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Mouse Anti-PABPC1 Recombinant Antibody (10E10) (CBMAB-P0625-YC)

Provided herein is a Mouse monoclonal antibody against Human Poly(A) Binding Protein Cytoplasmic 1. The antibody can be used for immunoassay techniques, such as ELISA, ICC, IF, IP, WB.
See all PABPC1 antibodies
Published Data
Fig.1 Interaction between TAP‐YB‐1 and the C1QBP, DAP3, HNRPK, HNRPL, PABPC1, RALYL, RIBC2, RPL31, RPS7, RPS4X, and SARG proteins.
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Fig.2 PRMT4-dependent arginine methylation is not required for the association of PABP1 with translation complexes or regulation of its subcellular localization.
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Fig.3 PABP1 is arginine dimethylated independently of PRMT4.
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Fig.4 Both PABPN1 and PABPC1 bind to ORF1p through L1 RNA.
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Fig.5 Indirect immunofluorescence of pRB1B5-infected CEF 72 h posttransfection.
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Summary

Host Animal
Mouse
Specificity
Human, Chicken, Rabbit, Frog
Clone
10E10
Antibody Isotype
IgG2b
Application
ELISA, ICC, IF, IP, WB

Basic Information

Immunogen
Recombinant PABP (Human) expressed from its 1.85 kbp cDNA
Specificity
Human, Chicken, Rabbit, Frog
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, 0.1% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Poly(A) Binding Protein Cytoplasmic 1
Introduction
PABPC1 is a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.
Entrez Gene ID
Human26986
Chicken430997
Rabbit100342434
Frog448595
UniProt ID
HumanP11940
ChickenA0A1D5NYB2
FrogP20965
Alternative Names
Poly(A) Binding Protein Cytoplasmic 1; PABPC2; PABP1; PAB1; Poly(A)-Binding Protein, Cytoplasmic 2; Poly(A) Binding Protein, Cytoplasmic 2;
Function
Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability (PubMed:11051545, PubMed:17212783, PubMed:25480299).

Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2 (PubMed:11051545, PubMed:20573744).

Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover (PubMed:11051545).

Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed:11051545).

Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (PubMed:18447585).

By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (PubMed:25480299).
(Microbial infection) Positively regulates the replication of dengue virus (DENV).
Biological Process
Gene silencing by RNA Source: UniProtKB
mRNA polyadenylation Source: UniProtKB
mRNA splicing, via spliceosome Source: UniProtKB
mRNA stabilization Source: UniProtKB
Negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
Positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: UniProtKB
Positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: UniProtKB
Positive regulation of viral genome replication Source: UniProtKB
Regulation of mRNA stability Source: Reactome
Translational initiation Source: Reactome
Cellular Location
Cytoplasm; Nucleus; Stress granule; Lamellipodium. Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:17289661). Shuttles between the cytoplasm and the nucleus (PubMed:9582337). During stress and in the absence of DDX3X, localizes to the nucleus (PubMed:21883093). At the leading edge of migrating fibroblasts, colocalizes with DDX3X (PubMed:28733330). Relocalizes to cytoplasmic stress granules upon cellular stress where it colocalizes with ENDOV (PubMed:27573237). In case of HRSV infection, localizes in cytoplasmic inclusion bodies substructures called inclusion bodies associated granules (IBAGs) (PubMed:31649314).
PTM
Phosphorylated by MAPKAPK2.
Methylated by CARM1. Arg-493 is dimethylated, probably to asymmetric dimethylarginine.

Lim, K., Palo, M. Z., Puglisi, J. D., & Puglisi, E. V. (2024). Role of human PABPC1 in canonical and viral translation initiation. Biophysical Journal, 123(3), 362a.

Meng, X., Xiao, W., Sun, J., Li, W., Yuan, H., Yu, T., ... & Dong, W. (2023). CircPTK2/PABPC1/SETDB1 axis promotes EMT-mediated tumor metastasis and gemcitabine resistance in bladder cancer. Cancer Letters, 554, 216023.

Zhang, Y., Chen, C., Liu, Z., Guo, H., Lu, W., Hu, W., & Lin, Z. (2022). PABPC1-induced stabilization of IFI27 mRNA promotes angiogenesis and malignant progression in esophageal squamous cell carcinoma through exosomal miRNA-21-5p. Journal of Experimental & Clinical Cancer Research, 41(1), 111.

Qi, Y., Wang, M., & Jiang, Q. (2022). PABPC1——mRNA stability, protein translation and tumorigenesis. Frontiers in Oncology, 12, 1025291.

Wegler, M., Jia, X., Alders, M., Bouman, A., Chen, J., Duan, X., ... & Abou Jamra, R. (2022). De novo variants in the PABP domain of PABPC1 lead to developmental delay. Genetics in Medicine, 24(8), 1761-1773.

Feng, C., Han, Y. H., Qi, N., Li, J., Sheng, Q. H., Liu, Y., & Yang, L. L. (2021). Functional implications of PABPC1 in the development of ovarian cancer. Open Medicine, 16(1), 805-815.

An, T., Deng, L., Wang, Y., Yang, Z., Chai, C., Ouyang, J., ... & Zhang, C. (2021). The prognostic impacts of PABPC1 expression on gastric cancer patients. Future Oncology, 17(33), 4471-4479.

Smith, E. M., Benbahouche, N. E. H., Morris, K., Wilczynska, A., Gillen, S., Schmidt, T., ... & Bushell, M. (2021). The mTOR regulated RNA-binding protein LARP1 requires PABPC1 for guided mRNA interaction. Nucleic acids research, 49(1), 458-478.

YuFeng, Z., & Ming, Q. (2020). Expression and prognostic roles of PABPC1 in hepatocellular carcinoma. International Journal of Surgery, 84, 3-12.

Wang, Q., Wang, Z., Bao, Z., Zhang, C., Wang, Z., & Jiang, T. (2020). PABPC1 relevant bioinformatic profiling and prognostic value in gliomas. Future Oncology, 16(01), 4279-4288.

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For research use only. Not intended for any clinical use.

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