Mouse Anti-NUP133 Recombinant Antibody (4F18) (CBMAB-N3985-WJ)

Name: Mouse Anti-NUP133 Recombinant Antibody (4F18)
SKU: CBMAB-N3985-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4F18

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

nucleoporin 133kDa

Introduction

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells. [provided by RefSeq, Jul 2008]

Entrez Gene ID

55746

UniProt ID

Q8WUM0

Alternative Names

Nucleoporin 133; 133 KDa Nucleoporin; Nucleoporin 133kDa; Nucleoporin Nup133; Nuclear Pore Complex Protein Nup133; Nucleoporin 133kD; HNUP133;

Function

Involved in poly(A)+ RNA transport. Involved in nephrogenesis (PubMed:30179222).

Biological Process

mRNA export from nucleusManual Assertion Based On ExperimentIDA:UniProtKB
Nephron developmentManual Assertion Based On ExperimentIMP:UniProtKB
Neural tube developmentIEA:Ensembl
NeurogenesisIEA:Ensembl
Nuclear pore organizationManual Assertion Based On ExperimentIMP:UniProtKB
Nucleocytoplasmic transport1 PublicationIC:ComplexPortal
Paraxial mesoderm developmentIEA:Ensembl
Poly(A)+ mRNA export from nucleusManual Assertion Based On ExperimentIBA:GO_Central
Protein import into nucleusManual Assertion Based On ExperimentIBA:GO_Central
Somite developmentIEA:Ensembl
Transcription-dependent tethering of RNA polymerase II gene DNA at nuclear peripheryManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus, nuclear pore complex
Chromosome, centromere, kinetochore
Located on both the cytoplasmic and nuclear sides of the nuclear pore (PubMed:11564755).
During mitosis, localizes to the kinetochores (PubMed:11564755).

Involvement in disease

Nephrotic syndrome 18 (NPHS18):
A form of nephrotic syndrome, a renal disease clinically characterized by severe proteinuria, resulting in complications such as hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form that progresses to end-stage renal failure. NPHS18 is an autosomal recessive, steroid-resistant progressive form with onset in the first decade of life.
Galloway-Mowat syndrome 8 (GAMOS8):
A form of Galloway-Mowat syndrome, a severe renal-neurological disease characterized by early-onset nephrotic syndrome associated with microcephaly, central nervous system abnormalities, developmental delays, and a propensity for seizures. Brain anomalies include gyration defects ranging from lissencephaly to pachygyria and polymicrogyria, and cerebellar hypoplasia. Most patients show facial dysmorphism characterized by a small, narrow forehead, large/floppy ears, deep-set eyes, hypertelorism and micrognathia. Additional variable features are visual impairment and arachnodactyly. Most patients die in early childhood. GAMOS8 inheritance is autosomal recessive.

Rogg, M., Maier, J. I., Ehle, M., Sammarco, A., Schilling, O., Werner, M., & Schell, C. (2022). NUP133 controls nuclear pore assembly, transcriptome composition, and cytoskeleton regulation in podocytes. Cells, 11(8), 1259.

Nordeen, S. A., Turman, D. L., & Schwartz, T. U. (2020). Structure of the yeast Nup84-Nup133 complex details flexibility and reveals universal conservation of the membrane anchoring ALPS motif. bioRxiv, 2020-06.

Nordeen, S. A., Turman, D. L., & Schwartz, T. U. (2020). Yeast Nup84-Nup133 complex structure details flexibility and reveals conservation of the membrane anchoring ALPS motif. Nature communications, 11(1), 6060.

Sunny, D. E., Hammer, E., Strempel, S., Joseph, C., Manchanda, H., Ittermann, T., ... & Heckmann, M. (2020). Nup133 and ERα mediate the differential effects of hyperoxia-induced damage in male and female OPCs. Molecular and cellular pediatrics, 7, 1-14.

Bilir, Ş., Kojidani, T., Mori, C., Osakada, H., Kobayashi, S., Koujin, T., ... & Haraguchi, T. (2019). Roles of Nup133, Nup153 and membrane fenestrations in assembly of the nuclear pore complex at the end of mitosis. Genes to cells, 24(5), 338-353.

Hammel, P., & Lima, W. C. (2019). RB436, RB437 and RB439 antibodies recognize a Dictyostelium Nup133 peptide by ELISA. [TEST] Antibody Reports, e13-e13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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