Mouse Anti-PARD3 Recombinant Antibody (4G5) (CBMAB-P0768-YC)

Name: Mouse Anti-PARD3 Recombinant Antibody (4G5)
SKU: CBMAB-P0768-YC
Rating: 5 (1 reviews)

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Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4G5

Application

WB, IP, ELISA

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

par-3 partitioning defective 3 homolog (C. elegans)

Introduction

PARD3 is a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene.

Entrez Gene ID

56288

UniProt ID

Q8TEW0

Alternative Names

Par-3 Family Cell Polarity Regulator; Atypical PKC Isotype-Specific Interacting Protein; Protein Phosphatase 1, Regulatory Subunit 118; Par-3 Family Cell Polarity Regulator Alpha; CTCL Tumor Antigen Se2-5; PAR3-Alpha; PARD-3; PAR3; ASIP; Par-3 (Partitioning Defective 3, C.Elegans) Homolog; Par-3 Partitioning Defective 3 Homolog (C. Elegans); Atypical PKC Isotype-Specific-Interacting Protein; Par-3 Partitioning Defective 3 Homolog;

Function

Adapter protein involved in asymmetrical cell division and cell polarization processes (PubMed:27925688, PubMed:10954424).
Seems to play a central role in the formation of epithelial tight junctions (PubMed:27925688).
Targets the phosphatase PTEN to cell junctions (By similarity).
Involved in Schwann cell peripheral myelination (By similarity).
Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (By similarity).
The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (PubMed:10934474).
Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (PubMed:19812038, PubMed:27925688).

Biological Process

Asymmetric cell divisionManual Assertion Based On ExperimentTAS:ProtInc
AxonogenesisManual Assertion Based On ExperimentTAS:UniProtKB
Bicellular tight junction assemblyISS:UniProtKB
Cell adhesionManual Assertion Based On ExperimentIBA:GO_Central
Cell cycleIEA:UniProtKB-KW
Establishment of cell polarityManual Assertion Based On ExperimentIBA:GO_Central
Establishment of centrosome localizationManual Assertion Based On ExperimentIBA:GO_Central
Establishment of epithelial cell polarityISS:UniProtKB
Establishment or maintenance of cell polarityManual Assertion Based On ExperimentTAS:UniProtKB
Establishment or maintenance of epithelial cell apical/basal polarityManual Assertion Based On ExperimentIDA:ComplexPortal
Microtubule cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Myelination in peripheral nervous systemISS:UniProtKB
Negative regulation of peptidyl-threonine phosphorylationISS:UniProtKB
Positive regulation of myelinationISS:UniProtKB
Protein kinase C-activating G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentTAS:UniProtKB
Protein localizationManual Assertion Based On ExperimentIBA:GO_Central
Protein targeting to membraneISS:UniProtKB
Protein-containing complex assemblyManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cytoplasm By Similarity
Endomembrane system 1 Publication
Cell junction 2 Publications
Cell junction, tight junction 2 Publications
Cell junction, adherens junction By Similarity
Cell membrane 2 Publications
Cytoplasm, cell cortex By Similarity
Cytoplasm, cytoskeleton 1 Publication
Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II. Colocalized with SIRT2 in internode region of myelin sheath (By similarity).
Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.

Involvement in disease

Neural tube defects (NTD):
Congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy. Failure of neural tube closure can occur at any level of the embryonic axis. Common NTD forms include anencephaly, myelomeningocele and spina bifida, which result from the failure of fusion in the cranial and spinal region of the neural tube. NTDs have a multifactorial etiology encompassing both genetic and environmental components.

PTM

Acetylated. Deacetylated by SIRT2, thereby inhibiting Schwann cell peripheral myelination.
Phosphorylation at Ser-827 by PRKCZ and PRKCI occurs at the most apical tip of epithelial cell-cell contacts during the initial phase of tight junction formation and may promote dissociation of the complex with PARD6. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2 (By similarity).
Phosphorylation by AURKA at Ser-962 is required for the normal establishment of neuronal polarity (PubMed:19812038).

Chen, S., Paul, M. R., Sterner, C. J., Belka, G. K., Wang, D., Xu, P., ... & Chodosh, L. A. (2023). PAQR8 promotes breast cancer recurrence and confers resistance to multiple therapies. Breast Cancer Research, 25(1), 1.

Hornung, R. S., Kinchington, P. R., Umorin, M., & Kramer, P. R. (2023). PAQR8 and PAQR9 expression is altered in the ventral tegmental area of aged rats infected with varicella zoster virus. Molecular Pain, 19, 17448069231202598.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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