Rabbit Anti-NUMA1 Recombinant Antibody (CBWJN-0831) (CBMAB-N0561-WJ)

Rabbit

Mouse, Rat, Human

CBWJN-0831

IgG

WB, IHC-P, ICC

Mouse, Rat, Human

IgG

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Nuclear Mitotic Apparatus Protein 1

This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Nuclear Mitotic Apparatus Protein 1; Nuclear Matrix Protein-22; SP-H Antigen; NMP-22; NUMA; Centrophilin Stabilizes Mitotic Spindle In Mitotic Cells;

Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PubMed:7769006, PubMed:17172455, PubMed:19255246, PubMed:24996901, PubMed:26195665, PubMed:27462074).
Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PubMed:12445386, PubMed:11956313).
Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PubMed:23870127, PubMed:24109598, PubMed:24996901, PubMed:26765568).
In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle (PubMed:23027904, PubMed:22327364, PubMed:23921553).
During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation (PubMed:22327364, PubMed:23921553, PubMed:24996901, PubMed:24371089).
Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro (PubMed:24996901, PubMed:24371089).
Also required for proper orientation of the mitotic spindle during asymmetric cell divisions (PubMed:21816348).
Plays a role in mitotic MT aster assembly (PubMed:11163243, PubMed:11229403, PubMed:12445386).
Involved in anastral spindle assembly (PubMed:25657325).
Positively regulates TNKS protein localization to spindle poles in mitosis (PubMed:16076287).
Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume (PubMed:10075938).
Required for epidermal differentiation and hair follicle morphogenesis (By similarity).

Anastral spindle assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Astral microtubule organizationManual Assertion Based On ExperimentIDA:UniProtKB
Cell divisionIEA:UniProtKB-KW
Chromosome segregationIEA:UniProtKB-KW
Establishment of mitotic spindle orientationManual Assertion Based On ExperimentIDA:UniProtKB
Meiotic cell cycleIEA:Ensembl
Microtubule bundle formationManual Assertion Based On ExperimentIMP:UniProtKB
Nucleus organizationManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of BMP signaling pathwayISS:UniProtKB
Positive regulation of chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of chromosome separationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of hair follicle developmentISS:UniProtKB
Positive regulation of intracellular transportManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of keratinocyte differentiationISS:UniProtKB
Positive regulation of microtubule polymerizationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of mitotic spindle elongationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of protein localization to cell cortexManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of protein localization to spindle pole bodyManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of spindle assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of metaphase plate congressionManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of mitotic spindle organizationManual Assertion Based On ExperimentIDA:UniProtKB

Nucleus
Nucleus, nucleoplasm
Nucleus matrix
Chromosome
Cytoplasm, cytoskeleton
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm, cytoskeleton, spindle pole
Cytoplasm, cell cortex
Cell membrane
Lateral cell membrane
Mitotic cell cycle-dependent shuttling protein that relocalizes from the interphase nucleus to the spindle poles and cell cortex (PubMed:1541636, PubMed:10811826).
The localization to the spindle poles is regulated by AAAS (PubMed:26246606).
In interphase, resides in the nuclear matrix (PubMed:1541630, PubMed:1541636, PubMed:23921553).
In prophase, restricted to the interchromatin or condensed chromosome space (PubMed:10811826).
In prometaphase, after nuclear envelope disassembly, forms aggregates both in the spindle midzone and at duplicated centrosomes and astral microtubules (MTs) of the bipolar spindle apparatus (PubMed:10811826).
Translocates from the spindle midzone towards the spindle poles along spindle fibers in a MT- and dynein-dynactin-dependent manner until the anaphase onset (PubMed:1541636, PubMed:10811826).
In metaphase, recruited to the polar cortical region in a GPSM2- and GNAI1-dependent manner (PubMed:23870127, PubMed:24109598, PubMed:24996901).
Excluded from the metaphase equatorial cortical region in a RanGTP-dependent manner (PubMed:22327364, PubMed:23870127).
Phosphorylation on Thr-2055 by CDK1 results in its localization at spindle poles in metaphase, but not at the cell cortex (PubMed:23921553).
In anaphase, recruited and anchored at the cell membrane of the polar cortical region in a EPB41-, EPB41L2-, phosphatidylinositol-dependent and GPSM2- and G(i) alpha proteins-independent manner (PubMed:23870127, PubMed:24996901, PubMed:24109598, PubMed:24371089).
Excluded from the anaphase equatorial region of the cell cortex in a RACGAP1- and KIF23-dependent and RanGTP-independent manner (PubMed:24996901).
Associated with astral MTs emanating from the spindle poles during anaphase (PubMed:12445386, PubMed:24996901).
Nonphosphorylated Thr-2055 localizes at the cell cortex, weakly during metaphase and more prominently during anaphase in a phosphatase PPP2CA-dependent manner (PubMed:23921553).
As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident (PubMed:1541636).
Localizes to the tips of cortical MTs in prometaphase (PubMed:26765568).
Localizes along MTs and specifically to both MT plus and minus ends (PubMed:26765568).
Accumulates also at MT tips near the cell periphery (PubMed:26765568).
Colocalizes with GPSM2 at mitotic spindle poles during mitosis (PubMed:11781568, PubMed:21816348).
Colocalizes with SPAG5 at mitotic spindle at prometaphase and at mitotic spindle poles at metaphase and anaphase (PubMed:27462074).
Colocalizes with ABRO1 at mitotic spindle poles (PubMed:26195665).
Colocalized with TNKS from prophase through to anaphase in mitosis (PubMed:16076287).
Colocalizes with tubulin alpha (PubMed:12445386).
CCSAP is essential for its centrosomal localization (PubMed:26562023).
In horizontally retinal progenitor dividing cells, localized to the lateral cortical region (By similarity).
Isoform 3
Cytoplasm, cytosol
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm, cytoskeleton, spindle pole
During interphase, mainly clustered at the centrosomal region in the cytosol. After entry into mitosis, detected at mitotic spindle poles.
Isoform 4
Cytoplasm, cytosol
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm, cytoskeleton, spindle pole
During interphase, mainly clustered at the centrosomal region in the cytosol. After entry into mitosis, detected at mitotic spindle poles.

Phosphorylation and dephosphorylation on Thr-2055 regulates the extent of cortical NUMA1 and the dynein-dynactin complex localization during mitotic metaphase and anaphase (PubMed:23921553).
In metaphase, phosphorylation on Thr-2055 ^oCcurs in a kinase CDK1-dependent manner; this phosphorylation maintains low levels of cortical dynein-dynactin complex at metaphase, and hence proper spindle positioning (PubMed:7769006, PubMed:23921553, PubMed:24371089).
In anaphase, dephosphorylated on Thr-2055 by phosphatase PPP2CA; this dephosphorylation stimulates its membrane association and with the dynein-dynactin complex its enrichment at the cell cortex, and hence robust spindle elongation (PubMed:23921553, PubMed:24371089).
Probably also phosphorylated on Thr-2015 and Ser-2087 by CDK1; these phosphorylations may regulate its cell cortex recruitment during metaphase and anaphase (PubMed:23870127).
Phosphorylated on Thr-1047, Ser-1769, Ser-1772, Ser-1789 and Ser-1834 by PLK1; these phosphorylations induce cortical dynein-dynactin complex dissociation from the NUMA1-GPSM2 complex and negatively regulates cortical dynein-dynactin complex localization (PubMed:22327364).
ADP-ribosylated by TNKS at the onset of mitosis; ADP-ribosylation is not required for its localization to spindle poles (PubMed:16076287).
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status (PubMed:20068230).
Ubiquitinated with 'Lys-63'-linked polyubiquitin chains. Deubiquitination by the BRISC complex is important for the incorporation of NUMA1 into mitotic spindle poles and normal spindle pole function, probably by modulating interactions between NUMA1, dynein-dynactin complex and importin-beta.