seckel syndrome, a rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation.
PTM
Sumoylated, possibly via autosumoylation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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